MICR 4423 ANTIBIOTIC AND ANTIBIOTIC
RESISTANCES EXAM 3
what is the target of abyssomycin? - Answers :Folate synthesis/metabolism
what is the target of aminoglycosides? - Answers :protein synthesis
what is the target of chloramphenicol? - Answers :protein synthesis
what is the target of dalfopristin? - Answers :protein synthesis
what is the target of fluoroquinolones? - Answers :DNA gyrase / Topo 4
what is the target of kasugamycin? - Answers :protein synthesis
what is the target of kirromycin? - Answers :protein synthesis
what is the target of LFF571? - Answers :protein synthesis
what is the target of lincomycin? - Answers :protein synthesis
what is the target of lipiarmycin? - Answers :transcription
what is the target of macrolides? - Answers :protein synthesis
what is the target of mupirocin? - Answers :protein synthesis
what is the target of quinupristin? - Answers :protein synthesis
what is the target of rifamycins? - Answers :transcription
what is the target of sulfa drugs? - Answers :folate synthesis/metabolism
what is the target of oxasolidinones? - Answers :protein synthesis
what is the target of tetracyclines? - Answers :protein synthesis
what is the target of trimethoprim? - Answers :folate synthesis/metabolism
which two antibiotics interfere with the A site on 30S subunit? - Answers :tetracyclines
aminoglycosides
what antibiotic interferes with th Ile-tRNA synthetase? - Answers :mypirocin
which two antibiotics interfere with EF-Tu? - Answers :kirromycin
, LFF571
Which antibiotics interfere with he exit tunnel in 50s subunit? - Answers :macrolides
lincomycin
quinupristin
what antibiotic interferes with the P site on 30S subunit? - Answers :kasugamycin
which antibiotics interfere with the PTC on 50S subunit? - Answers :dalfopristin
chloramphenicol
oxazolidinones
what components are needed for bacterial translation? - Answers :mRNA
charged tRNA (A.A.)
ribosomal subunits (rRNA + proteins)
Initiation factors
EF-Tu
EF-G
How does kasugamycin act as an antibiotic? - Answers :Targets initiation. Competes
with initiator tRNA at the P & E sites of the 30S subunit and blocks translation initiation.
It disrupts interaction between initiator tRNA and start codon.
*Makes the mRNA "naked" (no ribosomes)
what is unique about the structure of tetracyclines? - Answers :four ring structure
examples of tetracyclines: - Answers :"plain" tetracycline
Terramycin (oxytetracycline)
Aureomycin (clortetracycline)
doxycycline
tigecycline
eravacycline
How do tetracyclines act as antibiotics? - Answers :*Binds to the 30S subunit at the A
site
*Interacts with the 16S rRNA and codon at the A site by overlapping with them.
*Destabilizes codon-anticodon interactions at the A site
*blocks elongation (accommodation)
How do aminoglycosides act as antibiotics? - Answers :*Binds 30S subunit (16srRNA)
at the A site
*Blocks elongation (accommodation)
*Stabilizes mismatches at the A site
**Causes misfolded proteins -> rapidly bactericidal
***Increases misincorporation
RESISTANCES EXAM 3
what is the target of abyssomycin? - Answers :Folate synthesis/metabolism
what is the target of aminoglycosides? - Answers :protein synthesis
what is the target of chloramphenicol? - Answers :protein synthesis
what is the target of dalfopristin? - Answers :protein synthesis
what is the target of fluoroquinolones? - Answers :DNA gyrase / Topo 4
what is the target of kasugamycin? - Answers :protein synthesis
what is the target of kirromycin? - Answers :protein synthesis
what is the target of LFF571? - Answers :protein synthesis
what is the target of lincomycin? - Answers :protein synthesis
what is the target of lipiarmycin? - Answers :transcription
what is the target of macrolides? - Answers :protein synthesis
what is the target of mupirocin? - Answers :protein synthesis
what is the target of quinupristin? - Answers :protein synthesis
what is the target of rifamycins? - Answers :transcription
what is the target of sulfa drugs? - Answers :folate synthesis/metabolism
what is the target of oxasolidinones? - Answers :protein synthesis
what is the target of tetracyclines? - Answers :protein synthesis
what is the target of trimethoprim? - Answers :folate synthesis/metabolism
which two antibiotics interfere with the A site on 30S subunit? - Answers :tetracyclines
aminoglycosides
what antibiotic interferes with th Ile-tRNA synthetase? - Answers :mypirocin
which two antibiotics interfere with EF-Tu? - Answers :kirromycin
, LFF571
Which antibiotics interfere with he exit tunnel in 50s subunit? - Answers :macrolides
lincomycin
quinupristin
what antibiotic interferes with the P site on 30S subunit? - Answers :kasugamycin
which antibiotics interfere with the PTC on 50S subunit? - Answers :dalfopristin
chloramphenicol
oxazolidinones
what components are needed for bacterial translation? - Answers :mRNA
charged tRNA (A.A.)
ribosomal subunits (rRNA + proteins)
Initiation factors
EF-Tu
EF-G
How does kasugamycin act as an antibiotic? - Answers :Targets initiation. Competes
with initiator tRNA at the P & E sites of the 30S subunit and blocks translation initiation.
It disrupts interaction between initiator tRNA and start codon.
*Makes the mRNA "naked" (no ribosomes)
what is unique about the structure of tetracyclines? - Answers :four ring structure
examples of tetracyclines: - Answers :"plain" tetracycline
Terramycin (oxytetracycline)
Aureomycin (clortetracycline)
doxycycline
tigecycline
eravacycline
How do tetracyclines act as antibiotics? - Answers :*Binds to the 30S subunit at the A
site
*Interacts with the 16S rRNA and codon at the A site by overlapping with them.
*Destabilizes codon-anticodon interactions at the A site
*blocks elongation (accommodation)
How do aminoglycosides act as antibiotics? - Answers :*Binds 30S subunit (16srRNA)
at the A site
*Blocks elongation (accommodation)
*Stabilizes mismatches at the A site
**Causes misfolded proteins -> rapidly bactericidal
***Increases misincorporation
