Instrumental Analysis: Chromatography questions with
verified answers
1 theoretical plate Ans✓✓✓ as analyte moves through a column
transitions from the mobile phase to stationary phase then back to the
mobile phase
2 most common detection methods Ans✓✓✓ Silver stain and
comassive blue
Actual pH meter design Ans✓✓✓ |reflect|[H₃O⁺]=a|glass membrane|
H₃O=a₂ ref electrode
-The pH measurment depends on the boundry potential that develops
due to difference on [H⁺] inside and [H⁺] outside
Adsorption Ans✓✓✓ Stationary phase calcium carbonate, sugar silica,
alumina, mobile phase-water, organic solvents
Atomic Emission Detectors Ans✓✓✓ eleunt passed into a helium
plasma. moniter for specific elements-compared w/ other methods
Basic Technique Ans✓✓✓ Two phases: Mobile phase and stationary
phase. Place mixture onto the stationary phase and moves throught
stationary phase with the mobile phase
, Before running gel Ans✓✓✓ add sodium dodecyl sulfate(SDS)--
hydrophobic region(organic) and hydrophilic region(inorganic)
capillary column Ans✓✓✓ stationary phase-highly purified fused
silicon-internal diameter 0.1-0.35 mm length: 15-100 m. outside coated
with poly imide or aluminum stationary phase-coats inside of the tube.
wallcoated open tubular, film thickness=0.05-0.5 micrometer, can get
150,000 theoretical plates
Cation Exchange Ans✓✓✓ R-SO3⁻ + H⁺ ↔ R-SO3⁻ Na⁺ + H⁺
Stationary + mobile ↔ Stationary + mobile
Cell Potential and Reactant Concentration Ans✓✓✓ ΔG=ΔG⁰+RTln(Q)
where ΔG=-nFE and ΔG⁰=-NFE⁰ F=96500 C/mol
cold on-column injection Ans✓✓✓ sample placed on column cold then
vaporized
Column or plate Ans✓✓✓ stationary attached or packed into column
Commasie blue Ans✓✓✓ binds to amine groups in proteins.
Commasie blue Ans✓✓✓ sens limit (~0.02 mg) -not mass spec
compatible
verified answers
1 theoretical plate Ans✓✓✓ as analyte moves through a column
transitions from the mobile phase to stationary phase then back to the
mobile phase
2 most common detection methods Ans✓✓✓ Silver stain and
comassive blue
Actual pH meter design Ans✓✓✓ |reflect|[H₃O⁺]=a|glass membrane|
H₃O=a₂ ref electrode
-The pH measurment depends on the boundry potential that develops
due to difference on [H⁺] inside and [H⁺] outside
Adsorption Ans✓✓✓ Stationary phase calcium carbonate, sugar silica,
alumina, mobile phase-water, organic solvents
Atomic Emission Detectors Ans✓✓✓ eleunt passed into a helium
plasma. moniter for specific elements-compared w/ other methods
Basic Technique Ans✓✓✓ Two phases: Mobile phase and stationary
phase. Place mixture onto the stationary phase and moves throught
stationary phase with the mobile phase
, Before running gel Ans✓✓✓ add sodium dodecyl sulfate(SDS)--
hydrophobic region(organic) and hydrophilic region(inorganic)
capillary column Ans✓✓✓ stationary phase-highly purified fused
silicon-internal diameter 0.1-0.35 mm length: 15-100 m. outside coated
with poly imide or aluminum stationary phase-coats inside of the tube.
wallcoated open tubular, film thickness=0.05-0.5 micrometer, can get
150,000 theoretical plates
Cation Exchange Ans✓✓✓ R-SO3⁻ + H⁺ ↔ R-SO3⁻ Na⁺ + H⁺
Stationary + mobile ↔ Stationary + mobile
Cell Potential and Reactant Concentration Ans✓✓✓ ΔG=ΔG⁰+RTln(Q)
where ΔG=-nFE and ΔG⁰=-NFE⁰ F=96500 C/mol
cold on-column injection Ans✓✓✓ sample placed on column cold then
vaporized
Column or plate Ans✓✓✓ stationary attached or packed into column
Commasie blue Ans✓✓✓ binds to amine groups in proteins.
Commasie blue Ans✓✓✓ sens limit (~0.02 mg) -not mass spec
compatible
