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What does sterilisation mean? Killing procedure of any kind of germs What does disinfection mean? Procedure where the number of the germs are reduced to a safety level. Which parameters can influence the effectivity of the sterilisation? The number of the germs, the resistance of the germs, the concentration of the disinfectants, the presence of the organic materials, the initial time, the presence of the biofilm. Parameters of the hot-air sterilisation cupboard protocol? 180°C; 1 hour, 160°C; 2 hours, 140°C; 3 hours. Parameters of the autoclaving? + 1 atm overpressure, 121°C, 20-30 minutes or 134°C, +2 atm overpressure 10 minutes. Chemical agents used for gas sterilisation? Etilene oxide, formaldehyde, beta-propiolacton The theoretical background of plasma sterilisation? Hydrogen-peroxide in high electric field will form plasma stage. The produced free radicals will kill the microbes. At the end of the procedure will be produced water, oxygen and other nontoxic products. Biological method used for checking the effectivity of the sterilisation. By Bacillus/Geobacillus stearothermophilus spores. If the procedure was performed in correct way, the spores cannot be cultivated. 8/8/25, 8:17 AM microbiology semmelweis Flashcards | Quizlet of pyrogenic material in drugs? LAL test; The blood of the horseshoe crab will coagulate in the presence of the LPS What are the disinfectants? Chemical agents used on inanimate/non-living surfaces. What are the antiseptic agents? Chemical agents used disinfection on animate (tissue, skin, mucous membrane) surfaces. 2. What does serological reaction mean? Reaction based on the antigen-antibody reaction performed in vitro. What does agglutination mean? Serological reaction where the antigen is cell mediated. What are the bacterial cell surface antigens? O: cell wall, H: flagella, K: capsule What does antibody titre mean? The highest dilution fold or the lowest antibody concentration where we can see in vitro antigenantibody reaction. What does precipitation mean? Serological reaction where the antigen is soluble (enzyme, toxin or virus particle). What does iatrogenic infection mean? Infection caused by medical staff during the investigation or treatment. What does nosocomial infection mean? Infection occurred in hospital after 48 hours of the hospitalisation What are the contents of the vaccines? (3 example) Live attenuated microbe; killed microbe, toxoid, antigens of the microbe. What does native examination of the microbe mean in microbiology? The microbe is examined without killing procedure. 8/8/25, 8:17 AM microbiology semmelweis Flashcards | Quizlet can we got by light microscopically examination? (3 example) The size of the microbe, the shape of the microbe, the motility, the staining can be examined. The solutions of the Gramstain? Sodium oxalate, cristal violet, Iodine solution, 96% of ethanol, fuchsin or safranin.

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8/8/25, 8:17 AM microbiology semmelweis Flashcards | Quizlet




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Terms in this set (50)


What does sterilisation Killing procedure of any kind of germs
mean?

What does disinfection Procedure where the number of the germs are
mean? reduced to a safety level.

The number of the germs, the resistance of the germs,
Which parameters can
the concentration of the disinfectants, the presence of
influence the effectivity of
the organic materials, the initial time, the presence of
the sterilisation?
the biofilm.

Parameters of the hot-air 180°C; 1 hour, 160°C; 2 hours, 140°C; 3 hours.
sterilisation cupboard
protocol?

Parameters of the + 1 atm overpressure, 121°C, 20-30 minutes or 134°C, +2
autoclaving? atm overpressure 10 minutes.

Chemical agents used for Etilene oxide, formaldehyde, beta-propiolacton
gas sterilisation?

Hydrogen-peroxide in high electric field will form
The theoretical plasma stage. The produced free radicals will kill the
background of plasma microbes. At the end of the procedure will be
sterilisation? produced water, oxygen and other nontoxic
products.

Biological method used By Bacillus/Geobacillus stearothermophilus spores. If
for checking the effectivity the procedure was performed in correct way, the
of the sterilisation. spores cannot be cultivated.
https://quizlet.com/1064555495/microbiology-semmelweis-flash-cards/?new 1/5

, 8/8/25, 8:17 AM microbiology semmelweis Flashcards | Quizlet


Detection of the presence LAL test; The blood of the horseshoe crab will
of pyrogenic material in coagulate in the presence of the LPS
drugs?

What are the Chemical agents used on inanimate/non-living
disinfectants? surfaces.

What are the antiseptic Chemical agents used disinfection on animate (tissue,
agents? skin, mucous membrane) surfaces.

2. What does serological Reaction based on the antigen-antibody reaction
reaction mean? performed in vitro.

What does agglutination Serological reaction where the antigen is cell
mean? mediated.

What are the bacterial cell O: cell wall, H: flagella, K: capsule
surface antigens?

The highest dilution fold or the lowest antibody
What does antibody titre
concentration where we can see in vitro antigen-
mean?
antibody reaction.

What does precipitation Serological reaction where the antigen is soluble
mean? (enzyme, toxin or virus particle).

What does iatrogenic Infection caused by medical staff during the
infection mean? investigation or treatment.

What does nosocomial Infection occurred in hospital after 48 hours of the
infection mean? hospitalisation

What are the contents of Live attenuated microbe; killed microbe, toxoid,
the vaccines? (3 example) antigens of the microbe.

What does native The microbe is examined without killing procedure.
examination of the
microbe mean in
microbiology?




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