Science Biology Microbiology
BIOD 171 MODULE 3 EXAM, BIOD 171 EXAM 3
(2022)
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Straighterline Micro 250 final prep MIP 300 Micro Final Exam Po
227 terms 25 terms Teacher 40 terms
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A nanometer is defined as: 10 -9 or one-billionth of a meter
False
True or False: A nanometer is
longer than a micrometer
A nanometer is 1000x smaller than a micrometer
Resolution refers to the distance between two objects at which
the objects can still be seen as separate. Poor or low resolution
means two or more objects may appear as one.
Resolution and contrast are two Contrast on the other hand is the difference in light absorbance
critical factors that influence between two objects. Poor contrast gives a high background and
your ability to see an object. makes the visualization of multiple objects difficult. For instance,
Explain each. trying to identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the
middle of
a sunny afternoon (bright light against 2 dark objects = high
contrast).
Objective
Assuming a fixed ocular, identify
the part of the microscope you
Only the oculars (eyepiece) and the objectives contribute to the
would adjust to enhance the
magnification of the sample. Since the eyepiece
magnification of a sample.
is fixed, only the objectives could be altered.
Assuming a constant (non- Iris diaphragm
adjustable) light source power,
identify the part of the
microscope you would adjust to
limit the amount of light entering
the microscope.
, What is the total magnification 60x objective x 10x ocular = 600x magnification
on (relative to your eye) of a
sample imaged with a 60x
objective and a 10x eyepiece?
Show your math.
True
True or False: Staining is often Adherent, flat and unstained cells are almost invisible due to the
required to image a cell that is limits on both
adherent and flat (thin). resolution and contrast. Therefore, cell staining is often required
to adequately image
the sample.
True or False: A cell that is False: Adherent, flat cells are almost invisible due to the limits on
adherent, flat (thin), and both resolution and contrast
unstained is easily identified
using bright field microscopy
See an expert-written answer!
Which of the following could be B. Protozoa with diameter of 150 μm
seen clearly by the unaided D. Skin cell with diameter of 1500 μm
eye? Select all that apply.
The unaided eye can, on average, clearly resolve objects > 100
A. Bacteria with diameter of 24 μm
μm
B. Protozoa with diameter of 150
μm
C. Virus with a diameter of 0.2
μm
D. Skin cell with diameter of 1500
μm
2. Which of the following could A. Bacteria with diameter of 24 μm
NOT be seen clearly by the C. Virus with a diameter of 0.2 μm
unaided eye? Select all that
apply. The unaided eye can, on average, clearly resolve objects > 100
μm
A. Bacteria with diameter of 24
μm
B. Protozoa with diameter of 150
μm
C. Virus with a diameter of 0.2
μm
D. Skin cell with diameter of 1500
μm
1F- eyepiece
1. Label the following unmarked
2D- neck
microscope components
3B- fine adjustment knob
(numbered arrows) by matching
4G- objective
it with the components provided
5A- stage
(letters).
6H- base
This type of microscope is best Fluorescence
suited for visualizing GFP, RFP,
and YFP proteins.
BIOD 171 MODULE 3 EXAM, BIOD 171 EXAM 3
(2022)
Save
Students also studied
Flashcard sets Study guides
Straighterline Micro 250 final prep MIP 300 Micro Final Exam Po
227 terms 25 terms Teacher 40 terms
Preview Preview jacobmumo83
A nanometer is defined as: 10 -9 or one-billionth of a meter
False
True or False: A nanometer is
longer than a micrometer
A nanometer is 1000x smaller than a micrometer
Resolution refers to the distance between two objects at which
the objects can still be seen as separate. Poor or low resolution
means two or more objects may appear as one.
Resolution and contrast are two Contrast on the other hand is the difference in light absorbance
critical factors that influence between two objects. Poor contrast gives a high background and
your ability to see an object. makes the visualization of multiple objects difficult. For instance,
Explain each. trying to identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the
middle of
a sunny afternoon (bright light against 2 dark objects = high
contrast).
Objective
Assuming a fixed ocular, identify
the part of the microscope you
Only the oculars (eyepiece) and the objectives contribute to the
would adjust to enhance the
magnification of the sample. Since the eyepiece
magnification of a sample.
is fixed, only the objectives could be altered.
Assuming a constant (non- Iris diaphragm
adjustable) light source power,
identify the part of the
microscope you would adjust to
limit the amount of light entering
the microscope.
, What is the total magnification 60x objective x 10x ocular = 600x magnification
on (relative to your eye) of a
sample imaged with a 60x
objective and a 10x eyepiece?
Show your math.
True
True or False: Staining is often Adherent, flat and unstained cells are almost invisible due to the
required to image a cell that is limits on both
adherent and flat (thin). resolution and contrast. Therefore, cell staining is often required
to adequately image
the sample.
True or False: A cell that is False: Adherent, flat cells are almost invisible due to the limits on
adherent, flat (thin), and both resolution and contrast
unstained is easily identified
using bright field microscopy
See an expert-written answer!
Which of the following could be B. Protozoa with diameter of 150 μm
seen clearly by the unaided D. Skin cell with diameter of 1500 μm
eye? Select all that apply.
The unaided eye can, on average, clearly resolve objects > 100
A. Bacteria with diameter of 24 μm
μm
B. Protozoa with diameter of 150
μm
C. Virus with a diameter of 0.2
μm
D. Skin cell with diameter of 1500
μm
2. Which of the following could A. Bacteria with diameter of 24 μm
NOT be seen clearly by the C. Virus with a diameter of 0.2 μm
unaided eye? Select all that
apply. The unaided eye can, on average, clearly resolve objects > 100
μm
A. Bacteria with diameter of 24
μm
B. Protozoa with diameter of 150
μm
C. Virus with a diameter of 0.2
μm
D. Skin cell with diameter of 1500
μm
1F- eyepiece
1. Label the following unmarked
2D- neck
microscope components
3B- fine adjustment knob
(numbered arrows) by matching
4G- objective
it with the components provided
5A- stage
(letters).
6H- base
This type of microscope is best Fluorescence
suited for visualizing GFP, RFP,
and YFP proteins.
