PORTAGE55LEARNING55/BIOD5517155/55Microbiology55Lab55Not
ebook
Portage55Learning55B
IO55171-55Microbiology
Lab55Notebook
Lab55Notebook55Bookmarks55(click55to55naviga
te):55Lab55155Notebook
Lab55255Notebook
Lab55355Notebook
Lab55455Notebook
Lab55555Notebook
Lab55655Notebook
Lab55755Notebook
Lab55855Notebook
Lab55955Notebook
,PORTAGE55LEARNING55/BIOD5517155/55Microbiology55Lab55Not
ebook
Lab55155Notebook
Back55to55Home55Page
Title:5 5 0155Introduction55to55Medical55Microbiology
Objective:
Cultivation55of55lab55samples55(growth55conditions)55that55are55necessary55for55carrying55out55exp
eriments55and55study55pathogens55in55a55lab55setting.
Procedure:
• Equipment55Used:
o Keep55a55sterile55environment55to55keep55people55healthy
o The55tool55for55this55is55the55Autoclave.
▪ Cleans55equipment55at55125⁰C
o Growing:55Fixed55incubator55or55a55shaker55incubator
▪ Fixed55incubator55operates55at553755C
▪ Shaker
▪ Visualization:55Microscopy
▪ Storage:55A55refrigerator55at55455C
o General55Lab55Safety:
▪ Never55eat55or55drink55in55the55lab
• Contamination55 risks
▪ Use55personal55protective55 equipment55(PPE)
,PORTAGE55LEARNING55/BIOD5517155/55Microbiology55Lab55Not
ebook
• Latex,55nitrile55(non-
latex55gloves)55thermal,55thermal55cold55gloves55(application
55dependent)
• Lab55coat
▪ Never55leave55the55lab55while55wearing55PPE55always55remove55before55lea
ving.
• Public55hallway
• Bathroom
• Cafeteria
o Lab55Notebook:55Required55for55the55course
▪ Titling55the55experiment:55First,55Last55initial55exp#55and55Title55of55the55ex
periment.
• Ex:55OH0155Keeping55a55Lab55Notebook
▪ Objective:55The55focus55of55the55experiment
• Ex:55Objective:55To55establish55an55organized55template55for55
keeping55experimental55records,55procedures,55and55results
.
▪ Procedure:55list55the55steps55to55any55procedure55in55the55lab.
• This55is55where55each55step55of55a55given55protocol55is55recorded.
• Be55sure55to55clearly55indicate55any55deviations55that55may55occ
ur55during55the55experiment.
▪ Notes:55Any55additional55information55placed55in55this55section.
▪ Results:55A55summary55of55the55outcome55of55the55experiment55
should55be55described55here.
Identification55of55samples55(biochemical55assays)55to55determine55what55kind55of55bacteria55or55
pathogen55a55patient55contracted.
• Tests55available55for55each55patho
gen:55Evaluation55of55samples55(microsc
opy)
• Visualization55and55recognition55of55key55characteristics55of55pathogens:
, PORTAGE55LEARNING55/BIOD5517155/55Microbiology55Lab55Not
ebook
Notes:
Equipment55Used:
o Keep55a55sterile55environment55to55keep55people55healthy
o The55tool55for55this55is55the55Autoclave.
▪ Cleans55equipment55at55125⁰C
▪ Uses55heat55pressure55and55steam55to55sterilize55your55environment.
▪ A55chamber55with55a55thick55door55that55will55be55closed55with55equ
ipment55or55a55sample55inside55to55sterilize.
▪ Uses55hot55steam55instead55of55hot55air55because55it55sterilizes55in55
a55matter55of55minutes55verses55several55hours.
▪ When55opening55the55chamber55after55a55cycle55is55complete,55be55su
re55to55open55slowly55and55carefully55after55it55has55depressurized55to5
5avoid55being55burned55by55the55hot55steam55that55will55be55released.
o Growing:55Fixed55incubator55or55a55shaker55incubator
▪ Fixed55incubator55operates55at553755C55that55houses55petri55dishes,55
which55holds55samples,55and55tubes55which55hold55liquid55cultures.55I
t55has55a55door55that55seals55such55to55maintain55dark55conditions55fo
r55growing.
▪ Shaker:55growth55media,55a55liquid55nutrient55broth,55is55placed55in55th
e55metal55holders55to55be55shook.55The55shaker55is55used55to55inoculate55
bacteria55as55it55rotates55the55sample55to55aerate55it55and55provide55opt
imal55conditions55for55bacteria55growth.
▪ Visualization:55Microscopy
▪ Storage:55A55refrigerator55at554O55C55is55used55to55slow55the55growth55of
55bacteria55and55preserve55samples.55Petri55dishes55are55wrapped55in55a
55para55film,55a55waxy55film55to55keep55it55airtight.
o General55Lab55Safety:
▪ Use55personal55protective55 equipment55(PPE)
• non-latex55available55usually55purple,55blue,55or55green
ebook
Portage55Learning55B
IO55171-55Microbiology
Lab55Notebook
Lab55Notebook55Bookmarks55(click55to55naviga
te):55Lab55155Notebook
Lab55255Notebook
Lab55355Notebook
Lab55455Notebook
Lab55555Notebook
Lab55655Notebook
Lab55755Notebook
Lab55855Notebook
Lab55955Notebook
,PORTAGE55LEARNING55/BIOD5517155/55Microbiology55Lab55Not
ebook
Lab55155Notebook
Back55to55Home55Page
Title:5 5 0155Introduction55to55Medical55Microbiology
Objective:
Cultivation55of55lab55samples55(growth55conditions)55that55are55necessary55for55carrying55out55exp
eriments55and55study55pathogens55in55a55lab55setting.
Procedure:
• Equipment55Used:
o Keep55a55sterile55environment55to55keep55people55healthy
o The55tool55for55this55is55the55Autoclave.
▪ Cleans55equipment55at55125⁰C
o Growing:55Fixed55incubator55or55a55shaker55incubator
▪ Fixed55incubator55operates55at553755C
▪ Shaker
▪ Visualization:55Microscopy
▪ Storage:55A55refrigerator55at55455C
o General55Lab55Safety:
▪ Never55eat55or55drink55in55the55lab
• Contamination55 risks
▪ Use55personal55protective55 equipment55(PPE)
,PORTAGE55LEARNING55/BIOD5517155/55Microbiology55Lab55Not
ebook
• Latex,55nitrile55(non-
latex55gloves)55thermal,55thermal55cold55gloves55(application
55dependent)
• Lab55coat
▪ Never55leave55the55lab55while55wearing55PPE55always55remove55before55lea
ving.
• Public55hallway
• Bathroom
• Cafeteria
o Lab55Notebook:55Required55for55the55course
▪ Titling55the55experiment:55First,55Last55initial55exp#55and55Title55of55the55ex
periment.
• Ex:55OH0155Keeping55a55Lab55Notebook
▪ Objective:55The55focus55of55the55experiment
• Ex:55Objective:55To55establish55an55organized55template55for55
keeping55experimental55records,55procedures,55and55results
.
▪ Procedure:55list55the55steps55to55any55procedure55in55the55lab.
• This55is55where55each55step55of55a55given55protocol55is55recorded.
• Be55sure55to55clearly55indicate55any55deviations55that55may55occ
ur55during55the55experiment.
▪ Notes:55Any55additional55information55placed55in55this55section.
▪ Results:55A55summary55of55the55outcome55of55the55experiment55
should55be55described55here.
Identification55of55samples55(biochemical55assays)55to55determine55what55kind55of55bacteria55or55
pathogen55a55patient55contracted.
• Tests55available55for55each55patho
gen:55Evaluation55of55samples55(microsc
opy)
• Visualization55and55recognition55of55key55characteristics55of55pathogens:
, PORTAGE55LEARNING55/BIOD5517155/55Microbiology55Lab55Not
ebook
Notes:
Equipment55Used:
o Keep55a55sterile55environment55to55keep55people55healthy
o The55tool55for55this55is55the55Autoclave.
▪ Cleans55equipment55at55125⁰C
▪ Uses55heat55pressure55and55steam55to55sterilize55your55environment.
▪ A55chamber55with55a55thick55door55that55will55be55closed55with55equ
ipment55or55a55sample55inside55to55sterilize.
▪ Uses55hot55steam55instead55of55hot55air55because55it55sterilizes55in55
a55matter55of55minutes55verses55several55hours.
▪ When55opening55the55chamber55after55a55cycle55is55complete,55be55su
re55to55open55slowly55and55carefully55after55it55has55depressurized55to5
5avoid55being55burned55by55the55hot55steam55that55will55be55released.
o Growing:55Fixed55incubator55or55a55shaker55incubator
▪ Fixed55incubator55operates55at553755C55that55houses55petri55dishes,55
which55holds55samples,55and55tubes55which55hold55liquid55cultures.55I
t55has55a55door55that55seals55such55to55maintain55dark55conditions55fo
r55growing.
▪ Shaker:55growth55media,55a55liquid55nutrient55broth,55is55placed55in55th
e55metal55holders55to55be55shook.55The55shaker55is55used55to55inoculate55
bacteria55as55it55rotates55the55sample55to55aerate55it55and55provide55opt
imal55conditions55for55bacteria55growth.
▪ Visualization:55Microscopy
▪ Storage:55A55refrigerator55at554O55C55is55used55to55slow55the55growth55of
55bacteria55and55preserve55samples.55Petri55dishes55are55wrapped55in55a
55para55film,55a55waxy55film55to55keep55it55airtight.
o General55Lab55Safety:
▪ Use55personal55protective55 equipment55(PPE)
• non-latex55available55usually55purple,55blue,55or55green
