BCHM 4360 EXAM 3 ACTUAL EXAM 2025 | ALL QUESTIONS AND
BCHM 4360 Exam 3
CORRECT ANSWERS | VERIFIED ANSWERS | ALREADY GRADED A+
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LATEST EXAM
1. What are the three 1. Contribution to regulation of gene activity
main benefits to RNA 2. Diversity - many different RNAs can be produced from one gene via
processing? alternative splicing
3. Quality control
2. Many of the RNA pro- protein, RNA, RNPs
cessing complexes
contain both _______
and ___ components.
Give an example.
3. An E. coli precur- 3, several
sor encodes __ rRNAs
and __ tRNAs.
4. The S. cerevisiae pre- 3
cursor encodes __
rRNAs.
5. Why is encoding sev- ensures that similar amounts of each RNA are made
eral RNAs in one pre-
cursor beneficial?
6. True or False: Ex- False, go both ways
onucleases operate
strictly in the 3' to 5'
direction during RNA
processing.
7. Give two examples of RNase III and RNase P
endonucleases.
8. RNase P
, BCHM 4360 Exam 3
Study online at https://quizlet.com/_hd0qzx
5' trimming of tRNAs
is done by which en-
donuclease?
9. True or False: Both False: Only bacterial can cut alone
the bacterial and eu-
karyotic RNA compo-
nent of RNase P can
cut RNA alone
10. True or False: Some True
rRNA introns can
catalyze their own
removal and are
subsequently called
self-splicing.
11. What is the CCA se- It is the attachment site on the tRNA for the amino acid
quence?
12. What are the two 1. ribose 2'-O-methylation
most common rRNA 2. pseudouridylation
modifications?
13. True or False: Modifications can be small, like methylation, but can also be large, like
False: Post-transcrip- the addition of a threonine
tional chemical mod-
ifications are limit-
ed to small additions
such as methylation
14. small nucleolar RNAs typically made from introns of precursor mRNAs and
they are typically used as guide RNAs
, BCHM 4360 Exam 3
Study online at https://quizlet.com/_hd0qzx
What are snoRNAs
and what is their
general purpose?
15. Describe how snoR- The snoRNA base-pairs with specific regions of target RNAs and directs
NAs catalyze methy- enzymes to these positions
lation of nucleotides
16. snoRNAs that direct box C/D, H/ACA
ribose methylation
are _______ snoRNAs.
snoRNAs that di-
rect pseudouridyla-
tion are ______ snoR-
NAs.
17. What is the primary protection of mRNAs from nuclease degradation and assisting with protein
purpose of the end interactions
modifications of eu-
karyotic RNAs follow-
ing transcription?
18. What are the 5' ends 7-methylguanine nucleotide via a 5'-5' triphosphate linkage
of mRNAs capped
with?
19. What is the 5' cap efficient elongation and termination of the transcript, for mRNA processing
needed for? and export from the nucleus, and for directing translation
20. What are the three 1. An RNA 5' triphosphate catalyzes removal of a phosphate from the 5' end
steps for the addi- 2. A guanyl transferase attaches a guanosine monophosphate (GMP) to the
tion of the 5' cap? end in a 5'-5' triphosphate linkage
3. The guanine is methylated by a guanine-7-methyltransferase
, BCHM 4360 Exam 3
Study online at https://quizlet.com/_hd0qzx
21. The 3' end of 200
most eukaryotic mR-
NAs have about ___
adenosines added,
known as the poly(A)
tail.
22. Polyadenylation at distal, proximal
the _______ site re-
tains multiple reg-
ulatory sequences.
Polyadenylation at
the _______ site elim-
inates the regulatory
sequences.
23. What are the steps 1. Initial cleavage after a CA that lies between a conserved AAUAAA hexamer
for the addition of and a U or GU-rich region
the poly(A) tail? 2. After cleavage, ~200 adenosines are added by poly(A) polymerase
24. What is responsible The CTD of the largest subunit of RNA Pol II, RBP1
for mediating mRNA
processing?
25. __________ is needed Capping, polyadenylation
to allow RNA Pol
II to continue tran-
scription. __________
is needed for ef-
ficient transcription
termination.
26.
BCHM 4360 Exam 3
CORRECT ANSWERS | VERIFIED ANSWERS | ALREADY GRADED A+
Study online at https://quizlet.com/_hd0qzx
LATEST EXAM
1. What are the three 1. Contribution to regulation of gene activity
main benefits to RNA 2. Diversity - many different RNAs can be produced from one gene via
processing? alternative splicing
3. Quality control
2. Many of the RNA pro- protein, RNA, RNPs
cessing complexes
contain both _______
and ___ components.
Give an example.
3. An E. coli precur- 3, several
sor encodes __ rRNAs
and __ tRNAs.
4. The S. cerevisiae pre- 3
cursor encodes __
rRNAs.
5. Why is encoding sev- ensures that similar amounts of each RNA are made
eral RNAs in one pre-
cursor beneficial?
6. True or False: Ex- False, go both ways
onucleases operate
strictly in the 3' to 5'
direction during RNA
processing.
7. Give two examples of RNase III and RNase P
endonucleases.
8. RNase P
, BCHM 4360 Exam 3
Study online at https://quizlet.com/_hd0qzx
5' trimming of tRNAs
is done by which en-
donuclease?
9. True or False: Both False: Only bacterial can cut alone
the bacterial and eu-
karyotic RNA compo-
nent of RNase P can
cut RNA alone
10. True or False: Some True
rRNA introns can
catalyze their own
removal and are
subsequently called
self-splicing.
11. What is the CCA se- It is the attachment site on the tRNA for the amino acid
quence?
12. What are the two 1. ribose 2'-O-methylation
most common rRNA 2. pseudouridylation
modifications?
13. True or False: Modifications can be small, like methylation, but can also be large, like
False: Post-transcrip- the addition of a threonine
tional chemical mod-
ifications are limit-
ed to small additions
such as methylation
14. small nucleolar RNAs typically made from introns of precursor mRNAs and
they are typically used as guide RNAs
, BCHM 4360 Exam 3
Study online at https://quizlet.com/_hd0qzx
What are snoRNAs
and what is their
general purpose?
15. Describe how snoR- The snoRNA base-pairs with specific regions of target RNAs and directs
NAs catalyze methy- enzymes to these positions
lation of nucleotides
16. snoRNAs that direct box C/D, H/ACA
ribose methylation
are _______ snoRNAs.
snoRNAs that di-
rect pseudouridyla-
tion are ______ snoR-
NAs.
17. What is the primary protection of mRNAs from nuclease degradation and assisting with protein
purpose of the end interactions
modifications of eu-
karyotic RNAs follow-
ing transcription?
18. What are the 5' ends 7-methylguanine nucleotide via a 5'-5' triphosphate linkage
of mRNAs capped
with?
19. What is the 5' cap efficient elongation and termination of the transcript, for mRNA processing
needed for? and export from the nucleus, and for directing translation
20. What are the three 1. An RNA 5' triphosphate catalyzes removal of a phosphate from the 5' end
steps for the addi- 2. A guanyl transferase attaches a guanosine monophosphate (GMP) to the
tion of the 5' cap? end in a 5'-5' triphosphate linkage
3. The guanine is methylated by a guanine-7-methyltransferase
, BCHM 4360 Exam 3
Study online at https://quizlet.com/_hd0qzx
21. The 3' end of 200
most eukaryotic mR-
NAs have about ___
adenosines added,
known as the poly(A)
tail.
22. Polyadenylation at distal, proximal
the _______ site re-
tains multiple reg-
ulatory sequences.
Polyadenylation at
the _______ site elim-
inates the regulatory
sequences.
23. What are the steps 1. Initial cleavage after a CA that lies between a conserved AAUAAA hexamer
for the addition of and a U or GU-rich region
the poly(A) tail? 2. After cleavage, ~200 adenosines are added by poly(A) polymerase
24. What is responsible The CTD of the largest subunit of RNA Pol II, RBP1
for mediating mRNA
processing?
25. __________ is needed Capping, polyadenylation
to allow RNA Pol
II to continue tran-
scription. __________
is needed for ef-
ficient transcription
termination.
26.
