MOCK CG(ASCP) LATEST
CERTIFICATION EXAM 1
- PART 1
ANSWER ALL QUESTIONS IN THIS SECTION
QUESTION 1
What factors severs to increase stringency of post-hybridization washes?
a. Increase water concentration
b. Increase salt concentration
c. Decrease temperature
d. None of the above - ANSWERS-Increase water concentration
QUESTION 2
The field diaphragm on the microscope is used to:
a. Change the angle of light inside the objective
b. Adjust brightness
c. Adjust resolution and contrast
d. View the light beam for centering and control of glare - ANSWERS-View the
light beam for centering and control of glare
QUESTION 3
END OF
PAGE
1
, MOCK CG(ASCP) LATEST
CERTIFICATION EXAM 1
- PART 1
Before photographing a cell, you must perform the following steps of Koehler
illumination:
1. Open the field diaphragm until the entire field is illuminated and the diaphragm
leaves are just outside the field of view.
2. Focus on the field diaphragm leaves using the condenser focus knob.
3. Focus on the specimen.
4. Close the field diaphragm all the way.
Which of the following describes the proper order of these steps?
a. 4,3,1,2
b. 2,1,3,4
c. 1,2,3,4
d. 3,4,2,1 - ANSWERS-3,4,2,1
QUESTION 4
END OF
PAGE
2
, MOCK CG(ASCP) LATEST
CERTIFICATION EXAM 1
- PART 1
Which of the following factors do not, or cannot be used to affect resolution on a
microscope?
a. The numerical aperture of the objective and condenser
b. The refractive index of the immersion oil
c. The field stop diaphragm located at the base of the microscope
d. The aperture diaphragm located within the condenser - ANSWERS-The field
stop diaphragm located at the base of the microscop
QUESTION 5
At the beginning of the workday, a technologist could not see a clear image
through the 100X objective although a clear image was obtained when observing
the slide with the 10X objective. What is the probable cause?
a. Too much oil is on the slide
b. The light source is not centered
c. The lens is in the locked position
d. Two coverslips are on the slide - ANSWERS-Two coverslips are on the slide
QUESTION 6
END OF
PAGE
3
, MOCK CG(ASCP) LATEST
CERTIFICATION EXAM 1
- PART 1
When processing an image on an automated karyotyping system, it is necessary to
convert the analog image seen through the microscope to a digital image. This
process is called:
a. Segmentation
b. Digitization
c. Thresholding
d. Pixelation - ANSWERS-Digitization
QUESTION 7
When capturing live images, what feature is adjusted first in order to ensure that
the entire chromosome is visualized (including the satellites of acrocentric
chromosomes)?
a. Threshold
b. Bit map
c. Pixel
d. CCD camera - ANSWERS-Threshold
Parfocal refers to:
END OF
PAGE
4
CERTIFICATION EXAM 1
- PART 1
ANSWER ALL QUESTIONS IN THIS SECTION
QUESTION 1
What factors severs to increase stringency of post-hybridization washes?
a. Increase water concentration
b. Increase salt concentration
c. Decrease temperature
d. None of the above - ANSWERS-Increase water concentration
QUESTION 2
The field diaphragm on the microscope is used to:
a. Change the angle of light inside the objective
b. Adjust brightness
c. Adjust resolution and contrast
d. View the light beam for centering and control of glare - ANSWERS-View the
light beam for centering and control of glare
QUESTION 3
END OF
PAGE
1
, MOCK CG(ASCP) LATEST
CERTIFICATION EXAM 1
- PART 1
Before photographing a cell, you must perform the following steps of Koehler
illumination:
1. Open the field diaphragm until the entire field is illuminated and the diaphragm
leaves are just outside the field of view.
2. Focus on the field diaphragm leaves using the condenser focus knob.
3. Focus on the specimen.
4. Close the field diaphragm all the way.
Which of the following describes the proper order of these steps?
a. 4,3,1,2
b. 2,1,3,4
c. 1,2,3,4
d. 3,4,2,1 - ANSWERS-3,4,2,1
QUESTION 4
END OF
PAGE
2
, MOCK CG(ASCP) LATEST
CERTIFICATION EXAM 1
- PART 1
Which of the following factors do not, or cannot be used to affect resolution on a
microscope?
a. The numerical aperture of the objective and condenser
b. The refractive index of the immersion oil
c. The field stop diaphragm located at the base of the microscope
d. The aperture diaphragm located within the condenser - ANSWERS-The field
stop diaphragm located at the base of the microscop
QUESTION 5
At the beginning of the workday, a technologist could not see a clear image
through the 100X objective although a clear image was obtained when observing
the slide with the 10X objective. What is the probable cause?
a. Too much oil is on the slide
b. The light source is not centered
c. The lens is in the locked position
d. Two coverslips are on the slide - ANSWERS-Two coverslips are on the slide
QUESTION 6
END OF
PAGE
3
, MOCK CG(ASCP) LATEST
CERTIFICATION EXAM 1
- PART 1
When processing an image on an automated karyotyping system, it is necessary to
convert the analog image seen through the microscope to a digital image. This
process is called:
a. Segmentation
b. Digitization
c. Thresholding
d. Pixelation - ANSWERS-Digitization
QUESTION 7
When capturing live images, what feature is adjusted first in order to ensure that
the entire chromosome is visualized (including the satellites of acrocentric
chromosomes)?
a. Threshold
b. Bit map
c. Pixel
d. CCD camera - ANSWERS-Threshold
Parfocal refers to:
END OF
PAGE
4
