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Which of the following is an example of semi-quantitative test result? - 🧠
ANSWER ✔✔3+
Which of the following signals is light based? - 🧠 ANSWER ✔✔scatter
Which of the following under-estimates the concentration but not over-
estimates? - 🧠 ANSWER ✔✔downward shift
What is the purpose of the reagent blank in spectrophotometric analysis? -
🧠 ANSWER ✔✔to subtract the color contribution of the reagent
Which of the following describes a specimen blank? - 🧠 ANSWER
✔✔subtract the light absorption of patient serum.
Which of the following statement is true about lower limit of detection and
reagent blank? - 🧠 ANSWER ✔✔The distance between the lowest limit of
detection and a reagent blank need to be at least 2SD+2SD.
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, What is the molar absorptivity (ε) of a compound which produces an
absorbance (A) of 0.250 at a concentration of 0.200 M in a cuvette path
width of 2 cm? - 🧠 ANSWER ✔✔0.625 /(M• cm)
A chromophore has a molecular weight of 150 g/mol. When 20.0 mg of this
substance is placed in 500 mL of water, it produced an absorbance of
0.250. What is the molar absorptivity (ε) of this compound? (The cuvette
path width is 1 cm). - 🧠 ANSWER ✔✔938 /(M• cm)
The concentration of a solution is 10.0 M. It has an absorbance of 0.200
when placed in a 1.00 cm cuvette at the wavelength of 250 nm. What
would be the absorbance if the solution is 5.00 M? Assume both
concentrations are in the analytical range (within linear) - 🧠 ANSWER
✔✔0.100
A compound has a molar absorptivity of 1.50 x 102 /(M• cm) . if 1.0 mL of a
solution containing this compound is placed in 5.0 mL of water, this final
solution produced and absorbance of 1.500. What is the concentration of
this compound in the original solution? (cuvette = 1.0 cm) - 🧠 ANSWER
✔✔0.060 M
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