ASCP CG Certification Verified Multiple Choice and
Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
BM cyclin - a combination of two an-
What is the best agent to use against
tibiotics (tiamulin and minocycline) that
mycoplasma?
effecticely targets mycoplasma growth
For successful harvest, each new batch
comparing the new lot to a lot already in
of hypotonic solution should be evaluat-
use
ed by:
Proteolytic enzymes - break down tis-
Colagenase, pronase, and trypsin are:
sues into single-cell suspensions
When harvesting peripheral blood cul-
Colcemid - mitotic spindle poison that
tures, asceptic technique no longer mat-
halts cells at metaphase
ters following incubation with:
Samples that arrive at the cytogenetics
Logged in to the system
lab should first be:
Phytohemagglutinin (PHA) stimulates
T-cell lymphocytes
which type of cells into division?
To avoid culture failure, monitoring
reagents is crucial. When logging a cul-
Expiration dates of reagents used
ture failure, what is the MOST important
to document?
Which type of microscope is used when
cleaning a CVS (chorionic villus) sam- Dissection
ple?
Which cell type is most problematic if
Decidua (maternal tissue)
found in a CVS (chorionic villus) sample?
When looking at a slide under a phase
contrast scope and a lot of cytoplasm is
innappropriate slide drying time
still seen around the mets, what is the
most likely cause?
What will help prevent microbial contam-
ination and cross contamination of cul- Asceptic technique
tures?
, ASCP CG Certification Verified Multiple Choice and
Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
When there are not enough mets to com-
prepare additional slides from any re-
plete the study of a patient/case, what
maining sample
needs to be done next?
When looking at a dropped slide under a
phase scope and mets are very spread Increading the drying time by using warm
out and sparse, what could help fix the slides
issue?
What is true of an inverted microscope? the objectives are beneath the stage
DiGenorge syndrome -- microdeletion of
22q11, also called velocardiofacial syn-
drome
High-resolution banding is indicated for a
"velo" - soft palette,
possible diagnosis of:
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features,
short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropri-
ate for cytogenetic analysis, what is the
Reason for referral
MOST important data to accompany the
specimen?
Heparinized peripheral blood stored at
Which type of specimen should be re-
room temperature
quested for a cytogenetic analysis for
turner syndrome?
(NOT FROZEN)
A blood sample can be used to study the Dividing leukemic cells are present in cir-
cytogenetics of a malignancy only if: culation
All of the cultures in a CO2 incuba-
Temperature,
tor failed, what aspects of the incubator
CO2 level, and
are important to monitor to assess the
Humidity level
source of the problem?
The most important factor affecting the
Slide drying after the cells have been
quality of chromosomes viewed under a
dropped
phase contrast scope is:
, ASCP CG Certification Verified Multiple Choice and
Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
What is the modal number (default num-
ber of chromosomes in a cell) for a Down 45
syndrome translocation carrier?
What is the best tissue to use for both a
good success rate and abnormality de-
Chorionic villi
tection in spontaneous abortion miscar-
riages?
What is a possible cause for a drying
oven to not maintain a proper tempera- The door does not seal properly
ture?
Mycoplasma
--> the most common contaminant by a
If a tech with a cold and sneezing does
sick tech and can be detected through a
not take proper precautions while setting
specific stain used on the sample
up amniotic fluid samples, the specimen
could become contaminated with:
mycoplasma can induce abnormalities in
a sample, so it is important to avoid con-
tamination
Cell density - thru dilution of the sample
with fix
What about the metaphases on a slide Banding - based on how dark/pale/re-
are affected by slide making? fractive they are affects trypsin time
Chromosome spreading - affected by
drying time
What test(s) should be ordered on a
High resolution banding and FISH for the
newborn with suspected Prader-Willi
Prader-Willi locus on 15q11.2
syndrome?
When capturing a metaphase, a tech The camera is not parfocal (in focus with
cannot get the met into focus, what is the the microscpe objectives) with the micro-
most likely cause? scope
Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
BM cyclin - a combination of two an-
What is the best agent to use against
tibiotics (tiamulin and minocycline) that
mycoplasma?
effecticely targets mycoplasma growth
For successful harvest, each new batch
comparing the new lot to a lot already in
of hypotonic solution should be evaluat-
use
ed by:
Proteolytic enzymes - break down tis-
Colagenase, pronase, and trypsin are:
sues into single-cell suspensions
When harvesting peripheral blood cul-
Colcemid - mitotic spindle poison that
tures, asceptic technique no longer mat-
halts cells at metaphase
ters following incubation with:
Samples that arrive at the cytogenetics
Logged in to the system
lab should first be:
Phytohemagglutinin (PHA) stimulates
T-cell lymphocytes
which type of cells into division?
To avoid culture failure, monitoring
reagents is crucial. When logging a cul-
Expiration dates of reagents used
ture failure, what is the MOST important
to document?
Which type of microscope is used when
cleaning a CVS (chorionic villus) sam- Dissection
ple?
Which cell type is most problematic if
Decidua (maternal tissue)
found in a CVS (chorionic villus) sample?
When looking at a slide under a phase
contrast scope and a lot of cytoplasm is
innappropriate slide drying time
still seen around the mets, what is the
most likely cause?
What will help prevent microbial contam-
ination and cross contamination of cul- Asceptic technique
tures?
, ASCP CG Certification Verified Multiple Choice and
Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
When there are not enough mets to com-
prepare additional slides from any re-
plete the study of a patient/case, what
maining sample
needs to be done next?
When looking at a dropped slide under a
phase scope and mets are very spread Increading the drying time by using warm
out and sparse, what could help fix the slides
issue?
What is true of an inverted microscope? the objectives are beneath the stage
DiGenorge syndrome -- microdeletion of
22q11, also called velocardiofacial syn-
drome
High-resolution banding is indicated for a
"velo" - soft palette,
possible diagnosis of:
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features,
short philtrum (upper lip indent), tubular
nose, wide spread eyes, etc
When verifying if a specimen is appropri-
ate for cytogenetic analysis, what is the
Reason for referral
MOST important data to accompany the
specimen?
Heparinized peripheral blood stored at
Which type of specimen should be re-
room temperature
quested for a cytogenetic analysis for
turner syndrome?
(NOT FROZEN)
A blood sample can be used to study the Dividing leukemic cells are present in cir-
cytogenetics of a malignancy only if: culation
All of the cultures in a CO2 incuba-
Temperature,
tor failed, what aspects of the incubator
CO2 level, and
are important to monitor to assess the
Humidity level
source of the problem?
The most important factor affecting the
Slide drying after the cells have been
quality of chromosomes viewed under a
dropped
phase contrast scope is:
, ASCP CG Certification Verified Multiple Choice and
Conceptual Actual Emended Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!Current Update
What is the modal number (default num-
ber of chromosomes in a cell) for a Down 45
syndrome translocation carrier?
What is the best tissue to use for both a
good success rate and abnormality de-
Chorionic villi
tection in spontaneous abortion miscar-
riages?
What is a possible cause for a drying
oven to not maintain a proper tempera- The door does not seal properly
ture?
Mycoplasma
--> the most common contaminant by a
If a tech with a cold and sneezing does
sick tech and can be detected through a
not take proper precautions while setting
specific stain used on the sample
up amniotic fluid samples, the specimen
could become contaminated with:
mycoplasma can induce abnormalities in
a sample, so it is important to avoid con-
tamination
Cell density - thru dilution of the sample
with fix
What about the metaphases on a slide Banding - based on how dark/pale/re-
are affected by slide making? fractive they are affects trypsin time
Chromosome spreading - affected by
drying time
What test(s) should be ordered on a
High resolution banding and FISH for the
newborn with suspected Prader-Willi
Prader-Willi locus on 15q11.2
syndrome?
When capturing a metaphase, a tech The camera is not parfocal (in focus with
cannot get the met into focus, what is the the microscpe objectives) with the micro-
most likely cause? scope
