MLS CHEMISTRY EXAM WITH ALL CORRECT &
VERIFIED ANSWERS|ALREADY GRADED A+|UPDATED
TO PASS
Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR
and near UV regions of the spectrum? Correct answer-Tungsten filament
Which of the following isolates light within a narrow region of the spectrum? Correct answer-
Monochromator
Which of the following is not descriptive of a photomultiplier tube? Correct answer-Cannot be
used with a chopper
Which of the following is false about a photomultiplier tube? Correct answer-Is composed of an
iron plate and a layer of selenium
Which type of photodetector employs a linear arrangement that allows it to respond to a specific
wavelength resulting in complete UV visible spectrum analysis? Correct answer-Photodiode array
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass
filter used to assess? Correct answer-Wavelength accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank? Correct answer-Correct
for color contribution of the reagents
In regard to bichromatic analysis, which of the following is false? Correct answer-Absorbance is
measured at the spectral absorbance peak for a blank and the sample using the same wavelength
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the wavelengths
that are permitted to impinge on the sample will be within what wavelength range? Correct
answer-535 to 545 nm
Which of the following formulas is an expression of the Beer Lambert law that is routinely applied to
spectrophotometric analysis? Correct answer-Au X Cs / As = Cu
In spectrophotometry, which of the following is a mathematical expression of the relationship
between absorbance and transmittance? Correct answer-A = 2 - log % T
Which of the following is not a problem inherent in turbidimetry? Correct answer-Need to
maintain a constant and specific temperature
Which of the following may be associated with reflectance spectrophotometry as it relates to the dry
reagent slide technique? Correct answer-Unabsorbed, reflected light detected by photodetector
Fluorometers are designed so that the path of the exciting light is at a right angle to the path of the
emitted light. What is the purpose of this design? Correct answer-Prevent excitation light from
reaching the detector
Which of the following represents a primary advantage of performing fluorometric over absorption
spectroscopic methods of analysis? Correct answer-Increased specificity and increased sensitivity
,Which of the following may be associated with fluorescence polarization? Correct answer-Plane
polarized light is used for sample excitation
Which of the following may be associated with bioluminescence? Correct answer-Light emission
produced due to enzymatic oxidation of a substrate
Nephelometry is based on the measurement of light that is: Correct answer-Scattered by particles
in suspension
Which of the following instruments is used in the clinical lab or in reference labs to detect beta and
gamma emissions? Correct answer-Scintillation counter
Which of the following best describes chemiluminescence? Correct answer-Chemical energy
excites electrons that emit light upon return to ground state
In assaying an analyte with a single beam atomic absorption spectrophotometer, what is the
instrument actually measuring? Correct answer-Intensity of the beam from the hollow cathode
lamp after it has passed through the analyte containing flame
What is the function of the flame in atomic absorption spectroscopy? Correct answer-Bring the
metal analyte to its ground state
Most atomic absorption spectrophotometers incorporate a beam chopper and a tuned amplifier. The
purpose of these components is to avoid errors that would be caused by: Correct answer-
Measurement of light emitted by the analyte
In potentiometry, which of the following is considered the standard electrode? Correct answer-
Hydrogen electrode
In an electrolytic cell, which of the following is the half cell where reduction takes place? Correct
answer-Cathode
Mercury covered by a layer of mercurous chloride in contact with saturated potassium chloride
solution is a description of which of the following types of electrodes? Correct answer-Calomel
When a pH sensitive glass electrode is not actively in use, in what type of solution should it be kept?
Correct answer-The medium recommended by the manufacturer
When measuring K positive with an ion selective electrode by means of a liquid ion exchange
membrane, what antibiotic will be incorporated into the membrane? Correct answer-Valinomycin
Which of the following is false about ion selective electrode analysis of sodium? Correct answer-
Membrane coated with valinomycin
What are the principles of operation for a chloride analyzer that generates silver ions as part of its
reaction mechanism? Correct answer-Amperometry and coulometry
When quantifying glucose using an amperometric glucose electrode system, which of the following is
not a component of the system? Correct answer-Hexokinase reacts with glucose
, To calibrate the pH electrode in a pH blood gas analyzer, it is necessary that: Correct answer-Two
buffer solutions of known pH be used
The measurement of CO2 in blood by means of a PCO2 electrode is dependent on the: Correct
answer-Change in pH because of increased carbonic acid in the electrolyte surrounding the
electrodes
The measurement of oxygen in blood by means of a PO2 electrode involves which of the following?
Correct answer-Direct relationship between amount of oxygen in the sample and amount of current
flowing in the measuring system
Which of the following blood gas parameters are measured directly by the blood gas analyzer
electrochemically as opposed to being calculated by the instrument? Correct answer-pH, PCO2,
PO2
Which of the following statements is false about anodic stripping voltammetry (ASV)? Correct
answer-Based on potentiometry
Which of the following methods allows for the separation of charged particles based on their rates of
migration in an electric field? Correct answer-Electrophoresis
Which of the following techniques is based on electro osmotic flow? Correct answer-Capillary
electrophoresis
Which of the following is not a type of support media used for serum protein electrophoresis?
Correct answer-Celite
In serum protein electrophoresis, when a buffer solution of pH 8.6 is used, which of the following
characterizes the proteins? Correct answer-Exhibit net negative charge
Which of the following characteristics will a protein have at its isoelectric point? Correct answer-
Net zero charge
What dye may be used for staining protein bands following electrophoresis? Correct answer-
Ponceau S
When electorphoresis is performed, holes appear in the staining pattern , giving the stained protein
band a doughnut like appearance. What is the probable cause of this problem? Correct answer-
Protein concentration was too high
What is the purpose of using ampholytes in isoelectric focusing? Correct answer-Establish a pH
gradient in the gel
Which of the following is not associated with silver stains? Correct answer-Not as sensitive as
Coomassie brilliant blue
Which of the following is not associated with isoelectric focusing? Correct answer-Migration of
proteins with net charge of zero
Which of the following is an electrophoretic technique employing a pH gradient tha separates
molecules with similar isoelectric points? Correct answer-Isoelectric focusing
VERIFIED ANSWERS|ALREADY GRADED A+|UPDATED
TO PASS
Which of the following lamps provides a continuous spectrum of radiant energy in the visible, near IR
and near UV regions of the spectrum? Correct answer-Tungsten filament
Which of the following isolates light within a narrow region of the spectrum? Correct answer-
Monochromator
Which of the following is not descriptive of a photomultiplier tube? Correct answer-Cannot be
used with a chopper
Which of the following is false about a photomultiplier tube? Correct answer-Is composed of an
iron plate and a layer of selenium
Which type of photodetector employs a linear arrangement that allows it to respond to a specific
wavelength resulting in complete UV visible spectrum analysis? Correct answer-Photodiode array
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass
filter used to assess? Correct answer-Wavelength accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank? Correct answer-Correct
for color contribution of the reagents
In regard to bichromatic analysis, which of the following is false? Correct answer-Absorbance is
measured at the spectral absorbance peak for a blank and the sample using the same wavelength
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the wavelengths
that are permitted to impinge on the sample will be within what wavelength range? Correct
answer-535 to 545 nm
Which of the following formulas is an expression of the Beer Lambert law that is routinely applied to
spectrophotometric analysis? Correct answer-Au X Cs / As = Cu
In spectrophotometry, which of the following is a mathematical expression of the relationship
between absorbance and transmittance? Correct answer-A = 2 - log % T
Which of the following is not a problem inherent in turbidimetry? Correct answer-Need to
maintain a constant and specific temperature
Which of the following may be associated with reflectance spectrophotometry as it relates to the dry
reagent slide technique? Correct answer-Unabsorbed, reflected light detected by photodetector
Fluorometers are designed so that the path of the exciting light is at a right angle to the path of the
emitted light. What is the purpose of this design? Correct answer-Prevent excitation light from
reaching the detector
Which of the following represents a primary advantage of performing fluorometric over absorption
spectroscopic methods of analysis? Correct answer-Increased specificity and increased sensitivity
,Which of the following may be associated with fluorescence polarization? Correct answer-Plane
polarized light is used for sample excitation
Which of the following may be associated with bioluminescence? Correct answer-Light emission
produced due to enzymatic oxidation of a substrate
Nephelometry is based on the measurement of light that is: Correct answer-Scattered by particles
in suspension
Which of the following instruments is used in the clinical lab or in reference labs to detect beta and
gamma emissions? Correct answer-Scintillation counter
Which of the following best describes chemiluminescence? Correct answer-Chemical energy
excites electrons that emit light upon return to ground state
In assaying an analyte with a single beam atomic absorption spectrophotometer, what is the
instrument actually measuring? Correct answer-Intensity of the beam from the hollow cathode
lamp after it has passed through the analyte containing flame
What is the function of the flame in atomic absorption spectroscopy? Correct answer-Bring the
metal analyte to its ground state
Most atomic absorption spectrophotometers incorporate a beam chopper and a tuned amplifier. The
purpose of these components is to avoid errors that would be caused by: Correct answer-
Measurement of light emitted by the analyte
In potentiometry, which of the following is considered the standard electrode? Correct answer-
Hydrogen electrode
In an electrolytic cell, which of the following is the half cell where reduction takes place? Correct
answer-Cathode
Mercury covered by a layer of mercurous chloride in contact with saturated potassium chloride
solution is a description of which of the following types of electrodes? Correct answer-Calomel
When a pH sensitive glass electrode is not actively in use, in what type of solution should it be kept?
Correct answer-The medium recommended by the manufacturer
When measuring K positive with an ion selective electrode by means of a liquid ion exchange
membrane, what antibiotic will be incorporated into the membrane? Correct answer-Valinomycin
Which of the following is false about ion selective electrode analysis of sodium? Correct answer-
Membrane coated with valinomycin
What are the principles of operation for a chloride analyzer that generates silver ions as part of its
reaction mechanism? Correct answer-Amperometry and coulometry
When quantifying glucose using an amperometric glucose electrode system, which of the following is
not a component of the system? Correct answer-Hexokinase reacts with glucose
, To calibrate the pH electrode in a pH blood gas analyzer, it is necessary that: Correct answer-Two
buffer solutions of known pH be used
The measurement of CO2 in blood by means of a PCO2 electrode is dependent on the: Correct
answer-Change in pH because of increased carbonic acid in the electrolyte surrounding the
electrodes
The measurement of oxygen in blood by means of a PO2 electrode involves which of the following?
Correct answer-Direct relationship between amount of oxygen in the sample and amount of current
flowing in the measuring system
Which of the following blood gas parameters are measured directly by the blood gas analyzer
electrochemically as opposed to being calculated by the instrument? Correct answer-pH, PCO2,
PO2
Which of the following statements is false about anodic stripping voltammetry (ASV)? Correct
answer-Based on potentiometry
Which of the following methods allows for the separation of charged particles based on their rates of
migration in an electric field? Correct answer-Electrophoresis
Which of the following techniques is based on electro osmotic flow? Correct answer-Capillary
electrophoresis
Which of the following is not a type of support media used for serum protein electrophoresis?
Correct answer-Celite
In serum protein electrophoresis, when a buffer solution of pH 8.6 is used, which of the following
characterizes the proteins? Correct answer-Exhibit net negative charge
Which of the following characteristics will a protein have at its isoelectric point? Correct answer-
Net zero charge
What dye may be used for staining protein bands following electrophoresis? Correct answer-
Ponceau S
When electorphoresis is performed, holes appear in the staining pattern , giving the stained protein
band a doughnut like appearance. What is the probable cause of this problem? Correct answer-
Protein concentration was too high
What is the purpose of using ampholytes in isoelectric focusing? Correct answer-Establish a pH
gradient in the gel
Which of the following is not associated with silver stains? Correct answer-Not as sensitive as
Coomassie brilliant blue
Which of the following is not associated with isoelectric focusing? Correct answer-Migration of
proteins with net charge of zero
Which of the following is an electrophoretic technique employing a pH gradient tha separates
molecules with similar isoelectric points? Correct answer-Isoelectric focusing
