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PRINCIPLES OF BIO II LAB FINAL EXAM QUESTIONS AND DETAILED ANSWERS.

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Which of the following best describes the mechanism of action of the antibiotic streptomycin? Multiple Choice a. Streptomycin inhibits cell wall synthesis in all bacterial species. b. Streptomycin creates mutations in bacteria which cause them to become resistant. c. It disrupts protein synthesis in resistant bacteria. d. It disrupts protein synthesis in nonresistant bacteria. - Answer d. It disrupts protein synthesis in nonresistant bacteria. Which of the following apply to the development of streptomycin resistance in E. coli? Check All That Apply a. The E. coli population has genetic variation. b. The selective agent is streptomycin. c. The mutation resulting in streptomycin resistance is random. d. E. coli changes its gene structure to become antobiotic resistant in the presence of streptomycin. - Answer a. The E. coli population has genetic variation. b. The selective agent is streptomycin. c. The mutation resulting in streptomycin resistance is random. Two agar plates, one containing the antibiotic streptomycin and one without antibiotics, are inoculated with E. coli. After incubation, the streptomycin negative plate has many bacterial colonies, while the streptomycin positive plate has only a few colonies. Samples of the colonies from the positive plate are placed on another positive plate, and after incubation many colonies grow. In this experiment, what is the selective agent? Multiple Choice a. the agar medium b. the antibiotic streptomycin c. the period of incubation

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PRINCIPLES OF BIO II LAB FINAL EXAM
QUESTIONS AND DETAILED ANSWERS.
Which of the following best describes the mechanism of action of the antibiotic streptomycin?



Multiple Choice



a. Streptomycin inhibits cell wall synthesis in all bacterial species.

b. Streptomycin creates mutations in bacteria which cause them to become resistant.

c. It disrupts protein synthesis in resistant bacteria.

d. It disrupts protein synthesis in nonresistant bacteria. - Answer d. It disrupts protein
synthesis in nonresistant bacteria.



Which of the following apply to the development of streptomycin resistance in E. coli?



Check All That Apply



a. The E. coli population has genetic variation.

b. The selective agent is streptomycin.

c. The mutation resulting in streptomycin resistance is random.

d. E. coli changes its gene structure to become antobiotic resistant in the presence of
streptomycin. - Answer a. The E. coli population has genetic variation.

b. The selective agent is streptomycin.

c. The mutation resulting in streptomycin resistance is random.



Two agar plates, one containing the antibiotic streptomycin and one without antibiotics, are
inoculated with E. coli. After incubation, the streptomycin negative plate has many bacterial
colonies, while the streptomycin positive plate has only a few colonies. Samples of the colonies
from the positive plate are placed on another positive plate, and after incubation many colonies
grow. In this experiment, what is the selective agent?



Multiple Choice

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