MLS Chemistry Exam || solved correctly.
Which of the following lamps provides a continuous spectrum of radiant energy in the visible,
near IR and near UV regions of the spectrum? correct answers Tungsten filament
Which of the following isolates light within a narrow region of the spectrum? correct answers
Monochromator
Which of the following is not descriptive of a photomultiplier tube? correct answers Cannot be
used with a chopper
Which of the following is false about a photomultiplier tube? correct answers Is composed of an
iron plate and a layer of selenium
Which type of photodetector employs a linear arrangement that allows it to respond to a specific
wavelength resulting in complete UV visible spectrum analysis? correct answers Photodiode
array
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass
filter used to assess? correct answers Wavelength accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank? correct answers Correct
for color contribution of the reagents
In regard to bichromatic analysis, which of the following is false? correct answers Absorbance is
measured at the spectral absorbance peak for a blank and the sample using the same wavelength
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the
wavelengths that are permitted to impinge on the sample will be within what wavelength range?
correct answers 535 to 545 nm
,Which of the following formulas is an expression of the Beer Lambert law that is routinely
applied to spectrophotometric analysis? correct answers Au X Cs / As = Cu
In spectrophotometry, which of the following is a mathematical expression of the relationship
between absorbance and transmittance? correct answers A = 2 - log % T
Which of the following is not a problem inherent in turbidimetry? correct answers Need to
maintain a constant and specific temperature
Which of the following may be associated with reflectance spectrophotometry as it relates to the
dry reagent slide technique? correct answers Unabsorbed, reflected light detected by
photodetector
Fluorometers are designed so that the path of the exciting light is at a right angle to the path of
the emitted light. What is the purpose of this design? correct answers Prevent excitation light
from reaching the detector
Which of the following represents a primary advantage of performing fluorometric over
absorption spectroscopic methods of analysis? correct answers Increased specificity and
increased sensitivity
Which of the following may be associated with fluorescence polarization? correct answers Plane
polarized light is used for sample excitation
Which of the following may be associated with bioluminescence? correct answers Light
emission produced due to enzymatic oxidation of a substrate
Nephelometry is based on the measurement of light that is: correct answers Scattered by particles
in suspension
,Which of the following instruments is used in the clinical lab or in reference labs to detect beta
and gamma emissions? correct answers Scintillation counter
Which of the following best describes chemiluminescence? correct answers Chemical energy
excites electrons that emit light upon return to ground state
In assaying an analyte with a single beam atomic absorption spectrophotometer, what is the
instrument actually measuring? correct answers Intensity of the beam from the hollow cathode
lamp after it has passed through the analyte containing flame
What is the function of the flame in atomic absorption spectroscopy? correct answers Bring the
metal analyte to its ground state
Most atomic absorption spectrophotometers incorporate a beam chopper and a tuned amplifier.
The purpose of these components is to avoid errors that would be caused by: correct answers
Measurement of light emitted by the analyte
In potentiometry, which of the following is considered the standard electrode? correct answers
Hydrogen electrode
In an electrolytic cell, which of the following is the half cell where reduction takes place? correct
answers Cathode
Mercury covered by a layer of mercurous chloride in contact with saturated potassium chloride
solution is a description of which of the following types of electrodes? correct answers Calomel
When a pH sensitive glass electrode is not actively in use, in what type of solution should it be
kept? correct answers The medium recommended by the manufacturer
, When measuring K positive with an ion selective electrode by means of a liquid ion exchange
membrane, what antibiotic will be incorporated into the membrane? correct answers
Valinomycin
Which of the following is false about ion selective electrode analysis of sodium? correct answers
Membrane coated with valinomycin
What are the principles of operation for a chloride analyzer that generates silver ions as part of
its reaction mechanism? correct answers Amperometry and coulometry
When quantifying glucose using an amperometric glucose electrode system, which of the
following is not a component of the system? correct answers Hexokinase reacts with glucose
To calibrate the pH electrode in a pH blood gas analyzer, it is necessary that: correct answers
Two buffer solutions of known pH be used
The measurement of CO2 in blood by means of a PCO2 electrode is dependent on the: correct
answers Change in pH because of increased carbonic acid in the electrolyte surrounding the
electrodes
The measurement of oxygen in blood by means of a PO2 electrode involves which of the
following? correct answers Direct relationship between amount of oxygen in the sample and
amount of current flowing in the measuring system
Which of the following blood gas parameters are measured directly by the blood gas analyzer
electrochemically as opposed to being calculated by the instrument? correct answers pH, PCO2,
PO2
Which of the following statements is false about anodic stripping voltammetry (ASV)? correct
answers Based on potentiometry
Which of the following lamps provides a continuous spectrum of radiant energy in the visible,
near IR and near UV regions of the spectrum? correct answers Tungsten filament
Which of the following isolates light within a narrow region of the spectrum? correct answers
Monochromator
Which of the following is not descriptive of a photomultiplier tube? correct answers Cannot be
used with a chopper
Which of the following is false about a photomultiplier tube? correct answers Is composed of an
iron plate and a layer of selenium
Which type of photodetector employs a linear arrangement that allows it to respond to a specific
wavelength resulting in complete UV visible spectrum analysis? correct answers Photodiode
array
When performing spectrophotometer quality assurance checks, what is the holmium oxide glass
filter used to assess? correct answers Wavelength accuracy
In spectrophotometric analysis, what is the purpose of the reagent blank? correct answers Correct
for color contribution of the reagents
In regard to bichromatic analysis, which of the following is false? correct answers Absorbance is
measured at the spectral absorbance peak for a blank and the sample using the same wavelength
The bandpass of a spectrophotometer is 10 nm. If an instrument is set at 540 nm, the
wavelengths that are permitted to impinge on the sample will be within what wavelength range?
correct answers 535 to 545 nm
,Which of the following formulas is an expression of the Beer Lambert law that is routinely
applied to spectrophotometric analysis? correct answers Au X Cs / As = Cu
In spectrophotometry, which of the following is a mathematical expression of the relationship
between absorbance and transmittance? correct answers A = 2 - log % T
Which of the following is not a problem inherent in turbidimetry? correct answers Need to
maintain a constant and specific temperature
Which of the following may be associated with reflectance spectrophotometry as it relates to the
dry reagent slide technique? correct answers Unabsorbed, reflected light detected by
photodetector
Fluorometers are designed so that the path of the exciting light is at a right angle to the path of
the emitted light. What is the purpose of this design? correct answers Prevent excitation light
from reaching the detector
Which of the following represents a primary advantage of performing fluorometric over
absorption spectroscopic methods of analysis? correct answers Increased specificity and
increased sensitivity
Which of the following may be associated with fluorescence polarization? correct answers Plane
polarized light is used for sample excitation
Which of the following may be associated with bioluminescence? correct answers Light
emission produced due to enzymatic oxidation of a substrate
Nephelometry is based on the measurement of light that is: correct answers Scattered by particles
in suspension
,Which of the following instruments is used in the clinical lab or in reference labs to detect beta
and gamma emissions? correct answers Scintillation counter
Which of the following best describes chemiluminescence? correct answers Chemical energy
excites electrons that emit light upon return to ground state
In assaying an analyte with a single beam atomic absorption spectrophotometer, what is the
instrument actually measuring? correct answers Intensity of the beam from the hollow cathode
lamp after it has passed through the analyte containing flame
What is the function of the flame in atomic absorption spectroscopy? correct answers Bring the
metal analyte to its ground state
Most atomic absorption spectrophotometers incorporate a beam chopper and a tuned amplifier.
The purpose of these components is to avoid errors that would be caused by: correct answers
Measurement of light emitted by the analyte
In potentiometry, which of the following is considered the standard electrode? correct answers
Hydrogen electrode
In an electrolytic cell, which of the following is the half cell where reduction takes place? correct
answers Cathode
Mercury covered by a layer of mercurous chloride in contact with saturated potassium chloride
solution is a description of which of the following types of electrodes? correct answers Calomel
When a pH sensitive glass electrode is not actively in use, in what type of solution should it be
kept? correct answers The medium recommended by the manufacturer
, When measuring K positive with an ion selective electrode by means of a liquid ion exchange
membrane, what antibiotic will be incorporated into the membrane? correct answers
Valinomycin
Which of the following is false about ion selective electrode analysis of sodium? correct answers
Membrane coated with valinomycin
What are the principles of operation for a chloride analyzer that generates silver ions as part of
its reaction mechanism? correct answers Amperometry and coulometry
When quantifying glucose using an amperometric glucose electrode system, which of the
following is not a component of the system? correct answers Hexokinase reacts with glucose
To calibrate the pH electrode in a pH blood gas analyzer, it is necessary that: correct answers
Two buffer solutions of known pH be used
The measurement of CO2 in blood by means of a PCO2 electrode is dependent on the: correct
answers Change in pH because of increased carbonic acid in the electrolyte surrounding the
electrodes
The measurement of oxygen in blood by means of a PO2 electrode involves which of the
following? correct answers Direct relationship between amount of oxygen in the sample and
amount of current flowing in the measuring system
Which of the following blood gas parameters are measured directly by the blood gas analyzer
electrochemically as opposed to being calculated by the instrument? correct answers pH, PCO2,
PO2
Which of the following statements is false about anodic stripping voltammetry (ASV)? correct
answers Based on potentiometry
