CHEM 210 Biochemistry Module 6 Exam
(2025/2026) – Portage Learning Questions and
Verified Answers | 100% Guarantee Pass
Multiple-Choice Questions (1–20)
Question 1: Which nucleic acid is double-stranded in eukaryotic cells?
A) mRNA
B) tRNA
C) DNA
D) rRNA
Answer: C) DNA
Rationale: DNA is double-stranded, forming a double helix, while RNA types are single-
stranded in eukaryotes.
Question 2: What enzyme unwinds DNA during replication?
A) DNA polymerase
B) Helicase
C) Ligase
D) Primase
Answer: B) Helicase
Rationale: Helicase unwinds the DNA double helix, creating a replication fork for synthesis.
Question 3: Which base pairs with guanine in RNA?
A) Adenine
B) Thymine
C) Cytosine
D) Uracil
Answer: C) Cytosine
Rationale: Guanine pairs with cytosine via three hydrogen bonds in RNA, similar to DNA.
Question 4: What is the primary function of rRNA?
A) Carries amino acids
B) Forms ribosome structure
C) Carries genetic code
D) Initiates transcription
Answer: B) Forms ribosome structure
Rationale: rRNA combines with proteins to form ribosomes, essential for protein synthesis.
Question 5: What is the role of DNA polymerase in replication?
A) Unwinds DNA
B) Adds nucleotides to new strands
, C) Seals nicks in the backbone
D) Synthesizes RNA primers
Answer: B) Adds nucleotides to new strands
Rationale: DNA polymerase adds nucleotides to the growing DNA strand in the 5’ to 3’
direction.
Question 6: Which process converts DNA to RNA?
A) Translation
B) Transcription
C) Replication
D) Splicing
Answer: B) Transcription
Rationale: Transcription synthesizes RNA from a DNA template, mediated by RNA
polymerase.
Question 7: What is the start codon in translation?
A) UAG
B) AUG
C) UAA
D) UGA
Answer: B) AUG
Rationale: AUG codes for methionine and signals the start of translation.
Question 8: Which RNA type has an anticodon?
A) mRNA
B) tRNA
C) rRNA
D) snRNA
Answer: B) tRNA
Rationale: tRNA contains an anticodon that pairs with mRNA codons during translation.
Question 9: What is the primary location of DNA replication in eukaryotic cells?
A) Cytoplasm
B) Nucleus
C) Mitochondria
D) Ribosomes
Answer: B) Nucleus
Rationale: DNA replication occurs in the nucleus, where genomic DNA is stored.
Question 10: Which molecule provides energy for transcription?
A) ATP
B) GTP
C) CTP
D) UTP
Answer: A) ATP
Rationale: ATP provides energy for RNA polymerase to synthesize RNA during transcription.
(2025/2026) – Portage Learning Questions and
Verified Answers | 100% Guarantee Pass
Multiple-Choice Questions (1–20)
Question 1: Which nucleic acid is double-stranded in eukaryotic cells?
A) mRNA
B) tRNA
C) DNA
D) rRNA
Answer: C) DNA
Rationale: DNA is double-stranded, forming a double helix, while RNA types are single-
stranded in eukaryotes.
Question 2: What enzyme unwinds DNA during replication?
A) DNA polymerase
B) Helicase
C) Ligase
D) Primase
Answer: B) Helicase
Rationale: Helicase unwinds the DNA double helix, creating a replication fork for synthesis.
Question 3: Which base pairs with guanine in RNA?
A) Adenine
B) Thymine
C) Cytosine
D) Uracil
Answer: C) Cytosine
Rationale: Guanine pairs with cytosine via three hydrogen bonds in RNA, similar to DNA.
Question 4: What is the primary function of rRNA?
A) Carries amino acids
B) Forms ribosome structure
C) Carries genetic code
D) Initiates transcription
Answer: B) Forms ribosome structure
Rationale: rRNA combines with proteins to form ribosomes, essential for protein synthesis.
Question 5: What is the role of DNA polymerase in replication?
A) Unwinds DNA
B) Adds nucleotides to new strands
, C) Seals nicks in the backbone
D) Synthesizes RNA primers
Answer: B) Adds nucleotides to new strands
Rationale: DNA polymerase adds nucleotides to the growing DNA strand in the 5’ to 3’
direction.
Question 6: Which process converts DNA to RNA?
A) Translation
B) Transcription
C) Replication
D) Splicing
Answer: B) Transcription
Rationale: Transcription synthesizes RNA from a DNA template, mediated by RNA
polymerase.
Question 7: What is the start codon in translation?
A) UAG
B) AUG
C) UAA
D) UGA
Answer: B) AUG
Rationale: AUG codes for methionine and signals the start of translation.
Question 8: Which RNA type has an anticodon?
A) mRNA
B) tRNA
C) rRNA
D) snRNA
Answer: B) tRNA
Rationale: tRNA contains an anticodon that pairs with mRNA codons during translation.
Question 9: What is the primary location of DNA replication in eukaryotic cells?
A) Cytoplasm
B) Nucleus
C) Mitochondria
D) Ribosomes
Answer: B) Nucleus
Rationale: DNA replication occurs in the nucleus, where genomic DNA is stored.
Question 10: Which molecule provides energy for transcription?
A) ATP
B) GTP
C) CTP
D) UTP
Answer: A) ATP
Rationale: ATP provides energy for RNA polymerase to synthesize RNA during transcription.
