08/08/2025, 12:16 MICR 4423 Exam 5 QUESTIONS AND VERIFIED ANSWERS 2025(GRADED A+) DETAILED ANSWERS!! Flashcards | Quizlet
MICR 4423 Exam 5 QUESTIONS AND VERIFIED
ANSWERS 2025(GRADED A+) DETAILED
ANSWERS!!
Save
Terms in this set (67)
What is antibiotic Survival of a population of antibiotic resistant cells
selection? within a larger population of antibiotic sensitive cells.
Anywhere where there are antibiotics and bacteria
Where can antibiotic
present. The best-surviving bacteria will be selected
selection occur?
for.
Name a common use for To identify and isolate cells that have piece of DNA of
antibiotic selection in interest such as a particular plasmid or gene in the
microbiological research. chromosome.
Why does virtually every It allows the cells that contain that plasmid to be
plasmid used for scientific identified through antibiotic selection.
research contain an
antibiotic resistance
marker?
Imagine that you have a Plate the cells on an ampicillin plate so that only the
sample of bacteria that is a ampicillin-resistant cells will survive and produce
mixture of ampicillin- colonies.
resistant cells and
ampicillin-sensitive cells.
How could you isolate the
ampicillin-resistant cells?
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, 08/08/2025, 12:16 MICR 4423 Exam 5 QUESTIONS AND VERIFIED ANSWERS 2025(GRADED A+) DETAILED ANSWERS!! Flashcards | Quizlet
What antibiotic or class of Bla- ampicillin/any cillin
antibiotics do cells Aac- aminoglycosides (usually kanamycin)
containing the bla marker Cat- chloramphenicol
become resistant to? What
about aac? What about
cat?
What are the Tet-ON and To inducibly or conditionally control gene expression
Tet-OFF systems typically in eukaryotic systems.
used for?
What is a typical inducer Plain tetracycline or (usually doxycycline)
of the Tet-ON or Tet-OFF
system?
In Tet-ON when you add tet or dox it turns gene
Distinguish between the
expression on. In Tet-OFF when you add tet or dox it
Tet-ON and Tet-OFF
turns tet expression off. Addition of tetracycline
systems in terms of what
always causes tetR to come off its operator. In Tet-
inducer addition does to
OFF there is a cruddy promoter that requires a
gene expression and how
transactivation domain which is bound to TetR. When
it occurs at the molecular
TetR leaves it takes the transactivation domain with it
level.
and stops gene expression.
What is a typical way of Through its drinking water.
administering Tet to an
animal?
Contrast how antibiotics In US- prescribed by a doctor and dispensed by a
are regulated in the pharmacist.
United States and Most other countries- over-the-counter in drug stores
northern Europe with how or even gas stations.
they are regulated in most
other countries.
https://quizlet.com/1064577726/micr-4423-exam-5-questions-and-verified-answers-2025graded-a-detailed-answers-flash-cards/?new 2/13
MICR 4423 Exam 5 QUESTIONS AND VERIFIED
ANSWERS 2025(GRADED A+) DETAILED
ANSWERS!!
Save
Terms in this set (67)
What is antibiotic Survival of a population of antibiotic resistant cells
selection? within a larger population of antibiotic sensitive cells.
Anywhere where there are antibiotics and bacteria
Where can antibiotic
present. The best-surviving bacteria will be selected
selection occur?
for.
Name a common use for To identify and isolate cells that have piece of DNA of
antibiotic selection in interest such as a particular plasmid or gene in the
microbiological research. chromosome.
Why does virtually every It allows the cells that contain that plasmid to be
plasmid used for scientific identified through antibiotic selection.
research contain an
antibiotic resistance
marker?
Imagine that you have a Plate the cells on an ampicillin plate so that only the
sample of bacteria that is a ampicillin-resistant cells will survive and produce
mixture of ampicillin- colonies.
resistant cells and
ampicillin-sensitive cells.
How could you isolate the
ampicillin-resistant cells?
https://quizlet.com/1064577726/micr-4423-exam-5-questions-and-verified-answers-2025graded-a-detailed-answers-flash-cards/?new 1/13
, 08/08/2025, 12:16 MICR 4423 Exam 5 QUESTIONS AND VERIFIED ANSWERS 2025(GRADED A+) DETAILED ANSWERS!! Flashcards | Quizlet
What antibiotic or class of Bla- ampicillin/any cillin
antibiotics do cells Aac- aminoglycosides (usually kanamycin)
containing the bla marker Cat- chloramphenicol
become resistant to? What
about aac? What about
cat?
What are the Tet-ON and To inducibly or conditionally control gene expression
Tet-OFF systems typically in eukaryotic systems.
used for?
What is a typical inducer Plain tetracycline or (usually doxycycline)
of the Tet-ON or Tet-OFF
system?
In Tet-ON when you add tet or dox it turns gene
Distinguish between the
expression on. In Tet-OFF when you add tet or dox it
Tet-ON and Tet-OFF
turns tet expression off. Addition of tetracycline
systems in terms of what
always causes tetR to come off its operator. In Tet-
inducer addition does to
OFF there is a cruddy promoter that requires a
gene expression and how
transactivation domain which is bound to TetR. When
it occurs at the molecular
TetR leaves it takes the transactivation domain with it
level.
and stops gene expression.
What is a typical way of Through its drinking water.
administering Tet to an
animal?
Contrast how antibiotics In US- prescribed by a doctor and dispensed by a
are regulated in the pharmacist.
United States and Most other countries- over-the-counter in drug stores
northern Europe with how or even gas stations.
they are regulated in most
other countries.
https://quizlet.com/1064577726/micr-4423-exam-5-questions-and-verified-answers-2025graded-a-detailed-answers-flash-cards/?new 2/13
