1|Page
KU BIOL 600 EXAM 1 Review Questions, Biochem
final
____________ is a fibrous protein and is the primary component of
wool and hair - . . ANSWER ✓✓ alpha keratin
______________ may be effective anti-cancer drugs - . . ANSWER
✓✓ Protein kinase inhibitors
_________________________ _refers to the spatial arrangement of
polypeptide subunits and the nature of their interactions - . .
ANSWER ✓✓ quaternary structure
. In the Edman method for protein sequencing, phenyl
isothiocyanate is used to selectively remove the
________________ residue. - . . ANSWER ✓✓ amino terminal/N-
terminal
....are cells which are composed of multiple specialized
membrane-bound
compartments. - . . ANSWER ✓✓ Eukaryotic cells
,2|Page
...weak interaction between water molecules. - . . ANSWER ✓✓
hydrogen bonding
"Starting from glucose, arrange the following metabolites in the
order of their biosynthesis during glycolysis" - . . ANSWER ✓✓ 1.
glucose-6-phosphate
2. fructose-6-phosphate
3. "fructose-1,6-biphosphate"
4. glyceraldehyde-3-phosphate
5. phosphoenolpyruvate
6. pyruvate
2,3-Bisphosphoglycerate - . . ANSWER ✓✓ binds in the central
cavity in the T-form of hemoglobin.
preferentially binds to deoxyhemoglobin and stabilizes it.
is present in the red blood cells.
All of the above.
3 unsaturated fatty acids. These ____________________ fatty acids
are precursors in the
biosynthesis of hormones known as ____________________________.
- . . ANSWER ✓✓ omega-3
Prostaglandins
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A mixture of proteins of nearly the same MWs are to be
separated to purify a protein that is rich in Arg
and Lys residues. What chromatographic method will you use? - .
. ANSWER ✓✓ ion exchange chromatography or cation exchange
chromatography
A nickel column used in purifying proteins will bind proteins that
contain a ____________ tag - . . ANSWER ✓✓ histidine (His)
A protein is considered to be _________________when it is
converted into a random coil structure without its
normal activity. - . . ANSWER ✓✓ denatured
Adenylate cyclase converts this substrate _________________ into
cyclic AMP - . . ANSWER ✓✓ ATP
All of the following are repeating units of glycosaminoglycans
except:
chondroitin
6-sulfate
keratin sulfate
hyaluronate
heparin
selectin - . . ANSWER ✓✓ keratin sulfate
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Alzheimer's disease is one of many diseases associated with
protein - . . ANSWER ✓✓ misfolding
Amino acid with imidazole side chains - . . ANSWER ✓✓
Histidine
Amino acid with pKa near neutral pH - . . ANSWER ✓✓ Histidine
Among the weak interactions encountered in biochemistry, this
is the weakest interaction - . . ANSWER ✓✓ hydrophobic
An amino acid which must be supplied by the diet. - . . ANSWER
✓✓ essential amino acid
An enzyme's affinity for its substrate is measured by what
parameter? - . . ANSWER ✓✓ KM (the Michaelis-Menten
constant)
Another name for molecules with both (+) and (-) charges. - . .
ANSWER ✓✓ Zwitter ions
Arrange the following in order of strength, from top (#1) to
bottom: hydrogen bonding, electrostatic
KU BIOL 600 EXAM 1 Review Questions, Biochem
final
____________ is a fibrous protein and is the primary component of
wool and hair - . . ANSWER ✓✓ alpha keratin
______________ may be effective anti-cancer drugs - . . ANSWER
✓✓ Protein kinase inhibitors
_________________________ _refers to the spatial arrangement of
polypeptide subunits and the nature of their interactions - . .
ANSWER ✓✓ quaternary structure
. In the Edman method for protein sequencing, phenyl
isothiocyanate is used to selectively remove the
________________ residue. - . . ANSWER ✓✓ amino terminal/N-
terminal
....are cells which are composed of multiple specialized
membrane-bound
compartments. - . . ANSWER ✓✓ Eukaryotic cells
,2|Page
...weak interaction between water molecules. - . . ANSWER ✓✓
hydrogen bonding
"Starting from glucose, arrange the following metabolites in the
order of their biosynthesis during glycolysis" - . . ANSWER ✓✓ 1.
glucose-6-phosphate
2. fructose-6-phosphate
3. "fructose-1,6-biphosphate"
4. glyceraldehyde-3-phosphate
5. phosphoenolpyruvate
6. pyruvate
2,3-Bisphosphoglycerate - . . ANSWER ✓✓ binds in the central
cavity in the T-form of hemoglobin.
preferentially binds to deoxyhemoglobin and stabilizes it.
is present in the red blood cells.
All of the above.
3 unsaturated fatty acids. These ____________________ fatty acids
are precursors in the
biosynthesis of hormones known as ____________________________.
- . . ANSWER ✓✓ omega-3
Prostaglandins
,3|Page
A mixture of proteins of nearly the same MWs are to be
separated to purify a protein that is rich in Arg
and Lys residues. What chromatographic method will you use? - .
. ANSWER ✓✓ ion exchange chromatography or cation exchange
chromatography
A nickel column used in purifying proteins will bind proteins that
contain a ____________ tag - . . ANSWER ✓✓ histidine (His)
A protein is considered to be _________________when it is
converted into a random coil structure without its
normal activity. - . . ANSWER ✓✓ denatured
Adenylate cyclase converts this substrate _________________ into
cyclic AMP - . . ANSWER ✓✓ ATP
All of the following are repeating units of glycosaminoglycans
except:
chondroitin
6-sulfate
keratin sulfate
hyaluronate
heparin
selectin - . . ANSWER ✓✓ keratin sulfate
, 4|Page
Alzheimer's disease is one of many diseases associated with
protein - . . ANSWER ✓✓ misfolding
Amino acid with imidazole side chains - . . ANSWER ✓✓
Histidine
Amino acid with pKa near neutral pH - . . ANSWER ✓✓ Histidine
Among the weak interactions encountered in biochemistry, this
is the weakest interaction - . . ANSWER ✓✓ hydrophobic
An amino acid which must be supplied by the diet. - . . ANSWER
✓✓ essential amino acid
An enzyme's affinity for its substrate is measured by what
parameter? - . . ANSWER ✓✓ KM (the Michaelis-Menten
constant)
Another name for molecules with both (+) and (-) charges. - . .
ANSWER ✓✓ Zwitter ions
Arrange the following in order of strength, from top (#1) to
bottom: hydrogen bonding, electrostatic
