Ascp Scym Study Questions With Correct Answers

ASCP SCYM STUDY QUESTIONS WITH
CORRECT ANSWERS



Sample bProcessing b- bcorrect banswer✔✔Optimized bantibody bconcentration
Defined brange bof bcell b#
Define bsample bhandling, btime band btemperature
Define bacceptable btime bof bdelay bfor bacquisition

CD2 b- bcorrect banswer✔✔T bcells: bNK bcells

CD3 b- bcorrect banswer✔✔T bcells

CD4 b- bcorrect banswer✔✔T bhelper bcells, bmonocytes b(dimly bexpressed)

CD5 b- bcorrect banswer✔✔T bcells; bSome bB bCells

CD8 b- bcorrect banswer✔✔T bcytotoxic bcells, bmacrophages

CD10 b- bcorrect banswer✔✔Immature bT, bB bCells, bPMN's

CD13 b- bcorrect banswer✔✔Monocytes, bPMN's

CD14 b- bcorrect banswer✔✔Monocytes, bPMN's b(dimly bexpressed)

CD16 b- bcorrect banswer✔✔NK bcells, bPMN's, bmonocytes

CD19 b- bcorrect banswer✔✔B bCells

CD20 b- bcorrect banswer✔✔B bCells

CD25 b- bcorrect banswer✔✔Activated bT bCells

CD33 b- bcorrect banswer✔✔Monocytes

CD34 b- bcorrect banswer✔✔Stem/Progenitor bCells

,CD45 b- bcorrect banswer✔✔Leukocytes

CD56 b- bcorrect banswer✔✔NK bcells, bmonocytes b(dimly bexpressed)

Anti-Kappa b- bcorrect banswer✔✔B bCells

Anti-Lambda b- bcorrect banswer✔✔B bCells

Tdt b- bcorrect banswer✔✔Immature bLymphocytes

Monoclonal bAntibody bStaining bDirect bStaining b- bcorrect banswer✔✔Rapid bsingle
bstep bstaining
Can buse bmultiple bantibodies bfrom bthe bsame bhost

Monoclonal bAntibody bStaining bIndirect bStaining b- bcorrect banswer✔✔Secondary
bamplifies bsingle
A bfew blabeled bsecondaries bcan bdetect bmany bprimaries

Clinical bApplications bof bFlow b- bcorrect banswer✔✔-Instrument bPerformance
-Integrity bof bthe bSpecimen
-Quality bin bProcessing bof bSpecimens
-Appropriate bReagents band bControls
-Qualified bTechnical bStaff
-Regulatory brequirements bfrom bCAP, bTJC, bCLIA, band bthe bFDA bexist bto bensure
bthe baccurate breporting bof bflow bcytometric bresults.


Lymphocytes bSubset bEnumeration b(Immunophenotyping) b- bcorrect banswer✔✔2
bTubes: b1- bCD3/CD4/CD8/CD45 b
b2- bCD3/CD19/CD16&CD56/CD45
-May binclude banalysis bof bCD4 band bCD8 bT bCells, bB bCells band bNK bCells.
-Results breported bas bproportion b(%) bof btotal blymphocyte bpopulation.

CD4 bCounts bin bHIV bInfection b- bcorrect banswer✔✔-Asses bdegree bof
bimmunodeficiency
-Diagnose bof bAIDS
-Institution bof bAnti-Retroviral b(ARV) btherapy band bopportunistic binfection b(OI)
bprophylaxis
-Monitoring bimmune breconstitution

CD4 bImmunophenotyping bPanels b- bcorrect banswer✔✔

2 bcolor b- bcorrect banswer✔✔Isotype bCtrl
CD45/CD14
CD3/CD4
CD3/CD8

, CD3/CD19
CD3/CD16 b+ b56

3 bColor b- bcorrect banswer✔✔CD3/CD4/CD45
CD3/CD8/CD45
CD3/CD19/CD16 b+ b56

4 bColor b- bcorrect banswer✔✔CD3/CD4/CD8/CD45
CD3/CD16/CD16 b+ b56/CD45

Determination bof bAbsolute bCounts b- bcorrect banswer✔✔-Subset b% bX bAbsolute
bLymphocyte bCount
-Counting bBeads- bknown bconcentration bpresent bin bstaining btube bor badded bto btube
-Volumetric bCytometers

Leukemia/Lymphoma bPhenotyping b- bcorrect banswer✔✔-Important bin bDiagnosis,
bClassification band bMonitoring bof bpatients bwith bhematologic bmalignancies.
-Utility bbased bon bthe bfact bthat bhematopoiesis bis bcharacterized bby bthe bgain band
bloss bof bcell bsurface band bintracellular bantigen bduring bthe bprocess bof bmaturation.
-Malignant bcells bmay bhave ba bblock bat ban bimmature bstage bresulting bin babnormal
bnumbers bof bimmune bcells. bWith ba bmature bphenotype bmay bbe bpresent bin
babnormally bhigh bnumbers. bMay bhave ban baberrant bphenotype.


Leukemia/Lymphoma bPhenotyping bFC b- bcorrect banswer✔✔-Analysis bwith bpanels bof
bmonoclonal bantibodies ballows bfor blineage bassignment band bsub bclassification bthat
bmay bhave bfurther bprognostic bsignificance.
-The bidentification bof ba bunique bphenotype bcan balso bfacilitate bpost-therapy
bmonitoring bto bassess bresidual bdisease.


Relevance bfor bthe bDiagnosis band bClassification bof bHematologic bMalignancies b-
bcorrect banswer✔✔-Expression bof baberrant blineage bassociated bantigen
-Absence bof blineage bassociated bantigen
-Asynchronous bexpression bof bantigens
-Abnormal blevel bof bexpression bof bantigen

Detection bof bFetal bHemoglobin b- bcorrect banswer✔✔-Detection bof bfetal bhemoglobin
bcontaining bcells bin buseful bfor bthe bdiagnosis band bmanagement bof bfeto-maternal
bhemorrhage
-Traditionally bassayed bwith bthe bKleihauer-Betke bmethod b
-FC boffers bimproved bsensitivity b(<0.02% bfetal bRBCs) band breproducibility b(CV b<15%
b
-Method: bIntracellular bstaining bwith ba bfetal bhemoglobin bspecific bmonoclonal
bantibody


CD34 bStem bCell bEnumeration b- bcorrect banswer✔✔-Present bon bimmature
bhematopoietic bcells
-Expression bis blost bas bcells bmature