Risk Group 1 correct answers Low individual and low community risk.
Risk Group 2 correct answers Moderate individual risk and low community risk.
Risk Group 3 correct answers High individual risk and low community risk.
Risk Group 4 correct answers High individual risk and high community risk.
Advantages of pipetting correct answers Easily dispose of contaminated tips, accurate
measurement.
Aseptic Technique correct answers Strategies and approaches designed to create a sterile
environment.
Liquid Media correct answers Composed of a variety of ingredients that are required to support
the growth of bacteria.
Lysogeny Broth correct answers Consists of tryptone, yeast extract, and sodium chloride.
Solid media correct answers Prepared by adding agar, a thickening agent, to the ingredients used
for liquid media.
Colonies correct answers Macroscopic 3D clusters of bacterial cells in which all of the cells are
derived from a single bacterial cell.
Confluent correct answers Covered in a bacterial lawn - bacteria are too densely packed on the
agar plate for isolated colonies to grow.
,Streak plating correct answers Bacterial sample is spread out over an agar sample using an
inoculation loop, maximizing the chances of producing isolated colonies.
Generation time correct answers The length of time it takes for a cell to divide.
Temperature and bacterial growth correct answers Different kinds of bacteria grow optimally at
different temperatures, usually related to their natural habitat.
Aeration and bacterial growth correct answers Some bacteria grow best is the presence of high
oxygen levels, other bacteria are anaerobic and do not need oxygen to grow.
Lag Phase correct answers Bacteria are busy adapting to the conditions in the culture. They begin
producing enzymes to digest the food sources present in the culture growth media. The bacterial
population does not increase.
Logarithmic Phase correct answers Bacterial replication is maximized and the culture population
increases exponentially. Generation time is the smallest.
Stationary Phase correct answers Bacterial death balances out bacterial replication and so
population growth stalls due to the limited availability of nutrients once a culture reaches a
certain population.
Death Phase correct answers When the culture conditions are no longer able to support bacterial
growth, the rate at which bacteria die exceeds that of bacterial replication.
FDA recommended number of colonies for plate counting correct answers 25-250 colonies are
needed in order to accurately measure the number of bacteria in a sample.
Serial dilutions correct answers The stepwise dilution of a substance in solution.
, Turbidimetry correct answers Measurement of light scattering used to quantify the cells in
bacterial culture.
The Central Dogma of Molecular Biology correct answers Consists of 3 major processes:
replication, transcription and translation.
DNA Replication correct answers Replicated by DNA polymerase to form 2 daughter DNA
molecules with identical nucleotide sequences.
DNA Transciption correct answers Transcribed by RNA polymerase to produce RNA.
mRNA Translation correct answers The nucleotide sequence of mRNA is translated by
ribosomes into chains of amino acids called polypeptides or proteins.
Capsule correct answers Some bacteria have an additional gelatinous layer covering outside the
cell membranes, generally made up of polysaccharides. This protects bacteria from immune
cells.
Flagella correct answers Appendages involved in motility, allowing the bacteria to move around
in their environments. Long whip-like structures composed of proteins.
Chromosomal DNA correct answers The large DNA molecule containing bacterial genetic
material.
Ribosomes correct answers Present in the cytoplasm and translate mRNA into the amino acid
sequences of proteins in eukaryotes.
Cytoplasmic Membrane correct answers A bilayer composed of lipids.