ASCP Cancer Cytogenetics with correct answers 2025
Blast cell - correct answerimmature precursor of differentiated cells
Amount of bone marrow needed for successful analysis - correct answer1-3mL according to the
study guide, if the WBC count is low more is better
Clonal expansion - correct answerincrease in numbers of a clone of cells that have identical
characteristics
Double minutes - correct answerextrachromosomal structures that contain thousand of copies
of an amplified oncogene
Fanconi's anemia - correct answerautosomal recessive disorder characterized by bone marrow
failure caused by a defect in DNA repair, patients present aplastic anemia at early age, have
skeletal deformities and a predisposition for leukemia
Hemocytometer - correct answermanual apparatus used to estimate the # of blood cells in a
specific volume of whole blood
LPS - correct answerLipopolysaccharide (complex of lipid and carbohydrate) used to stimulate B
lymphocytes to divide
Leukemia - correct answermalignant neoplasm of the hematopoietic tissues that arises due to
clonal expansion of abnormal white blood cells
Lymphoma - correct answermalignant neoplasm of lymphoid tissue
Mitogen - correct answersubstance that stimulates division by transforming lymphocytes into
blasts
, Transformation - correct answerthe event that immortalizes cells, so they can divide
continuously w/o normal limitations
What to review for an oncology case - correct answerClonal evolution: compare previous
karyotype, document new clones
Follow-up after therapy: is clone gone, has percentage of clone reduced
Follow-up after relapse: has clone reappeared, has % of clone increased, has clonal evolution
occurred
Correlation with Flow Cytometry
Correlation with other pathology results
Cells counted for acquired abnormalities - correct answer20-30 cells
50-100 for cancer predisposition
50 for breakage studies
important to analyze cells grown under dif conditions
Reason for analysis of PB lymphocytes - correct answerLeukemia or lymphomas with circulating
immature blasts
To rule out constitutional abnormality
Family studies when a balanced translocation carrier is expected
To confirm prenatal findings in newborns (abortus tissue may also be studied to confirm
prenatal findings)
Acquired abnormalities (Clones) - correct answernon constitutional anomalies that make up a
small percentage of the body
originate from external or internal environmental forces that cause structural rearrangements
that can affect the mitotic apparatus causing numerical abnormalities
Clastogens - correct answeragents that cause chromosome breaks
Blast cell - correct answerimmature precursor of differentiated cells
Amount of bone marrow needed for successful analysis - correct answer1-3mL according to the
study guide, if the WBC count is low more is better
Clonal expansion - correct answerincrease in numbers of a clone of cells that have identical
characteristics
Double minutes - correct answerextrachromosomal structures that contain thousand of copies
of an amplified oncogene
Fanconi's anemia - correct answerautosomal recessive disorder characterized by bone marrow
failure caused by a defect in DNA repair, patients present aplastic anemia at early age, have
skeletal deformities and a predisposition for leukemia
Hemocytometer - correct answermanual apparatus used to estimate the # of blood cells in a
specific volume of whole blood
LPS - correct answerLipopolysaccharide (complex of lipid and carbohydrate) used to stimulate B
lymphocytes to divide
Leukemia - correct answermalignant neoplasm of the hematopoietic tissues that arises due to
clonal expansion of abnormal white blood cells
Lymphoma - correct answermalignant neoplasm of lymphoid tissue
Mitogen - correct answersubstance that stimulates division by transforming lymphocytes into
blasts
, Transformation - correct answerthe event that immortalizes cells, so they can divide
continuously w/o normal limitations
What to review for an oncology case - correct answerClonal evolution: compare previous
karyotype, document new clones
Follow-up after therapy: is clone gone, has percentage of clone reduced
Follow-up after relapse: has clone reappeared, has % of clone increased, has clonal evolution
occurred
Correlation with Flow Cytometry
Correlation with other pathology results
Cells counted for acquired abnormalities - correct answer20-30 cells
50-100 for cancer predisposition
50 for breakage studies
important to analyze cells grown under dif conditions
Reason for analysis of PB lymphocytes - correct answerLeukemia or lymphomas with circulating
immature blasts
To rule out constitutional abnormality
Family studies when a balanced translocation carrier is expected
To confirm prenatal findings in newborns (abortus tissue may also be studied to confirm
prenatal findings)
Acquired abnormalities (Clones) - correct answernon constitutional anomalies that make up a
small percentage of the body
originate from external or internal environmental forces that cause structural rearrangements
that can affect the mitotic apparatus causing numerical abnormalities
Clastogens - correct answeragents that cause chromosome breaks
