MIC 205A Final Review Exam 2025
Questions and Answers
Hydramacin is a protein that is 60 amino acids long. How many nucleobases would the mRNA
encoding hydramacin have - ANS 180
Scientists discovered this novel protein while studying what mechanism in Hydra? - ANS Skin
defenses
3Hydramacin is unique, but appears to be most similar to proteins found in - ANS Scorpion
venom
the Kirby-Bauer disc diffusion test is used to determine: - ANS antimicrobial susceptibility
During incubation, antimicrobials applied to the bacterial lawn become increasingly _______ as
they diffuse out of the disc into the medium. - ANS diluted (The antimicrobial agents move
from a high concentration on the disc to a lower concentration within the medium via diffusion)
The area of clearing around the discs is called the zone of: - ANS inhibition (A zone of
inhibition is the clear area that develops around a test bacterium sensitive to the applied
antimicrobial agent.)
the diameter of the zone of inhibition is always measured in mm. - ANS The diameter of the
zone of inhibition is measured in millimeters (mm) to determine susceptibility to antimicrobial
agents.
Pg. 1 Copyright © 2025 Jasonmcconell. ALL RIGHTS RESERVED.
, Which of the medications tested in the above image is a beta-lactam antibiotic? -
ANS ampicillin(Ampicillin is a semisynthetic penicillin that targets the cell wall.)
Which of the following medications created the largest zone of inhibition -
ANS chloramphenicol (The largest zone of inhibition was measured at 27 mm around the disc
containing chloramphenicol.)
Please evaluate the test organism's sensitivity to chloramphenico - ANS sensitive(The zone of
inhibition measured 27 mm for this drug, which is classified as sensitive to this medication)
What is the test organism's level of sensitivity to tetracycline? - ANS intermediate( The zone
of inhibition measured 15 mm for this medication, which is classified as resistant to this
medication. Less than 14 mm indicates resistance to the medication, while more than 19 mm
would indicate sensitivity to the medication. Please refer to sections 20.1 and 20.4 for
additional information)
Based upon the information in the case, hydramacin can be classified as what type of
antibiotic? - ANS broad spectrum ( Hydramacin appears to be effective against both Gram-
positive and Gram-negative bacteria making it a broad spectrum antibiotic.)
If hydramacin exhibits selective toxicity in experiments, it will - ANS cause no damage to host
tissues (A medication exhibiting selective toxicity kills or inhibits microbial cells but does not
cause host cells any damage. Please refer to sections 20.1 and 20.4 for aditional information)
Hydramacin needs to be tested in human trials - ANS true (One important phase of testing is
to determine if a compound has any toxic effects on the patient.
If the Kirby-Bauer assay revealed any zone of inhibition for hydramacin, this would mean that
the test organism was sensitive to this novel drug. - ANS False (False, the measurement of
the diameter of the zone of inhibition would have to be compared to the established range for
these values in order to determine if the organism was in fact sensitive to hydramacin.
Pg. 2 Copyright © 2025 Jasonmcconell. ALL RIGHTS RESERVED.
Questions and Answers
Hydramacin is a protein that is 60 amino acids long. How many nucleobases would the mRNA
encoding hydramacin have - ANS 180
Scientists discovered this novel protein while studying what mechanism in Hydra? - ANS Skin
defenses
3Hydramacin is unique, but appears to be most similar to proteins found in - ANS Scorpion
venom
the Kirby-Bauer disc diffusion test is used to determine: - ANS antimicrobial susceptibility
During incubation, antimicrobials applied to the bacterial lawn become increasingly _______ as
they diffuse out of the disc into the medium. - ANS diluted (The antimicrobial agents move
from a high concentration on the disc to a lower concentration within the medium via diffusion)
The area of clearing around the discs is called the zone of: - ANS inhibition (A zone of
inhibition is the clear area that develops around a test bacterium sensitive to the applied
antimicrobial agent.)
the diameter of the zone of inhibition is always measured in mm. - ANS The diameter of the
zone of inhibition is measured in millimeters (mm) to determine susceptibility to antimicrobial
agents.
Pg. 1 Copyright © 2025 Jasonmcconell. ALL RIGHTS RESERVED.
, Which of the medications tested in the above image is a beta-lactam antibiotic? -
ANS ampicillin(Ampicillin is a semisynthetic penicillin that targets the cell wall.)
Which of the following medications created the largest zone of inhibition -
ANS chloramphenicol (The largest zone of inhibition was measured at 27 mm around the disc
containing chloramphenicol.)
Please evaluate the test organism's sensitivity to chloramphenico - ANS sensitive(The zone of
inhibition measured 27 mm for this drug, which is classified as sensitive to this medication)
What is the test organism's level of sensitivity to tetracycline? - ANS intermediate( The zone
of inhibition measured 15 mm for this medication, which is classified as resistant to this
medication. Less than 14 mm indicates resistance to the medication, while more than 19 mm
would indicate sensitivity to the medication. Please refer to sections 20.1 and 20.4 for
additional information)
Based upon the information in the case, hydramacin can be classified as what type of
antibiotic? - ANS broad spectrum ( Hydramacin appears to be effective against both Gram-
positive and Gram-negative bacteria making it a broad spectrum antibiotic.)
If hydramacin exhibits selective toxicity in experiments, it will - ANS cause no damage to host
tissues (A medication exhibiting selective toxicity kills or inhibits microbial cells but does not
cause host cells any damage. Please refer to sections 20.1 and 20.4 for aditional information)
Hydramacin needs to be tested in human trials - ANS true (One important phase of testing is
to determine if a compound has any toxic effects on the patient.
If the Kirby-Bauer assay revealed any zone of inhibition for hydramacin, this would mean that
the test organism was sensitive to this novel drug. - ANS False (False, the measurement of
the diameter of the zone of inhibition would have to be compared to the established range for
these values in order to determine if the organism was in fact sensitive to hydramacin.
Pg. 2 Copyright © 2025 Jasonmcconell. ALL RIGHTS RESERVED.
