UCF-Molec Bio 2-Final Exam Questions And
Answers
Central Dogma (1958) -
correct answer ✅DNA-Replication
RNA-Transcription
Protein-Translation
Retroviruses -
correct answer ✅RNA genome
Reverse Transcription -
correct answer ✅Done by RNA-dependent DNA polymerase
Open Reading Frame -
correct answer ✅ORF. Nucleic acid composed of consecutive
(contiguous), non-overlapping triplets (codons). Translated in
protein during translation by ribosomes. Region in mRNA that
encodes a polypeptide. Fundamental unit is a codon (three
nucleotides).
Prokaryotic polycistronic -
correct answer ✅Consists of more than one ORF. Multiple
translation start sites
,UCF-Molec Bio 2-Final Exam Questions And
Answers
Eukaryotic Polycistronic -
correct answer ✅Only one ORF
Operon -
correct answer ✅Cluster of genes under the control of a single
promoter. Rare.
Eukaryotic Regulation -
correct answer ✅Used for adaption to environment, response to
stimuli, essential for cell differentiation and development.
Spatiotemporal regulation of genes -
correct answer ✅Specific genes are activated at specific time and
location. Execution of a program at specific locations. Key for
cellular differentiation in multicellular organisms.
Transcription bubble -
correct answer ✅The region of locally unwound DNA that allows
for transcription of mRNA. Located on 3'-
Template sequence -
correct answer ✅Determines the RNA sequence. Complementary
,UCF-Molec Bio 2-Final Exam Questions And
Answers
pairing. One DNA strand acts as the template strand, the other is
the sense strand (has same sequence as RNA). ATP, CTP, GTP, and
UTP are required for synthesis. RNA chains grows in 5'-3' direction
*Uridine replaces Thymidine
RNA synthesis -
correct answer ✅Catalyzed by RNA polymerase. Transcription
begins when RNA polymerase binds to promoter. The first base pair
transcribed is the start point. RNA polymerase will continue to
synthesize until it reaches the terminator. Defines a transcription
unit. Extends from promoter to terminator.
RNA polymerase does not need: -
correct answer ✅A primer.
Coding strand (sense strand) -
correct answer ✅The DNA that has the same sequence as mRNA
and is related by the genetic code, to the protein sequence it
represents.
, UCF-Molec Bio 2-Final Exam Questions And
Answers
Antisense strand (template strand) -
correct answer ✅The DNA strand that is complementary to the
sense strand, and acts at the template synthesis for mRNA.
RNA polymerase -
correct answer ✅Enzymes that synthesize RNA using a DNA
template
Promoter -
correct answer ✅Region of DNA where RNA polymerase binds to
initiate transcription
Start point (start site) -
correct answer ✅Refers to the position of DNA corresponding to
the first base incorporated into RNA
Terminator -
correct answer ✅Sequence of DNA that causes RNA polymerase to
terminate transcription
Answers
Central Dogma (1958) -
correct answer ✅DNA-Replication
RNA-Transcription
Protein-Translation
Retroviruses -
correct answer ✅RNA genome
Reverse Transcription -
correct answer ✅Done by RNA-dependent DNA polymerase
Open Reading Frame -
correct answer ✅ORF. Nucleic acid composed of consecutive
(contiguous), non-overlapping triplets (codons). Translated in
protein during translation by ribosomes. Region in mRNA that
encodes a polypeptide. Fundamental unit is a codon (three
nucleotides).
Prokaryotic polycistronic -
correct answer ✅Consists of more than one ORF. Multiple
translation start sites
,UCF-Molec Bio 2-Final Exam Questions And
Answers
Eukaryotic Polycistronic -
correct answer ✅Only one ORF
Operon -
correct answer ✅Cluster of genes under the control of a single
promoter. Rare.
Eukaryotic Regulation -
correct answer ✅Used for adaption to environment, response to
stimuli, essential for cell differentiation and development.
Spatiotemporal regulation of genes -
correct answer ✅Specific genes are activated at specific time and
location. Execution of a program at specific locations. Key for
cellular differentiation in multicellular organisms.
Transcription bubble -
correct answer ✅The region of locally unwound DNA that allows
for transcription of mRNA. Located on 3'-
Template sequence -
correct answer ✅Determines the RNA sequence. Complementary
,UCF-Molec Bio 2-Final Exam Questions And
Answers
pairing. One DNA strand acts as the template strand, the other is
the sense strand (has same sequence as RNA). ATP, CTP, GTP, and
UTP are required for synthesis. RNA chains grows in 5'-3' direction
*Uridine replaces Thymidine
RNA synthesis -
correct answer ✅Catalyzed by RNA polymerase. Transcription
begins when RNA polymerase binds to promoter. The first base pair
transcribed is the start point. RNA polymerase will continue to
synthesize until it reaches the terminator. Defines a transcription
unit. Extends from promoter to terminator.
RNA polymerase does not need: -
correct answer ✅A primer.
Coding strand (sense strand) -
correct answer ✅The DNA that has the same sequence as mRNA
and is related by the genetic code, to the protein sequence it
represents.
, UCF-Molec Bio 2-Final Exam Questions And
Answers
Antisense strand (template strand) -
correct answer ✅The DNA strand that is complementary to the
sense strand, and acts at the template synthesis for mRNA.
RNA polymerase -
correct answer ✅Enzymes that synthesize RNA using a DNA
template
Promoter -
correct answer ✅Region of DNA where RNA polymerase binds to
initiate transcription
Start point (start site) -
correct answer ✅Refers to the position of DNA corresponding to
the first base incorporated into RNA
Terminator -
correct answer ✅Sequence of DNA that causes RNA polymerase to
terminate transcription
