BIOL 241 Exam 3 Purdue Actual study
set with Questions and correct/verified
Answers
In eukaryotic gene regulation, which of the following events is likely to be associated with an increase in
gene transcription? - ANSW-Acetylation of lysines histone tails near a transcription unit.
Regarding the helix-turn-helix motif, which of the following statements is correct? - ANSW-a. This motif
allows proteins to bind to specific regulatory DNA sequences.
3) After analyzing the sequence of a gene, you have found that it contains leucine zippers. Based on this,
which of the following statements is correct? - ANSW-a. This gene encodes a protein that forms dimers.
4) The Drosophila DSCAM locus is capable of expressing multiple isoforms. This is accomplished by: -
ANSW-a. Alternative splicing.
5) Regarding siRNA, which of the following statements is incorrect? - ANSW-a. siRNA usually binds to
target mRNA at specific sites in the 3' UTR.
6) The Luria-Delbrück fluctuation test demonstrated that: - ANSW-a. Spontaneous mutations are
random.
7) Regarding xeroderma pigmentosum (XP), which of the following statements is incorrect? - ANSW-a. XP
is caused by mutation in the components of mismatch repair pathway.
8) Which of the following events is most likely to cause translocations? - ANSW-treating cells with X rays
9) Sequencing the ends of clones from cDNA library is known as? - ANSW-a. Expressed sequence tag
sequencing.
10) Bisulfite sequencing is typically used to determine: - ANSW-a. The methylation state of DNA in
eukaryotic chromosomes.
11) Which of the following techniques can detect the difference between two homologous
chromosomes? - ANSW-a. Restriction fragment length polymorphism.
12) The ChIP (chromatin immunoprecipitation) assays will identify: - ANSW-a. The sequences of
transcription enhancer elements.
14) The dideoxynucleotides (ddNTP) differ from dNTP in: - ANSW-a. The absence of hydroxyl group at the
3' position.
16) Which of the following approaches can be used to introduce specific changes in Drosophila genome?
- ANSW-a. Injecting embryos with specific gRNA and Cas9 nuclease.
15) Which of the following statements accurately describes the advantage of using RNAi in a genetic
screen? - ANSW-a. The identification of genes affected by RNAi is instantaneous.
set with Questions and correct/verified
Answers
In eukaryotic gene regulation, which of the following events is likely to be associated with an increase in
gene transcription? - ANSW-Acetylation of lysines histone tails near a transcription unit.
Regarding the helix-turn-helix motif, which of the following statements is correct? - ANSW-a. This motif
allows proteins to bind to specific regulatory DNA sequences.
3) After analyzing the sequence of a gene, you have found that it contains leucine zippers. Based on this,
which of the following statements is correct? - ANSW-a. This gene encodes a protein that forms dimers.
4) The Drosophila DSCAM locus is capable of expressing multiple isoforms. This is accomplished by: -
ANSW-a. Alternative splicing.
5) Regarding siRNA, which of the following statements is incorrect? - ANSW-a. siRNA usually binds to
target mRNA at specific sites in the 3' UTR.
6) The Luria-Delbrück fluctuation test demonstrated that: - ANSW-a. Spontaneous mutations are
random.
7) Regarding xeroderma pigmentosum (XP), which of the following statements is incorrect? - ANSW-a. XP
is caused by mutation in the components of mismatch repair pathway.
8) Which of the following events is most likely to cause translocations? - ANSW-treating cells with X rays
9) Sequencing the ends of clones from cDNA library is known as? - ANSW-a. Expressed sequence tag
sequencing.
10) Bisulfite sequencing is typically used to determine: - ANSW-a. The methylation state of DNA in
eukaryotic chromosomes.
11) Which of the following techniques can detect the difference between two homologous
chromosomes? - ANSW-a. Restriction fragment length polymorphism.
12) The ChIP (chromatin immunoprecipitation) assays will identify: - ANSW-a. The sequences of
transcription enhancer elements.
14) The dideoxynucleotides (ddNTP) differ from dNTP in: - ANSW-a. The absence of hydroxyl group at the
3' position.
16) Which of the following approaches can be used to introduce specific changes in Drosophila genome?
- ANSW-a. Injecting embryos with specific gRNA and Cas9 nuclease.
15) Which of the following statements accurately describes the advantage of using RNAi in a genetic
screen? - ANSW-a. The identification of genes affected by RNAi is instantaneous.
