MB ASCP CONNECT EXAM 2025 UPDATE QUESTIONS AND CORRECT
VERIFIED ANSWERS ALREADY GRADED A+ (BRAND NEW VISION)
While at the doctor's office with your father, you overheard his physician tell another physician
that test results came in, confirming the presence of the Factor V Leiden mutation, a mutation
associated with deep venous thrombosis. Which of the following is the mutation your father
has:
a. A1691G
b. G1619A
c. 1691G>A
d. C282Y - answers1691G>A
Which of the following is not involved in the splicing reaction?
a. 5' splice site
b. Hairpin loops
c. Branch A point
d. 3' splice site - answersHairpin loops
The amelogenin locus, found on the sex chromosomes, is used in gender identification.
Amplification at this locus reveals 2 peaks of sizes 212 and 218 base pairs. If this amplification
were part of a gender identification procedure, what would be the gender of the individual?
a. Male
b. Female
c. Hermaphrodite
,d. Can't be determined from the given information - answersMale
TIP: Females 106/112 (typically shorter than males)
In CML, what fusion protein is created?
a. EWS-FLI1
b. EWS-PAR2
c. BCR-Abl1
d. c-myc-IGH - answersBCR-Abl1
Which of the following is not a step in the Southern blot procedure?
a. DNA digest with restriction enzymes
b. Gel resolution of fragments
c. Fragments denaturation
d. Probe digest - answersProbe digest
This polymerase acts on DNA and produces Messenger RNA:
a. RNA Pol I
b. DNA Pol I
c. RNA Pol II
d. DNA Pol II - answersRNA Pol II
TIP: mRNA comes later so 2.
,Which of the following will more likely lower stringency conditions in the washing step of a
hybridization experiment?
a. Increase the concentration of salt in the wash solution buffer
b. Increase the temperature from, say 68°C to 75°C
c. Use a probe with a higher density of GC base pairs as compared to one with a lower GC base
pair density
d. Remove formamide from the wash solution buffer - answersIncrease the concentration of
salt in the wash solution buffer
Bone marrow transplantation is a great method used to treat several malignant and benign
cancers, particularly leukemias. Before transplantation (or engraftment analysis), several
polymorphic loci are screened in both the recipient and donor cells. The main goal of this
screening is to:
a. find at least one or more informative loci between both the donor and recipient
b. find exactly ten informative loci between both the donor and recipient
c. find all the non-informative loci between both the donor and recipient
d. find the blood type/group of both the donor and recipient - answersfind at least one or more
informative loci between both the donor and recipient
The ideal spacing between the -10 and -35 regions of a prokaryotic promoter is:
a. 14
b. 15
c. 16
d. 17 - answers17
, How do you detect RFLP?
a. PCR
b. Southern Blot
c. Northern Blot
d. Western Blot - answersSouthern Blot
How do you detect VNTRs?
a. PCR
b. Southern Blot
c. Northern Blot
d. Western Blot - answersPCR & Southern Blot
How do you detect STRs?
a. PCR
b. Southern Blot
c. Northern Blot
d. Western Blot - answersPCR
How do you detect SNPs?
a. Sequencing
b. Southern Blot
VERIFIED ANSWERS ALREADY GRADED A+ (BRAND NEW VISION)
While at the doctor's office with your father, you overheard his physician tell another physician
that test results came in, confirming the presence of the Factor V Leiden mutation, a mutation
associated with deep venous thrombosis. Which of the following is the mutation your father
has:
a. A1691G
b. G1619A
c. 1691G>A
d. C282Y - answers1691G>A
Which of the following is not involved in the splicing reaction?
a. 5' splice site
b. Hairpin loops
c. Branch A point
d. 3' splice site - answersHairpin loops
The amelogenin locus, found on the sex chromosomes, is used in gender identification.
Amplification at this locus reveals 2 peaks of sizes 212 and 218 base pairs. If this amplification
were part of a gender identification procedure, what would be the gender of the individual?
a. Male
b. Female
c. Hermaphrodite
,d. Can't be determined from the given information - answersMale
TIP: Females 106/112 (typically shorter than males)
In CML, what fusion protein is created?
a. EWS-FLI1
b. EWS-PAR2
c. BCR-Abl1
d. c-myc-IGH - answersBCR-Abl1
Which of the following is not a step in the Southern blot procedure?
a. DNA digest with restriction enzymes
b. Gel resolution of fragments
c. Fragments denaturation
d. Probe digest - answersProbe digest
This polymerase acts on DNA and produces Messenger RNA:
a. RNA Pol I
b. DNA Pol I
c. RNA Pol II
d. DNA Pol II - answersRNA Pol II
TIP: mRNA comes later so 2.
,Which of the following will more likely lower stringency conditions in the washing step of a
hybridization experiment?
a. Increase the concentration of salt in the wash solution buffer
b. Increase the temperature from, say 68°C to 75°C
c. Use a probe with a higher density of GC base pairs as compared to one with a lower GC base
pair density
d. Remove formamide from the wash solution buffer - answersIncrease the concentration of
salt in the wash solution buffer
Bone marrow transplantation is a great method used to treat several malignant and benign
cancers, particularly leukemias. Before transplantation (or engraftment analysis), several
polymorphic loci are screened in both the recipient and donor cells. The main goal of this
screening is to:
a. find at least one or more informative loci between both the donor and recipient
b. find exactly ten informative loci between both the donor and recipient
c. find all the non-informative loci between both the donor and recipient
d. find the blood type/group of both the donor and recipient - answersfind at least one or more
informative loci between both the donor and recipient
The ideal spacing between the -10 and -35 regions of a prokaryotic promoter is:
a. 14
b. 15
c. 16
d. 17 - answers17
, How do you detect RFLP?
a. PCR
b. Southern Blot
c. Northern Blot
d. Western Blot - answersSouthern Blot
How do you detect VNTRs?
a. PCR
b. Southern Blot
c. Northern Blot
d. Western Blot - answersPCR & Southern Blot
How do you detect STRs?
a. PCR
b. Southern Blot
c. Northern Blot
d. Western Blot - answersPCR
How do you detect SNPs?
a. Sequencing
b. Southern Blot
