BCH 5413 EXAM 1 ACTUAL QUESTIONS
AND CORRECT DETAILED ANSWERS
(100% VERIFIED) | ALREADY GRADED A+ |
NEW UPDATE 2025-2026
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BCH 5413
,8/2/25, 8:08 PM BCH 5413 Exam 1
,8/2/25, 8:08 PM BCH 5413 Exam 1
, 8/2/25, 8:08 PM BCH 5413 Exam 1
BCH 5413 EXAM 1 ACTUAL QUESTIONS AND CORRECT DETAILED ANSWERS (100% VERIFIED) | ALREADY GRADED A+ | NEW UPDATE The number of bases per turn of DNA is___________ ~10.5 What does the Meselson and Stahl experiment confirm? DNA replication is semi-conservative Heavy and light strands with nitrogen isotopes prove True or False a. Most biological DNA is positively supercoiled. b. Triplex DNA is pyrimidine rich c. Chargaff's rule: A=G & T=C d. A single zinc finger can recognize 10bp of DNA A. False, negatively = Left-handed. Some is right handed = positive B. True C. False A=T C=G D. False, 3 bp, use multiple zinc fingers in a row for more sequence specificity/bp DNA is not a uniform structure, many localized variants can substitute protein domains, alpha helix/beta hairpin, in zinc fingers to make hybrids with different binding specificities (designated by alpha helix) for major grooves of DNA x-ray crystallography allows us to view and design custom designer DNA-binding proteins = alter gene fxn in intact cells In A & Z DNA: a. Which is right handed helix? b. Which one has 12bp per helical turn? c. Which is the dehydrated form? a. A b. Z c. A primary DNA is B Gel electrophoresis molecular separation technique can separate nucleic acids or proteins make AGAROSE gel with slots hot liquid poured with "teeth" put DNA in slot, run electric current at neutral pH DNA = negatively charged (phosphates) = migrates towards positive end key is friction DNA stained with fluorescent dye, looked at under UV unknown DNA electrophoresed parallel with known to determine size distance migrated = can plot on graph with log of molecular weights or number of bp ex: 20mm = 910bp
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