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DAT lab study Questions and Complete Solutions Graded A+

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DAT lab study Questions and Complete Solutions Graded A+

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DAT lab study Questions and
Complete Solutions Graded A+
what is the purpose of performing the DAT procedure? - Answer: used to determine if red cells have
been coated in vivo with immunoglobulin, complement or both.



what does a positive DATE indicate? - Answer: indicates that red cells are coated with protein in-vivo



what 3 reagents may be used in the DAT procedure? - Answer: polyspecific IgG, C3d



monospecific IgG or C3d



what is the sample of choice for the DAT? Why? - Answer: whole blood collected in an EDTA tube
because it prevents in-vitro fixation of complement.



the DAT is used to assist in hemolysis associated with what 4 clinical conditions: - Answer: HDFN

autoimmune hemolytic anemia

red cell sensitization

transfusion rxns



what 3 items in the pt history are crucial in determining the clinical significance of a positive DAT? -
Answer: pts diagnosis

transfusion and pregnancy hx

recent/current medications



state the principle of the DAT procedure - Answer: the detection of abs that attached to red cells in-vivo



what ab class is involved in HDFN? - Answer: IgG

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