Microbiology Lab UTA – Final Exam
2025 Questions and Answers
How is Media made? - --CORRECT ANSWER--5 g. T.S.A. and 125 mL Di water,
cover flask with foil, affix autoclave tape, autoclave, wait 10-20 min to cool, pour
into petri-dish to thickness of lid. or stop when covers bottom.
Agar - --CORRECT ANSWER--linear polysaccharide polymer derived from
agarose in red algae.
What does agar do? - --CORRECT ANSWER--increases solidity, exhibits
hysteresis- gels and melts at different temps. Melts above 85-90 and solidifies at 40-
45.
What is liquid media? - --CORRECT ANSWER--commonly called broths, doesn't
contain agar, easy to use, allows observation of growth patterns.
Advantages to using semi-solid media - --CORRECT ANSWER--To test oxygen
requirements, to test motility.
Fastidious - --CORRECT ANSWER--only grows in a fortified culture media under
specific conditions. The more _____________ the organism, the more specific
ingredients a medium must have. Uses defined media.
....COPYRIGHT ©️ 2025 ALL RIGHTS RESERVED...TRUSTED & VERIFIED 1
,Why use solid media? - --CORRECT ANSWER--The maintenance and growth of
stock cultures, to isolate single colonies, to ensure purity.
T.S.A. Plate - --CORRECT ANSWER--Tryptic Soy Agar most common medium
used in lab. Undefined media that contains casein digest, soybean digest, NaCl, and
agar.
What are the 4 biochemical families all living things are composed of? - --
CORRECT ANSWER--Proteins, Carbohydrates, lipids, nucleic acids
How much water does a bacteria cell consist of? - --CORRECT ANSWER--70%
Autoclave - --CORRECT ANSWER--sterilizes with steam at 121* C, pressure at
15 PSI for at least 15 min. Tested weekly to daily.
Reservoir - --CORRECT ANSWER--potential source of infection.
Normal commensal symbionts - --CORRECT ANSWER--S. pneumoniae and s.
aureus
Environmentally acquired microbes - --CORRECT ANSWER--p. aeruginosa and
b. cepacia
Magnifications of our microscopes - --CORRECT ANSWER--scanning: 4x-40,
low: 10x-100, high: 40x-400, oil immersion: 100x-1000
What is resolving power? - --CORRECT ANSWER--How close two objects can
be and still be distinguished as separate.
....COPYRIGHT ©️ 2025 ALL RIGHTS RESERVED...TRUSTED & VERIFIED 2
, What is the optimum resolution of the microscope using oil immersion? - --
CORRECT ANSWER--.2 micrometer.
How much does each microscope cost? - --CORRECT ANSWER--$1600
convex - --CORRECT ANSWER--Morphology of a domed colony
raised - --CORRECT ANSWER--flatter dome than convex
flat elevation - --CORRECT ANSWER--
crateriform - --CORRECT ANSWER--hole in convex colony
Umbonate - --CORRECT ANSWER--Morphology of a colony with a raised center
Rhizoid - --CORRECT ANSWER--Morphology of a branching colony
lobate - --CORRECT ANSWER--margin with lobes
undilated margins - --CORRECT ANSWER--irregular but not lobed
curled - --CORRECT ANSWER--wave pattern on margin
entire margin - --CORRECT ANSWER--even smooth all around
Filiform Growth - --CORRECT ANSWER--Agar slant. Colony exhibits a
dense/opaque surface with a defined edge.
Friable Growth - --CORRECT ANSWER--Agar slants. Colony exhibits crusty,
irregular edges.
Spreading edges - --CORRECT ANSWER--motile organism
....COPYRIGHT ©️ 2025 ALL RIGHTS RESERVED...TRUSTED & VERIFIED 3
2025 Questions and Answers
How is Media made? - --CORRECT ANSWER--5 g. T.S.A. and 125 mL Di water,
cover flask with foil, affix autoclave tape, autoclave, wait 10-20 min to cool, pour
into petri-dish to thickness of lid. or stop when covers bottom.
Agar - --CORRECT ANSWER--linear polysaccharide polymer derived from
agarose in red algae.
What does agar do? - --CORRECT ANSWER--increases solidity, exhibits
hysteresis- gels and melts at different temps. Melts above 85-90 and solidifies at 40-
45.
What is liquid media? - --CORRECT ANSWER--commonly called broths, doesn't
contain agar, easy to use, allows observation of growth patterns.
Advantages to using semi-solid media - --CORRECT ANSWER--To test oxygen
requirements, to test motility.
Fastidious - --CORRECT ANSWER--only grows in a fortified culture media under
specific conditions. The more _____________ the organism, the more specific
ingredients a medium must have. Uses defined media.
....COPYRIGHT ©️ 2025 ALL RIGHTS RESERVED...TRUSTED & VERIFIED 1
,Why use solid media? - --CORRECT ANSWER--The maintenance and growth of
stock cultures, to isolate single colonies, to ensure purity.
T.S.A. Plate - --CORRECT ANSWER--Tryptic Soy Agar most common medium
used in lab. Undefined media that contains casein digest, soybean digest, NaCl, and
agar.
What are the 4 biochemical families all living things are composed of? - --
CORRECT ANSWER--Proteins, Carbohydrates, lipids, nucleic acids
How much water does a bacteria cell consist of? - --CORRECT ANSWER--70%
Autoclave - --CORRECT ANSWER--sterilizes with steam at 121* C, pressure at
15 PSI for at least 15 min. Tested weekly to daily.
Reservoir - --CORRECT ANSWER--potential source of infection.
Normal commensal symbionts - --CORRECT ANSWER--S. pneumoniae and s.
aureus
Environmentally acquired microbes - --CORRECT ANSWER--p. aeruginosa and
b. cepacia
Magnifications of our microscopes - --CORRECT ANSWER--scanning: 4x-40,
low: 10x-100, high: 40x-400, oil immersion: 100x-1000
What is resolving power? - --CORRECT ANSWER--How close two objects can
be and still be distinguished as separate.
....COPYRIGHT ©️ 2025 ALL RIGHTS RESERVED...TRUSTED & VERIFIED 2
, What is the optimum resolution of the microscope using oil immersion? - --
CORRECT ANSWER--.2 micrometer.
How much does each microscope cost? - --CORRECT ANSWER--$1600
convex - --CORRECT ANSWER--Morphology of a domed colony
raised - --CORRECT ANSWER--flatter dome than convex
flat elevation - --CORRECT ANSWER--
crateriform - --CORRECT ANSWER--hole in convex colony
Umbonate - --CORRECT ANSWER--Morphology of a colony with a raised center
Rhizoid - --CORRECT ANSWER--Morphology of a branching colony
lobate - --CORRECT ANSWER--margin with lobes
undilated margins - --CORRECT ANSWER--irregular but not lobed
curled - --CORRECT ANSWER--wave pattern on margin
entire margin - --CORRECT ANSWER--even smooth all around
Filiform Growth - --CORRECT ANSWER--Agar slant. Colony exhibits a
dense/opaque surface with a defined edge.
Friable Growth - --CORRECT ANSWER--Agar slants. Colony exhibits crusty,
irregular edges.
Spreading edges - --CORRECT ANSWER--motile organism
....COPYRIGHT ©️ 2025 ALL RIGHTS RESERVED...TRUSTED & VERIFIED 3
