ACTUAL Exam Questions and CORRECT
Answers
T/F: Growth media is best suited for distinguishing between two similar species of bacteria -
CORRECT ANSWER - False
growth media is designed to simply support (and not restrict) microbial growth
A researcher is asked to determine which of two vials contains E coli and which contains
salmonella. Knowing both are Gram-negative while only one of them is capable of fermenting
lactose, which type of media would be best suited? - CORRECT ANSWER - Differential
media
What are the requirements of a fastidious microbe? - CORRECT ANSWER - A fastidious
microbe is an organism with complex growth requirements and will not grow without then.
Enriched medias thus contain these specific and essential nutrients required for the growth of a
particular subset of microorganisms.
T/F: LB agar is classified as a non-selective, non-differential media - CORRECT
ANSWER - True
The polysaccharide-based hardening agent added to LB media to produce LB agar is derived
from what? - CORRECT ANSWER - Seaweed extract
Blood agar is which type of medium? Select all that apply:
- Selective and Differential
- Enriched
- Differential
- Selective - CORRECT ANSWER - - Enriched
, - Differential
Match the following hemolytic class with its description of activity:
1. Alpha hemolysis
2. Beta hemolysis
3. Gamma hemolysis
A. No change
B. Greenish-brown color
C. Distinct zone of clearing - CORRECT ANSWER - 1. Alpha hemolysis- Greenish-
brown color (B)
2. Beta hemolysis- Distinct zone of clearing (C)
3. Gamma hemolysis- No change (A)
Match the following hemolytic class with its description of activity:
1. Alpha hemolysis
2. Beta hemolysis
3. Gamma hemolysis
A. No hemolytic activity
B. Incomplete hemolytic activity
C. Complete hemolytic activity - CORRECT ANSWER - 1. Alpha hemolysis- Incomplete
hemolytic activity (B)
2. Beta hemolysis- Complete hemolytic activity (C)
3. Gamma hemolysis- No hemolytic activity (A)
Columbia CNA agar is most closely related to which media: - CORRECT ANSWER -
Blood agar