GUIDE
FOR THE CARE AND USE OF
LABORATORY
ANIMALS
Eighth Edition
CommitteeJforJtheJUpdateJofJtheJGuideJforJtheJCareJandJ
UseJofJLaboratoryJAnimals
InstituteJforJLaboratoryJAnimalJResearchJDivision
J onJEarthJandJLifeJStudies
,THEJNATIONALJACADEMIESJPRESSJ J 500JFifthJStreet,JNWJ J Washington,JDCJ20001
NOTICE:JTheJprojectJthatJisJtheJsubjectJofJthisJreportJwasJapprovedJbyJtheJGovern-
JingJBoardJofJtheJNationalJResearchJCouncil,JwhoseJmembersJareJdrawnJfromJtheJ
councilsJofJtheJNationalJAcademyJofJSciences,JtheJNationalJAcademyJofJEngineer-
Jing,JandJtheJInstituteJofJMedicine.JTheJmembersJofJtheJCommitteeJresponsibleJforJ
theJreportJwereJchosenJforJtheirJspecialJcompetencesJandJwithJregardJforJappropriateJ
balance.
ThisJstudyJwasJsupportedJbyJtheJOfficeJofJExtramuralJResearch,JOfficeJofJtheJDirec-
Jtor,JNationalJInstitutesJofJHealth/DepartmentJofJHealthJandJHumanJServicesJunderJ
ContractJNumberJN01-OD-4-
2139JTaskJOrderJ#188;JtheJOfficeJofJResearchJIntegrity,JDepartmentJofJHealthJandJH
umanJServices;JtheJAnimalJandJPlantJHealthJInspectionJService,JU.S.JDepartmentJofJA
griculture;JAssociationJforJAssessmentJandJAccreditationJofJLaboratoryJAnimalJCareJInt
ernational;JAmericanJAssociationJforJLaboratoryJAnimalJScience;JAbbottJFund;JPfizer;JA
mericanJCollegeJofJLaboratoryJAnimalJMedicine;JAmeri-
JcanJSocietyJofJLaboratoryJAnimalJPractitioners;JAssociationJofJPrimateJVeternarians.
AnyJopinions,Jfindings,Jconclusions,JorJrecommendationsJexpressedJinJthisJpub-
JlicationJareJthoseJofJtheJauthorsJandJdoJnotJnecessarilyJreflectJtheJviewsJofJtheJ
organizationsJorJagenciesJthatJprovidedJsupportJforJtheJproject.JTheJcontentJofJt
hisJpublicationJdoesJnotJnecessarilyJreflectJtheJviewsJorJpoliciesJofJtheJNationalJI
nstitutesJofJHealth,JnorJdoesJmentionJofJtradeJnames,JcommercialJproducts,JorJo
rganizationsJimplyJendorsementJbyJtheJUSJgovernment.
InternationalJStandardJBookJNumber-13:J 978-0-309-15400-
0J(Book)JInternationalJStandardJBookJNumber-10:J 0-309-15400-
6J(Book)JInternationalJStandardJBookJNumber-13:J 978-0-309-
15401-7J(PDF)JInternationalJStandardJBookJNumber-10:J 0-309-
15401-4J(PDF)JLibraryJofJCongressJControlJNumber:J 2010940400
AdditionalJcopiesJofJthisJreportJareJavailableJfromJtheJNationalJAcademiesJPress,J
500J FifthJ Street,J NW,J LockboxJ 285,J Washington,J DCJ 20055;J (800)J 624-6242J or
(202)J334-3313J(inJtheJWashingtonJmetropolitanJarea);Jhttp://www.nap.edu.
CopyrightJ2011JbyJtheJNationalJAcademyJofJSciences.JAllJrightsJreserved.J
PrintedJinJtheJUnitedJStatesJofJAmerica.
,TheJNationalJAcademyJofJSciencesJisJaJprivate,Jnonprofit,Jself-
perpetuatingJsocietyJofJdistinguishedJscholarsJengagedJinJscientificJandJengineeringJ
research,JdedicatedJtoJtheJfurtheranceJofJscienceJandJtechnologyJandJtoJtheirJuseJforJ
theJgeneralJwelfare.JUponJtheJauthorityJofJtheJcharterJgrantedJtoJitJbyJtheJCongress
JinJ1863,JtheJAcad-
JemyJhasJaJmandateJthatJrequiresJitJtoJadviseJtheJfederalJgovernmentJonJscientificJ
andJtechnicalJmatters.JDr.JRalphJJ.JCiceroneJisJpresidentJofJtheJNationalJAcademyJ
ofJSciences.
TheJNationalJAcademyJofJEngineeringJwasJestablishedJinJ1964,JunderJtheJcharterJ
ofJtheJNationalJAcademyJofJSciences,JasJaJparallelJorganizationJofJoutstandingJe
ngineers.JItJisJautonomousJinJitsJadministrationJandJinJtheJselectionJofJitsJmembers,Js
haringJwithJtheJNationalJAcademyJofJSciencesJtheJresponsibilityJforJadvisingJtheJf
ederalJgovernment.JTheJNationalJAcademyJofJEngineeringJalsoJsponsorsJengineer-
JingJprogramsJaimedJatJmeetingJnationalJneeds,JencouragesJeducationJandJresearch,J
andJrecognizesJtheJsuperiorJachievementsJofJengineers.JDr.JCharlesJVestJisJpresidentJ
ofJtheJNationalJAcademyJofJEngineering.
TheJInstituteJofJMedicineJwasJestablishedJinJ1970JbyJtheJNationalJAcademyJofJS
ciencesJtoJsecureJtheJservicesJofJeminentJmembersJofJappropriateJprofessionsJinJt
heJexaminationJofJpolicyJmattersJpertainingJtoJtheJhealthJofJtheJpublic.JTheJInsti-
JtuteJactsJunderJtheJresponsibilityJgivenJtoJtheJNationalJAcademyJofJSciencesJbyJitsJ
congressionalJcharterJtoJbeJanJadviserJtoJtheJfederalJgovernmentJand,JuponJitsJownJi
nitiative,JtoJidentifyJissuesJofJmedicalJcare,Jresearch,JandJeducation.JDr.JHarveyJV.JF
inebergJisJpresidentJofJtheJInstituteJofJMedicine.
TheJNationalJResearchJCouncilJwasJorganizedJbyJtheJNationalJAcademyJofJSci-
JencesJinJ1916JtoJassociateJtheJbroadJcommunityJofJscienceJandJtechnologyJwithJtheJ
Academy’sJpurposesJofJfurtheringJknowledgeJandJadvisingJtheJfederalJgovernment.J
FunctioningJinJaccordanceJwithJgeneralJpoliciesJdeterminedJbyJtheJAcademy,JtheJ
CouncilJhasJbecomeJtheJprincipalJoperatingJagencyJofJbothJtheJNationalJAcademyJ
ofJSciencesJandJtheJNationalJAcademyJofJEngineeringJinJprovidingJservicesJtoJthe
Jgovernment,JtheJpublic,JandJtheJscientificJandJengineeringJcommunities.JTheJCou
ncilJisJadministeredJjointlyJbyJbothJAcademiesJandJtheJInstituteJofJMedicine.JDr.J
RalphJJ.JCiceroneJandJDr.JCharlesJM.JVestJareJchairJandJviceJchair,Jrespectively,JofJt
heJNationalJResearchJCouncil.
www.national-academies.org
,
FOR THE CARE AND USE OF
LABORATORY
ANIMALS
Eighth Edition
CommitteeJforJtheJUpdateJofJtheJGuideJforJtheJCareJandJ
UseJofJLaboratoryJAnimals
InstituteJforJLaboratoryJAnimalJResearchJDivision
J onJEarthJandJLifeJStudies
,THEJNATIONALJACADEMIESJPRESSJ J 500JFifthJStreet,JNWJ J Washington,JDCJ20001
NOTICE:JTheJprojectJthatJisJtheJsubjectJofJthisJreportJwasJapprovedJbyJtheJGovern-
JingJBoardJofJtheJNationalJResearchJCouncil,JwhoseJmembersJareJdrawnJfromJtheJ
councilsJofJtheJNationalJAcademyJofJSciences,JtheJNationalJAcademyJofJEngineer-
Jing,JandJtheJInstituteJofJMedicine.JTheJmembersJofJtheJCommitteeJresponsibleJforJ
theJreportJwereJchosenJforJtheirJspecialJcompetencesJandJwithJregardJforJappropriateJ
balance.
ThisJstudyJwasJsupportedJbyJtheJOfficeJofJExtramuralJResearch,JOfficeJofJtheJDirec-
Jtor,JNationalJInstitutesJofJHealth/DepartmentJofJHealthJandJHumanJServicesJunderJ
ContractJNumberJN01-OD-4-
2139JTaskJOrderJ#188;JtheJOfficeJofJResearchJIntegrity,JDepartmentJofJHealthJandJH
umanJServices;JtheJAnimalJandJPlantJHealthJInspectionJService,JU.S.JDepartmentJofJA
griculture;JAssociationJforJAssessmentJandJAccreditationJofJLaboratoryJAnimalJCareJInt
ernational;JAmericanJAssociationJforJLaboratoryJAnimalJScience;JAbbottJFund;JPfizer;JA
mericanJCollegeJofJLaboratoryJAnimalJMedicine;JAmeri-
JcanJSocietyJofJLaboratoryJAnimalJPractitioners;JAssociationJofJPrimateJVeternarians.
AnyJopinions,Jfindings,Jconclusions,JorJrecommendationsJexpressedJinJthisJpub-
JlicationJareJthoseJofJtheJauthorsJandJdoJnotJnecessarilyJreflectJtheJviewsJofJtheJ
organizationsJorJagenciesJthatJprovidedJsupportJforJtheJproject.JTheJcontentJofJt
hisJpublicationJdoesJnotJnecessarilyJreflectJtheJviewsJorJpoliciesJofJtheJNationalJI
nstitutesJofJHealth,JnorJdoesJmentionJofJtradeJnames,JcommercialJproducts,JorJo
rganizationsJimplyJendorsementJbyJtheJUSJgovernment.
InternationalJStandardJBookJNumber-13:J 978-0-309-15400-
0J(Book)JInternationalJStandardJBookJNumber-10:J 0-309-15400-
6J(Book)JInternationalJStandardJBookJNumber-13:J 978-0-309-
15401-7J(PDF)JInternationalJStandardJBookJNumber-10:J 0-309-
15401-4J(PDF)JLibraryJofJCongressJControlJNumber:J 2010940400
AdditionalJcopiesJofJthisJreportJareJavailableJfromJtheJNationalJAcademiesJPress,J
500J FifthJ Street,J NW,J LockboxJ 285,J Washington,J DCJ 20055;J (800)J 624-6242J or
(202)J334-3313J(inJtheJWashingtonJmetropolitanJarea);Jhttp://www.nap.edu.
CopyrightJ2011JbyJtheJNationalJAcademyJofJSciences.JAllJrightsJreserved.J
PrintedJinJtheJUnitedJStatesJofJAmerica.
,TheJNationalJAcademyJofJSciencesJisJaJprivate,Jnonprofit,Jself-
perpetuatingJsocietyJofJdistinguishedJscholarsJengagedJinJscientificJandJengineeringJ
research,JdedicatedJtoJtheJfurtheranceJofJscienceJandJtechnologyJandJtoJtheirJuseJforJ
theJgeneralJwelfare.JUponJtheJauthorityJofJtheJcharterJgrantedJtoJitJbyJtheJCongress
JinJ1863,JtheJAcad-
JemyJhasJaJmandateJthatJrequiresJitJtoJadviseJtheJfederalJgovernmentJonJscientificJ
andJtechnicalJmatters.JDr.JRalphJJ.JCiceroneJisJpresidentJofJtheJNationalJAcademyJ
ofJSciences.
TheJNationalJAcademyJofJEngineeringJwasJestablishedJinJ1964,JunderJtheJcharterJ
ofJtheJNationalJAcademyJofJSciences,JasJaJparallelJorganizationJofJoutstandingJe
ngineers.JItJisJautonomousJinJitsJadministrationJandJinJtheJselectionJofJitsJmembers,Js
haringJwithJtheJNationalJAcademyJofJSciencesJtheJresponsibilityJforJadvisingJtheJf
ederalJgovernment.JTheJNationalJAcademyJofJEngineeringJalsoJsponsorsJengineer-
JingJprogramsJaimedJatJmeetingJnationalJneeds,JencouragesJeducationJandJresearch,J
andJrecognizesJtheJsuperiorJachievementsJofJengineers.JDr.JCharlesJVestJisJpresidentJ
ofJtheJNationalJAcademyJofJEngineering.
TheJInstituteJofJMedicineJwasJestablishedJinJ1970JbyJtheJNationalJAcademyJofJS
ciencesJtoJsecureJtheJservicesJofJeminentJmembersJofJappropriateJprofessionsJinJt
heJexaminationJofJpolicyJmattersJpertainingJtoJtheJhealthJofJtheJpublic.JTheJInsti-
JtuteJactsJunderJtheJresponsibilityJgivenJtoJtheJNationalJAcademyJofJSciencesJbyJitsJ
congressionalJcharterJtoJbeJanJadviserJtoJtheJfederalJgovernmentJand,JuponJitsJownJi
nitiative,JtoJidentifyJissuesJofJmedicalJcare,Jresearch,JandJeducation.JDr.JHarveyJV.JF
inebergJisJpresidentJofJtheJInstituteJofJMedicine.
TheJNationalJResearchJCouncilJwasJorganizedJbyJtheJNationalJAcademyJofJSci-
JencesJinJ1916JtoJassociateJtheJbroadJcommunityJofJscienceJandJtechnologyJwithJtheJ
Academy’sJpurposesJofJfurtheringJknowledgeJandJadvisingJtheJfederalJgovernment.J
FunctioningJinJaccordanceJwithJgeneralJpoliciesJdeterminedJbyJtheJAcademy,JtheJ
CouncilJhasJbecomeJtheJprincipalJoperatingJagencyJofJbothJtheJNationalJAcademyJ
ofJSciencesJandJtheJNationalJAcademyJofJEngineeringJinJprovidingJservicesJtoJthe
Jgovernment,JtheJpublic,JandJtheJscientificJandJengineeringJcommunities.JTheJCou
ncilJisJadministeredJjointlyJbyJbothJAcademiesJandJtheJInstituteJofJMedicine.JDr.J
RalphJJ.JCiceroneJandJDr.JCharlesJM.JVestJareJchairJandJviceJchair,Jrespectively,JofJt
heJNationalJResearchJCouncil.
www.national-academies.org
,
