Lab 4 Diffusion and BIO201
L
Osmosis
Student Name:
Access Code (located on the lid of your lab kit):
Lab Report Format Expectations
Utilize college level grammar and professional formaṄng when completing this worksheet.
Submissions without proper formaṄng, all required photos or sufficient responses will be rejected. Pre-
lab Questions
1. Define the following terms.
a. hypertonic solution:
A solution that has a higher concentration of solutes than another solution. Found
typically in a cell or body fluid.
b. hypotonic solution:
Solution that has a lower concentration of solutes compared to another solution. Found
in internal cellular fluid or blood.
c. isotonic solution:
Solution that has the same concentration of solutes as cells and blood preventing cells
from shrinking or swelling
2. Define osmotic pressure.
Pressure required to stop the flow of solent across a semipermeable membrane
3. Explain what would happen to a cell placed in a hypertonic
solution. The cell will shrink as water moves out of the cell
, Lab 4 Diffusion and BIO201
L
Osmosis
EXPERIMENT 1: DIFFUSION THROUGH A LIQUID
Introduction Questions
1. State the molecular weight of the blue dye in grams per mole.
792.84 g/mol
2. State the molecular weight of the red dye in grams per mole.
496.42 g/mol
3. Why does this experiment require two dyes of different molecular weights? Answer this
question, ensuring you explain the purpose of these two dyes in relation to the
experiment.
to demonstrate the separation of molecules based on size. The larger dye is faster, smaller dye is
slower.
L
Osmosis
Student Name:
Access Code (located on the lid of your lab kit):
Lab Report Format Expectations
Utilize college level grammar and professional formaṄng when completing this worksheet.
Submissions without proper formaṄng, all required photos or sufficient responses will be rejected. Pre-
lab Questions
1. Define the following terms.
a. hypertonic solution:
A solution that has a higher concentration of solutes than another solution. Found
typically in a cell or body fluid.
b. hypotonic solution:
Solution that has a lower concentration of solutes compared to another solution. Found
in internal cellular fluid or blood.
c. isotonic solution:
Solution that has the same concentration of solutes as cells and blood preventing cells
from shrinking or swelling
2. Define osmotic pressure.
Pressure required to stop the flow of solent across a semipermeable membrane
3. Explain what would happen to a cell placed in a hypertonic
solution. The cell will shrink as water moves out of the cell
, Lab 4 Diffusion and BIO201
L
Osmosis
EXPERIMENT 1: DIFFUSION THROUGH A LIQUID
Introduction Questions
1. State the molecular weight of the blue dye in grams per mole.
792.84 g/mol
2. State the molecular weight of the red dye in grams per mole.
496.42 g/mol
3. Why does this experiment require two dyes of different molecular weights? Answer this
question, ensuring you explain the purpose of these two dyes in relation to the
experiment.
to demonstrate the separation of molecules based on size. The larger dye is faster, smaller dye is
slower.
