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BLD 313 Exam 3 – 2025/2026 Clinical Lab Science Study Guide with Verified Q&A on Spectrophotometry, Enzymes, and Immunoassays

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This comprehensive exam prep guide for BLD 313 includes 100% verified answers to questions from the 2025/2026 third exam, covering core clinical laboratory science concepts. Topics include spectrophotometry, molar absorptivity, enzyme kinetics, fluorescence and chemiluminescence, nephelometry, immunoassay formats (ELISA, EMIT, CEDIA), and electrophoresis. Designed in a clear Q&A format, this document is ideal for practical revision and deep understanding of advanced lab techniques.

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BLD 313 Exam 3 2025/2026 Exam
Questions with 100% Correct Answers |
Latest Update



Which of the following is an example of semi-quantitative test result? - 🧠

ANSWER ✔✔3+


Which of the following signals is light based? - 🧠 ANSWER ✔✔scatter


Which of the following under-estimates the concentration but not over-

estimates? - 🧠 ANSWER ✔✔downward shift


What is the purpose of the reagent blank in spectrophotometric analysis? -

🧠 ANSWER ✔✔to subtract the color contribution of the reagent


Which of the following describes a specimen blank? - 🧠 ANSWER

✔✔subtract the light absorption of patient serum.


Which of the following statement is true about lower limit of detection and

reagent blank? - 🧠 ANSWER ✔✔The distance between the lowest limit of

detection and a reagent blank need to be at least 2SD+2SD.


COPYRIGHT©NINJANERD 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER:
619652435. TERMS OF USE. PRIVACY STATEMENT. ALL RIGHTS RESERVED 1

, What is the molar absorptivity (ε) of a compound which produces an

absorbance (A) of 0.250 at a concentration of 0.200 M in a cuvette path

width of 2 cm? - 🧠 ANSWER ✔✔0.625 /(M• cm)


A chromophore has a molecular weight of 150 g/mol. When 20.0 mg of this

substance is placed in 500 mL of water, it produced an absorbance of

0.250. What is the molar absorptivity (ε) of this compound? (The cuvette

path width is 1 cm). - 🧠 ANSWER ✔✔938 /(M• cm)


The concentration of a solution is 10.0 M. It has an absorbance of 0.200

when placed in a 1.00 cm cuvette at the wavelength of 250 nm. What

would be the absorbance if the solution is 5.00 M? Assume both

concentrations are in the analytical range (within linear) - 🧠 ANSWER

✔✔0.100


A compound has a molar absorptivity of 1.50 x 102 /(M• cm) . if 1.0 mL of a

solution containing this compound is placed in 5.0 mL of water, this final

solution produced and absorbance of 1.500. What is the concentration of

this compound in the original solution? (cuvette = 1.0 cm) - 🧠 ANSWER

✔✔0.060 M




COPYRIGHT©NINJANERD 2025/2026. YEAR PUBLISHED 2025. COMPANY REGISTRATION NUMBER:
619652435. TERMS OF USE. PRIVACY STATEMENT. ALL RIGHTS RESERVED 2

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