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Samenvatting Schema hoofdstuk 7

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Schema van hoofdstuk 7 op basis van de slides practica en lessen

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May 11, 2025
Number of pages
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2023/2024
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H7: OXIDASE POSITIEVE FERMENTEERDERS
Algemene kenmerken: Oxydase + Gram neg. Staafjes vergisten glucose reduceren nitraat tot nitriet

Commensaal Specifieke kenmerken Pathogenese / transmissie Pathogeen vermogen / pathologie Pathogeen Groei op KIA- Biochemische
of geïsoleerd uit isolatiebodems resultaat kenmerken
pathogeen welk staal?
Vibrio spp.
Pathogeen Kommavormig gebogen Reservoir: water en mens Vibrio cholerae: enteritis V. cholerae: TCBS: A K H2S- /
staafje (‘vibrio’) Infectie via: faeces Geel: sucrose +
- Besmet (zee)water Andere species: enteritis, wondinfecties, longinfecties, sepsis (Virbio, Proteus) Lactose –
Polaire flagel: beweeglijk - Eten van besmette V. vulnificus: uit Groen: sucrose – Glucose +
(rauwe) vissen, schaal- 200 sero-types (O-antigen) wondvocht (coliformen)
Katalase +, Oxydase + en schelpdieren Serotype O:1:
- Groenten gespoeld - Productie enterotoxine  cholera Mc Conkey:
Aëroob of facultatief met besmet water - Abrupt braken en levensbedreigende waterige kleurloos
anaëroob Faeces  water  voeding diarree: 15-20 liters/dag
- Uiteindelijk rijstwaterdiarree: kleurloos, geurloos,
Vergisten glucose zonder Vnl. import zonder eiwitten, met mucus
gas Endemisch: Zuid-Azië - Cholera toxine: verlies van vocht en elektrolyten:
Na+, K+, HCO3  hypokaliëmie (gevaar voor
Makkelijk in cultuur te Infectieuze dosis: > 108 CFU hartritme en nierblokkage)
brengen Verminder maagzuur: 103-105 - Niet behandeld: sterft door hypovolemische shock
CFU en metabole acidose
- O:1 Biotype El Tor : dominante epidemische type
- Opsporen: agglutinatietest
NAG-Virbio’s:
- Niet agglutinerende vibrio’s
- Produceren geen toxine, behalve serotype O:139 en
O:37 (ook enteritis)

Volgende: te vinden in (sub)tropisch zeewater + halofiel
V. parahaemolyticus:
- Enteritis (rauwe zeevruchten)
- Wondinfecties
V. alginolyticus en V. vulnificus:
- Wondinfecties
- Otitis

TCBS-agar bodem (thiosulfaat-citraat-bile-sucrose):

- In deze bodem zitten galzouten waartegen vibrio’s bestand zijn, sucrose, zout en een indicator  als de sucrose omgezet word naar een zuur, dan zal de indicator
verkleuren, naar geel en geeft gele kolonies
- Meer info: cursus p. 99
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