BIOD 171 MODULE 3 EXAM QUESTIONS AND
CORRECT DETAILED ANSWERS WELL VERIFIED
.GRADED A+
What is the total magnification on (relative to your eye) of a sample
imaged with a 60x objective and a 10x eyepiece? Show your math. -
ANSWER -60x objective x 10x ocular = 600x magnification
True or False: Staining is often required to image a cell that is adherent
and flat (thin). - ANSWER -True
A nanometer is defined as: - ANSWER -10 -9 or one-billionth of a meter
, True or False: A nanometer is longer than a micrometer - ANSWER -
False
A nanometer is 1000x smaller than a micrometer
Resolution and contrast are two critical factors that influence your
ability to see an object. Explain each. - ANSWER -Resolution refers to
the distance between two objects at which the objects can still be seen
as separate. Poor or low resolution means two or more objects may
appear as one.
Contrast on the other hand is the difference in light absorbance
between two objects. Poor contrast gives a high background and makes
the visualization of multiple objects difficult. For instance, trying to
identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the middle
of
a sunny afternoon (bright light against 2 dark objects = high contrast).
Assuming a fixed ocular, identify the part of the microscope you would
adjust to enhance the magnification of a sample. - ANSWER -Objective
Only the oculars (eyepiece) and the objectives contribute to the
magnification of the sample. Since the eyepiece
CORRECT DETAILED ANSWERS WELL VERIFIED
.GRADED A+
What is the total magnification on (relative to your eye) of a sample
imaged with a 60x objective and a 10x eyepiece? Show your math. -
ANSWER -60x objective x 10x ocular = 600x magnification
True or False: Staining is often required to image a cell that is adherent
and flat (thin). - ANSWER -True
A nanometer is defined as: - ANSWER -10 -9 or one-billionth of a meter
, True or False: A nanometer is longer than a micrometer - ANSWER -
False
A nanometer is 1000x smaller than a micrometer
Resolution and contrast are two critical factors that influence your
ability to see an object. Explain each. - ANSWER -Resolution refers to
the distance between two objects at which the objects can still be seen
as separate. Poor or low resolution means two or more objects may
appear as one.
Contrast on the other hand is the difference in light absorbance
between two objects. Poor contrast gives a high background and makes
the visualization of multiple objects difficult. For instance, trying to
identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the middle
of
a sunny afternoon (bright light against 2 dark objects = high contrast).
Assuming a fixed ocular, identify the part of the microscope you would
adjust to enhance the magnification of a sample. - ANSWER -Objective
Only the oculars (eyepiece) and the objectives contribute to the
magnification of the sample. Since the eyepiece
