ASCP CG CERTIFICATION PRACTICE EXAM
WITH CORRECT ACTUAL QUESTIONS AND
CORRECTLY WELL DEFINED ANSWERS
LATEST ALREADY GRADED A+ 2025 – 2026
What should be used on a cultured CVS sample to obtain more
slides for analysis? - ANSWERS-Trypsin-EDTA -- to remove adherent
cells from the flask or coverslip to split into more vessels
EDTA improves tryp's effectiveness (chelating agent)
Cyto studies were ordered on a prenatal patient at 12 weeks
gestation after an abnormality was detected via ultrasound. 2 of 15
colonies from seperate primary cultures showed an abnormal cell
line of 47,XX,+21. The next step would be to ___ ? - ANSWERS-
Report the result as this is a true mosaic case and suggest:
repeating amniocentesis,
repeating a detailed ultrasound, or
request percutaneous umbilical blood sample (PUBS)
,After staining, the chromosomes appear to have indistinct bands
with little contrast. The problem is corrected by: - ANSWERS-
Increasing trypsin exposure (chroms were muddy and dark)
When scoring FISH for DiGeorge syndrome, a tech notices the
TUPLE1 signal is present, but one of the control ARSA probe signals
is missing in every cell. This result suggests __ ? - ANSWERS-The
patient has a deletion of the ARSA region
CLIA 1988- (Clinical Laboratory Improvement Amendments)
stipulates that equipment must be recalibrated __ ? - ANSWERS-
Every 6 months (Standard 493.1217)
What type of stained chromosome would produce a better
computer image? - ANSWERS-Lightly stained chromosomes (best
contrast and can be digitally edited easier)
What is the nomenclature on a patient with down syndrome
referred for a hematologic disorder where only trisomy 21 was
found? - ANSWERS-47,XY,+21c[20]
24 hours after culture initiation, a single dish from an amniotic fluid
turns cloudy. Which cultures should be disposed? - ANSWERS-The
single cloudy dish only
,Phytohemagglutinin (PHA) stimulates which type of cells into
division? - ANSWERS-T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When
logging a culture failure, what is the MOST important to document?
- ANSWERS-Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic
villus) sample? - ANSWERS-Dissection
Which cell type is most problematic if found in a CVS (chorionic
villus) sample? - ANSWERS-Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of
cytoplasm is still seen around the mets, what is the most likely
cause? - ANSWERS-innappropriate slide drying time
What will help prevent microbial contamination and cross
contamination of cultures? - ANSWERS-Asceptic technique
When there are not enough mets to complete the study of a
patient/case, what needs to be done next? - ANSWERS-prepare
additional slides from any remaining sample
, When looking at a dropped slide under a phase scope and mets are
very spread out and sparse, what could help fix the issue? -
ANSWERS-Increading the drying time by using warm slides
What is true of an inverted microscope? - ANSWERS-the objectives
are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: -
ANSWERS-DiGenorge syndrome -- microdeletion of 22q11, also
called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip
indent), tubular nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic
analysis, what is the MOST important data to accompany the
specimen? - ANSWERS-Reason for referral
Which type of specimen should be requested for a cytogenetic
analysis for turner syndrome? - ANSWERS-Heparinized peripheral
blood stored at room temperature
(NOT FROZEN)
WITH CORRECT ACTUAL QUESTIONS AND
CORRECTLY WELL DEFINED ANSWERS
LATEST ALREADY GRADED A+ 2025 – 2026
What should be used on a cultured CVS sample to obtain more
slides for analysis? - ANSWERS-Trypsin-EDTA -- to remove adherent
cells from the flask or coverslip to split into more vessels
EDTA improves tryp's effectiveness (chelating agent)
Cyto studies were ordered on a prenatal patient at 12 weeks
gestation after an abnormality was detected via ultrasound. 2 of 15
colonies from seperate primary cultures showed an abnormal cell
line of 47,XX,+21. The next step would be to ___ ? - ANSWERS-
Report the result as this is a true mosaic case and suggest:
repeating amniocentesis,
repeating a detailed ultrasound, or
request percutaneous umbilical blood sample (PUBS)
,After staining, the chromosomes appear to have indistinct bands
with little contrast. The problem is corrected by: - ANSWERS-
Increasing trypsin exposure (chroms were muddy and dark)
When scoring FISH for DiGeorge syndrome, a tech notices the
TUPLE1 signal is present, but one of the control ARSA probe signals
is missing in every cell. This result suggests __ ? - ANSWERS-The
patient has a deletion of the ARSA region
CLIA 1988- (Clinical Laboratory Improvement Amendments)
stipulates that equipment must be recalibrated __ ? - ANSWERS-
Every 6 months (Standard 493.1217)
What type of stained chromosome would produce a better
computer image? - ANSWERS-Lightly stained chromosomes (best
contrast and can be digitally edited easier)
What is the nomenclature on a patient with down syndrome
referred for a hematologic disorder where only trisomy 21 was
found? - ANSWERS-47,XY,+21c[20]
24 hours after culture initiation, a single dish from an amniotic fluid
turns cloudy. Which cultures should be disposed? - ANSWERS-The
single cloudy dish only
,Phytohemagglutinin (PHA) stimulates which type of cells into
division? - ANSWERS-T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When
logging a culture failure, what is the MOST important to document?
- ANSWERS-Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic
villus) sample? - ANSWERS-Dissection
Which cell type is most problematic if found in a CVS (chorionic
villus) sample? - ANSWERS-Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of
cytoplasm is still seen around the mets, what is the most likely
cause? - ANSWERS-innappropriate slide drying time
What will help prevent microbial contamination and cross
contamination of cultures? - ANSWERS-Asceptic technique
When there are not enough mets to complete the study of a
patient/case, what needs to be done next? - ANSWERS-prepare
additional slides from any remaining sample
, When looking at a dropped slide under a phase scope and mets are
very spread out and sparse, what could help fix the issue? -
ANSWERS-Increading the drying time by using warm slides
What is true of an inverted microscope? - ANSWERS-the objectives
are beneath the stage
High-resolution banding is indicated for a possible diagnosis of: -
ANSWERS-DiGenorge syndrome -- microdeletion of 22q11, also
called velocardiofacial syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip
indent), tubular nose, wide spread eyes, etc
When verifying if a specimen is appropriate for cytogenetic
analysis, what is the MOST important data to accompany the
specimen? - ANSWERS-Reason for referral
Which type of specimen should be requested for a cytogenetic
analysis for turner syndrome? - ANSWERS-Heparinized peripheral
blood stored at room temperature
(NOT FROZEN)
