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Applied Science Unit 2 exam with precise detailed answers

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Applied Science Unit 2 exam with precise detailed answers

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Applied Science Unit 2 exam with
precise detailed answers

Suggest why laboratory technicians must complete health and safety training before they are
|||||| |||||| |||||| |||||| |||||| |||||| |||||| |||||| |||||| |||||| |||||| |||||| ||||||




allowed to work in the laboratory - Correct answer ✔to ensure that they are aware of the risk and
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hazards in laboratories and how to deal with them.
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Information that must be recorded for samples taken - Correct answer ✔date
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name of person handling samples
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location (where sample is from and where it is transported to)
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Flame test colours - Correct answer ✔Barium (Ba2+)= Pale Green
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Copper= Green Blue |||||| |||||| ||||||




Lithium= Crimson Red |||||| ||||||




Sodium (Na+)= Yellow |||||| ||||||




Potassium (K+)= Lilac |||||| ||||||




Calcium (Ca2+)= Orange-red |||||| ||||||




Properties of a suitable internal standard - Correct answer ✔Internal standard is chemically related
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to the compounds that are being analysed but it should not be naturally present in the sample.
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Compounds which have the same functional groups. |||||| |||||| |||||| |||||| |||||| |||||| ||||||




Why are tubes containing blood samples enclosed in plastic watertight containers - Correct answer
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✔Prevents degradation by water. |||||| |||||| ||||||

, Two containers limits possibility of loss sample if one container is broken.
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2 ways of reducing risks associated with hazards in labs - Correct answer ✔Carry out risk
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assessment ||||||




Use PPE for protection
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Train staff in necessary procedures
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Comparing light and electron microscopes - Correct answer ✔Light Microscope |||||| |||||| |||||| |||||| |||||| |||||| |||||| |||||| ||||||




•Rely on light rays |||||| |||||| ||||||




•Typically magnify objects by 1000 to 2000 X |||||| |||||| |||||| |||||| |||||| |||||| ||||||




•Images seen in 2D |||||| |||||| ||||||




•Live specimen can be seen
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Electron Microscope |||||| ||||||




•Electrons bombarded using high levels of energy |||||| |||||| |||||| |||||| |||||| ||||||




•Specimen is killed |||||| |||||| ||||||




•Colours cannot be detected |||||| |||||| |||||| ||||||




•Magnification roughly 2,000,000 X |||||| |||||| ||||||




How can the width of human hair be calculated using a graticule and a Light Microscope - Correct
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answer ✔•Mount hair on Microscope slide,use sellotape to hold the hair down.
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•Measure number of divisions corresponding to width of hair on graticule.|||||| |||||| |||||| |||||| |||||| |||||| |||||| |||||| |||||| ||||||




•Look through graticule at a calibration slide to find number of divisions on calibration slide
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corresponding to divisions on graticule. |||||| |||||| |||||| |||||| ||||||




•Calculate the true distance corresponding to divisions. |||||| |||||| |||||| |||||| |||||| ||||||




•Calculate width of hair. |||||| |||||| ||||||

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