MLT 106 ABO / Rh / Testing Reagents -
WT3 - 68K Army Navy Lab Tech UPDATED
ACTUAL Exam Questions and CORRECT
Answers
Isohemagglutinins - CORRECT ANSWER - Antibodies against Antigens absent from own
RBCs
"naturally occurring" - ssg paupard
form against similarly structured antibodies from *ENVIRONMENTAL* gut bacteria
Age at which isohemagglutinins first appear and peak - CORRECT ANSWER - 3-6
months of age
5-10 years
Landsteiner's Rules - CORRECT ANSWER - Ag present on cells -> Ab absent in serum
Ab present serum -> Ag absent on cells
ABO Expression - CORRECT ANSWER - RBCs, endothelium, Kidney, Heart, Bowel,
Pancreas, Lungs
Results of Incompatible ABO Transfusion - CORRECT ANSWER - acute intravascular
hemolysis and renal failure,
fatal
,MABs benefits - CORRECT ANSWER - Reproducibility major advantage
same specificity and affinity
unwanted are excluded (purity)
Reagent Colors - CORRECT ANSWER - Blue - Anti-A
Yellow - Anti-B
Forward and Reverse Type Relationship - CORRECT ANSWER - inverse reciprocal
relationship
Forward grouping (front type) - CORRECT ANSWER - testing for pt unknown Ags using
known Antisera
Anti-A and Anti-B
No agglut - negative, ag absent
Agglut - pos, specific antigen present
Reverse Grouping - CORRECT ANSWER - test for pt unknown Abs via known reagent
Ags (cells)
2-4% suspensions of A1 cells or B cells
No agglut - negative, Ab absent
Agglut - positive, Ab present
, Age Effect on Ig and Reverse Typing - CORRECT ANSWER - Low IG production with
age can cause low reverse typing
LECTINS for BBK - CORRECT ANSWER - *Dolichos biflorus* - A1 / A1B
agglutination
*Bandeiraea simplicfiolia* - agglutinates B Cells
*Ulex europaeus* - O cells (H specificity) and ABO groups depending on H (Bombay grouping)
Major Abs of ABO System - CORRECT ANSWER - ANTI-
A
B
A,B
H
Major Ag of ABO System - CORRECT ANSWER -A
B
H (O) - as O is absent
ABO Antibody Class - CORRECT ANSWER - Primarily IgM
Anti-A,B - IgG
Chart pg 81 - CORRECT ANSWER - know population which has highest and lowest of
each ABO phenotype
WT3 - 68K Army Navy Lab Tech UPDATED
ACTUAL Exam Questions and CORRECT
Answers
Isohemagglutinins - CORRECT ANSWER - Antibodies against Antigens absent from own
RBCs
"naturally occurring" - ssg paupard
form against similarly structured antibodies from *ENVIRONMENTAL* gut bacteria
Age at which isohemagglutinins first appear and peak - CORRECT ANSWER - 3-6
months of age
5-10 years
Landsteiner's Rules - CORRECT ANSWER - Ag present on cells -> Ab absent in serum
Ab present serum -> Ag absent on cells
ABO Expression - CORRECT ANSWER - RBCs, endothelium, Kidney, Heart, Bowel,
Pancreas, Lungs
Results of Incompatible ABO Transfusion - CORRECT ANSWER - acute intravascular
hemolysis and renal failure,
fatal
,MABs benefits - CORRECT ANSWER - Reproducibility major advantage
same specificity and affinity
unwanted are excluded (purity)
Reagent Colors - CORRECT ANSWER - Blue - Anti-A
Yellow - Anti-B
Forward and Reverse Type Relationship - CORRECT ANSWER - inverse reciprocal
relationship
Forward grouping (front type) - CORRECT ANSWER - testing for pt unknown Ags using
known Antisera
Anti-A and Anti-B
No agglut - negative, ag absent
Agglut - pos, specific antigen present
Reverse Grouping - CORRECT ANSWER - test for pt unknown Abs via known reagent
Ags (cells)
2-4% suspensions of A1 cells or B cells
No agglut - negative, Ab absent
Agglut - positive, Ab present
, Age Effect on Ig and Reverse Typing - CORRECT ANSWER - Low IG production with
age can cause low reverse typing
LECTINS for BBK - CORRECT ANSWER - *Dolichos biflorus* - A1 / A1B
agglutination
*Bandeiraea simplicfiolia* - agglutinates B Cells
*Ulex europaeus* - O cells (H specificity) and ABO groups depending on H (Bombay grouping)
Major Abs of ABO System - CORRECT ANSWER - ANTI-
A
B
A,B
H
Major Ag of ABO System - CORRECT ANSWER -A
B
H (O) - as O is absent
ABO Antibody Class - CORRECT ANSWER - Primarily IgM
Anti-A,B - IgG
Chart pg 81 - CORRECT ANSWER - know population which has highest and lowest of
each ABO phenotype
