1|Page
BIOC 384 EXAM 2|| QUESTIONS AND 100%
CORRECT ANSWERS ALREADY GRADED A+||
LATEST AND COMPLETE UPDATE WITH
VERIFIED SOLUTIONS GUARANTEED PASS!!!
Although most protein sequencing is now done by mass spectrometry (MS),
Edman degradation (ED) is useful if large amounts of a protein can be obtained
even if from an uncharacterized species. Answer the True/False questions
regarding methods used to sequence a polypeptide fragment: - ANSWER: False -
The N-terminal residue removed by acid treatment in ED is always a methionine.
False - Trypsin and chymotrypsin treatment often provides identical peptide
fragments for ED
True - Genomic sequences are needed to predict protein sequences by the MS
method.
True - Trypsin treatment provides useful sequence information when analyzing
data from MS.
False - Protein sequencing by ED does not require purified protein but sequencing
by MS does
X-ray crystallography (XRC) and nuclear magnetic resonance (NMR)
spectroscopy provide three
dimensional structural information about the position of atoms in a purified
protein. Name one advantage
(adv) and one disadvantage (dvg) of each technique with regard to high resolution
protein structures. - ANSWER: XRC/adv; large proteins, XRC/dvg; requires
crystals, NMR/adv; soluble protein, NMR/dvg; small proteins
, 2|Page
Protein-specific antibodies are useful experimental tools in biochemistry. Choose
the one statement below that most correctly describes these biochemical reagents. -
ANSWER: Polyclonal antibodies are a heterogeneous mixture of antibodies that
recognize epitopes on an antigen.
Match each representative protein with the most correct specific major protein
functional class. Record your answer in the order of a, b, c, d, and e, by choosing
the numbers 1, 2, 3, 4, and 5. - ANSWER: Cell signaling: G Protein-coupled
receptor
Metabolic Enzyme: Malate dehydrogenase
Genomic Caretaker: RNA polymerase
Structural Protein: Tubulin
Transport Protein: Na+/K+ ATPase
Which statement most accurately describes the structure and function of O2
transport proteins. - ANSWER: Hemoglobin is a tetramer that can bind BPG and
O2 whereas myoglobin binds O2 but cannot bind BPG.
Answer the following questions about the graph shown at the right.
1. The protein with the lowest dissociation constant (Kd) is protein (A or B)?
2. The protein with the highest affinity for oxygen is protein (A or B)?
3. The protein most likely to bind oxygen cooperatively is protein (A or B)?
4. The protein most likely to be an oxygen storage protein is protein (A or B)?
BIOC 384 EXAM 2|| QUESTIONS AND 100%
CORRECT ANSWERS ALREADY GRADED A+||
LATEST AND COMPLETE UPDATE WITH
VERIFIED SOLUTIONS GUARANTEED PASS!!!
Although most protein sequencing is now done by mass spectrometry (MS),
Edman degradation (ED) is useful if large amounts of a protein can be obtained
even if from an uncharacterized species. Answer the True/False questions
regarding methods used to sequence a polypeptide fragment: - ANSWER: False -
The N-terminal residue removed by acid treatment in ED is always a methionine.
False - Trypsin and chymotrypsin treatment often provides identical peptide
fragments for ED
True - Genomic sequences are needed to predict protein sequences by the MS
method.
True - Trypsin treatment provides useful sequence information when analyzing
data from MS.
False - Protein sequencing by ED does not require purified protein but sequencing
by MS does
X-ray crystallography (XRC) and nuclear magnetic resonance (NMR)
spectroscopy provide three
dimensional structural information about the position of atoms in a purified
protein. Name one advantage
(adv) and one disadvantage (dvg) of each technique with regard to high resolution
protein structures. - ANSWER: XRC/adv; large proteins, XRC/dvg; requires
crystals, NMR/adv; soluble protein, NMR/dvg; small proteins
, 2|Page
Protein-specific antibodies are useful experimental tools in biochemistry. Choose
the one statement below that most correctly describes these biochemical reagents. -
ANSWER: Polyclonal antibodies are a heterogeneous mixture of antibodies that
recognize epitopes on an antigen.
Match each representative protein with the most correct specific major protein
functional class. Record your answer in the order of a, b, c, d, and e, by choosing
the numbers 1, 2, 3, 4, and 5. - ANSWER: Cell signaling: G Protein-coupled
receptor
Metabolic Enzyme: Malate dehydrogenase
Genomic Caretaker: RNA polymerase
Structural Protein: Tubulin
Transport Protein: Na+/K+ ATPase
Which statement most accurately describes the structure and function of O2
transport proteins. - ANSWER: Hemoglobin is a tetramer that can bind BPG and
O2 whereas myoglobin binds O2 but cannot bind BPG.
Answer the following questions about the graph shown at the right.
1. The protein with the lowest dissociation constant (Kd) is protein (A or B)?
2. The protein with the highest affinity for oxygen is protein (A or B)?
3. The protein most likely to bind oxygen cooperatively is protein (A or B)?
4. The protein most likely to be an oxygen storage protein is protein (A or B)?
