Bio 205 Final Exam Practice Questions and Answers 2025

Bio 205 Final Exam Practice Questions and
Answers 2025
L21/22AIfAyouAperformAtwoAtrialsAforAfiveAsubjectsAandAcalculateAHeartARateAbeforeA(basal)A
andAafterAapplicationAofAtheAvariableA(peak),AyouAcanAanalyzeAyourAdataAinAallAtheAfollowin
gAwaysAExcept?A

A.ASubjectA5'sAmeanApeakAheartArateAandAitsAstandardAdeviationA

B.AMinimumAbasalAandApeakAheartAratesAforAallAfiveAsubjectsA

C.At-TestAcomparingAbasalAandApeakAheartAratesA

D.A%AdifferenceAbetweenAbasalAandApeakAheartAratesA

E.ATheAvariabilityAinAbasalAheartArateAversusApeakAheartArateA-ACORRECTAANSWERSA-
A.ASubjectA5'sAmeanApeakAheartArateAandAitsAstandardAdeviation
3.AL24AHowAdoesAPND496AdifferAfromAPND434?A

A.APND496AcontainsAtheAfull-lengthARFPAgene,APND434AdoesAnot.A

B.ATransformationAwithAPND434AallowsAyeastAcells,AauxotrophicAforAleucine,AtoAgrowAonAS
C-leuAplates,AbutAtransformationAwithApND496AdoesAnot.A

C.APND496AhasAtheAnucleotideAsequenceAforAtheAguideARNAAthatAtargetsAtheARFPAgene,A
butAPND434AdoesAnot.A

D.APND496AcontainsAtheAgeneAforACas9,AbutApND434AdoesAnot.A

E.APND496AcausesAyeastAcellsAtoAfluoresceAred,APND434AdoesAnot.A-
ACORRECTAANSWERSA-

C.APND496AhasAtheAnucleotideAsequenceAforAtheAguideARNAAthatAtargetsAtheARFPAgene,A
butAPND434AdoesAnot.
4.AL25AWhenAyeastAstrainARJY29A(RFPAgeneAandAauxotrophicAforAleucineAatAtheALeu2Aloc
us)AisAtransformedAbyAPDN496,AwhichAofAtheAfollowingAdoesANOTAoccur?A

A.ATheAPND496ADNAAsuccessfullyAtraversesAtheAyeastAcellAwall,AplasmaAmembrane,AandA
cytoplasmAtoAtheAnucleus.A

B.ATheAGRFP2ADNAAisAtranscribedAinAtheAnucleusAandAitsARNAAtranslatedAinAtheAcytoplas
m.A

C.ATheACas9AproteinAisAtransportedAfromAtheAcytoplasmAtoAtheAnucleus,A

, D.ATheALEU2AgeneAisAtranscribedAinAtheAnucleusAandAitsARNAAtranslatedAinAtheAcytoplasm
.A

E.ATheACas9AgeneAisAtranscribedAinAtheAnucleusAtoAproduceAtheACas9ARNA.A-
ACORRECTAANSWERSA-

B.ATheAGRFP2ADNAAisAtranscribedAinAtheAnucleusAandAitsARNAAtranslatedAinAtheAcytoplas
m.
5.AL26AYouAsynthesizeAaAguideARNAAthatAbaseApairsAwithAtheAcomplementaryAstrandAofAth
eAtargetAsequenceA(includesAPAMAsite):A5'ATGAAAGCTAGACAGCGTGCCTGGA3'AWhich
AofAtheAfollowingAsequencesAshowsAtheAcorrectAGRNA,AafterAitAisAtranscribed,AwrittenAinAth

eA5'AtoA3'Adirection?A3'.A

A.A5'ACUUUCGAUGUGUCGCACGGA3A

B.A5'GGCACGCUGUGUAGCUUUCAA3'A

C.A5'TGAAAGCTAGACAGCGTGCCA3A

D.A5'ACTTTCGATCTGTCGCACGGACCA3A

E.A5'UGAAAGCUAGACAGCGUGCCA3A-ACORRECTAANSWERSA-
E.A5'UGAAAGCUAGACAGCGUGCCA3
6.AL25AYouAuseAtheACRISPR/Cas9AsystemAtoAmakeAaAspecificAdeletionA(GeneAXA)AusingA
PCR.ATheAprimerAlocationsAforAtheAfull-
lengthAdeletionAinAGeneAX.AYouAtestAforAtheApresenceAofAtheAGeneAXAandAforAGeneAXAAare
:AGeneAXAForwardAprimerA(1020-1045)AandAReverseAprimerA(1800-
1827);AGeneAXAAForwardAprimerA(1020-1045)AandAReverseAprimerA(1540-
1565).AHowAbigAisAtheAampliconAforAtheAdeletionA(GeneAXA)?A

A.A526AВ.A525AC.A520AD.A546AE.A545A-ACORRECTAANSWERSA-D.A546
7.AL20AEinthoven'sAtriangleAhelpsAusAunderstandAsignalAdeflectionsAinAanAECG,AbothAtheirA
directionAandAamplitude.ATheA"QAwave"AdeflectionAisAdueAmostAlikelyAtoAtheAfactAthatA

A.AAAdepolarizingAwaveAthroughAtheAheartAisAtravelingAtowardsAtheA"_"AECGAelectrodeA

B.AAAdepolarizingAwaveAthroughAtheAheartAisAtravelingAtowardsAtheA"+"AECGAelectrodeA

C.AAArepolarizingAwaveAthroughAtheAheartAisAtravelingAtowardsAtheA"+"AECGAelectrodeA

D.AAArepolarizingAwaveAthroughAtheAheartAisAtravelingAtowardsAtheA"-"AECGAelectrodeA

E.AAAdepolarizingAwaveAthroughAtheAheartAisAtravelingAperpendicularAtoAtheA"+"AelectrodeA-
ACORRECTAANSWERSA-

A.AAAdepolarizingAwaveAthroughAtheAheartAisAtravelingAtowardsAtheA"_"AECGAelectrode