BIOCHEM final! Chem 4352 SFASU || with 100% Correct Answers.
What is the weakest intermolecular force? correct answers Van der Waals
What is delta G at equilibrium? correct answers delta G = 0
what is the first amino acid in bacterial protein synthesis correct answers fMet (fMethionine)
characteristics of a double helix correct answers Has a minor and major groove, right handed,
complementary, antiparallel
which direction does transcription occur? correct answers 5' to 3' direction
Define transcription correct answers The process of copying the genetic code from DNA into the
form of mRNA
prokaryotic promoter sites correct answers -10 region (Pribnow box), -35 region, +1 initiation
site (downstream)
eukaryotic promoter sites correct answers TATA box & many others
Where do amino acids attach to tRNA? correct answers 3' end
what type of RNA is largest correct answers mRNA (it has a complete nucleotide seq. and is
considered large)
H2SO4 correct answers sulfuric acid
,CH3OCH2CH3 correct answers ethyl methyl ether
CH3CH2CH=CHCH3 correct answers 2-pentene
Properties of water correct answers strong hydrogen bonds, high specific heat, high boiling point,
expands when freezes
What enzyme synthesizes DNA from RNA correct answers reverse transcriptase
what amino acid is usually first in translation (in eukaryotes) correct answers methionine
What form of DNA is left handed? correct answers Z form
what kind of carbon-carbon bond is shortest? correct answers triple bond (strength is inversely
related to length, short bonds are strongest)
what type of RNA contains the anticodon correct answers tRNA
what is pyrophosphate correct answers two phosphates (PPi)
what happens to pyrophosphate after it's made? correct answers it's hydrolyzed, ensures the
reaction in energetically favorable
how does transcription terminate in prokaryotes correct answers by using a rho protein or
forming a hairpin
characteristics of plasmids used in dna technology correct answers possess a gene for antibiotic
resistance, are a type of vector, replicate independently of the host genome, are circular double
stranded molecules
, Define transformation correct answers A process that places foreign DNA, such as a plasmid,
into a cell.
translation is the synthesis of protein using directions in _______ correct answers mRNA
which DNA sequencing techniques use DNA polymerase? correct answers Sanger dideoxy
method, pyrosequencing, PCR, ion semiconductor sequencing
which DNA sequencing techniques use a labeled probe? correct answers Southern Bot,
Restriction Fragment Length Polymorphism (RFLP), northern blot
what splices introns in eukaryotes correct answers the spliceosome
polylinker site correct answers short sequence in plasmids containing multiple unique restriction
enzyme recognition sites
What is a transgenic organism? correct answers An organism that has been permanently altered
by the addition of a DNA sequence to its genome.
what are two ways of disrupting or editing a gene to provide clues about the gene's function?
correct answers Zinc Finger Nucleases and TALEN
What is a bacteriophage? correct answers A virus that infects bacteria, has linear double stranded
DNA
genomic library correct answers library made by fragmenting all the DNA in an organism and
putting it in a vector
which enzymes cleave DNA at palindromic sequences? correct answers restriction enzymes
What is the weakest intermolecular force? correct answers Van der Waals
What is delta G at equilibrium? correct answers delta G = 0
what is the first amino acid in bacterial protein synthesis correct answers fMet (fMethionine)
characteristics of a double helix correct answers Has a minor and major groove, right handed,
complementary, antiparallel
which direction does transcription occur? correct answers 5' to 3' direction
Define transcription correct answers The process of copying the genetic code from DNA into the
form of mRNA
prokaryotic promoter sites correct answers -10 region (Pribnow box), -35 region, +1 initiation
site (downstream)
eukaryotic promoter sites correct answers TATA box & many others
Where do amino acids attach to tRNA? correct answers 3' end
what type of RNA is largest correct answers mRNA (it has a complete nucleotide seq. and is
considered large)
H2SO4 correct answers sulfuric acid
,CH3OCH2CH3 correct answers ethyl methyl ether
CH3CH2CH=CHCH3 correct answers 2-pentene
Properties of water correct answers strong hydrogen bonds, high specific heat, high boiling point,
expands when freezes
What enzyme synthesizes DNA from RNA correct answers reverse transcriptase
what amino acid is usually first in translation (in eukaryotes) correct answers methionine
What form of DNA is left handed? correct answers Z form
what kind of carbon-carbon bond is shortest? correct answers triple bond (strength is inversely
related to length, short bonds are strongest)
what type of RNA contains the anticodon correct answers tRNA
what is pyrophosphate correct answers two phosphates (PPi)
what happens to pyrophosphate after it's made? correct answers it's hydrolyzed, ensures the
reaction in energetically favorable
how does transcription terminate in prokaryotes correct answers by using a rho protein or
forming a hairpin
characteristics of plasmids used in dna technology correct answers possess a gene for antibiotic
resistance, are a type of vector, replicate independently of the host genome, are circular double
stranded molecules
, Define transformation correct answers A process that places foreign DNA, such as a plasmid,
into a cell.
translation is the synthesis of protein using directions in _______ correct answers mRNA
which DNA sequencing techniques use DNA polymerase? correct answers Sanger dideoxy
method, pyrosequencing, PCR, ion semiconductor sequencing
which DNA sequencing techniques use a labeled probe? correct answers Southern Bot,
Restriction Fragment Length Polymorphism (RFLP), northern blot
what splices introns in eukaryotes correct answers the spliceosome
polylinker site correct answers short sequence in plasmids containing multiple unique restriction
enzyme recognition sites
What is a transgenic organism? correct answers An organism that has been permanently altered
by the addition of a DNA sequence to its genome.
what are two ways of disrupting or editing a gene to provide clues about the gene's function?
correct answers Zinc Finger Nucleases and TALEN
What is a bacteriophage? correct answers A virus that infects bacteria, has linear double stranded
DNA
genomic library correct answers library made by fragmenting all the DNA in an organism and
putting it in a vector
which enzymes cleave DNA at palindromic sequences? correct answers restriction enzymes
