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Microbiology Lab Practical – Final Exam Questions And Answers 100% Pass| Graded A+

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1.Which medium was most difficult to prepare without contamination? Why do think this might be so? - answer Quandrant streak method. It was important to keep the lid at a 45 degree while you streak but I found that to be difficult and I could have easily contaminated by anything in the air. 2.What is the point of contamination in: all broth tubes - answer the autoclave broke broth tube - answer accidents happen h on only the surface of a plate - answer after it hardened, only on surface ghout the agar's thickness on a plate - answer while poured or while still liquid h only in the upper 1 cm of agar in an agar deep tube - answer before it hardened plates in a batch have the same type and density of contaminants - answer stock bottle was contaminated a few flates in a batch are contaminated and each looks different - answer after poured and by air 9.Did you achieve isolation using the quadrant streak? if so, in which streak (1,2,3, or 4) did it occur? If you did not achieve isolation, what might you do differently next time to improve your results? - answer quadrant, mostly 4, sometimes 3 10.On which plate did you obtain isolation with ? How about S. marcescens? Do you have reason to suspect that they should become isolated on the same dilution plate? Why or why not? - answer Serratia Marcescens grew with higher dilution 11.Once you obtained isolation at a particular dilution, did you continue to have isolation on subsequent dilution plates? Is this what you would expect? Why or why not? - answer Continue to be isolated 12.What is the consequence of not spreading the inoculum adequately over the agar surface? - answer They won't isolate as well, clumped

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MICROBIOLOGY FUNDAMENTALS A CLINICAL
APPROACH, 4TH EDITION CHAPTER 1 COMPLETE
NEWEST VERIFIED VERSION

1.Microbiology - answer A specialized area of biology that deals with living things
ordinarily too small to be seen without magnification.


2.Microorganisms include: - answer Bacteria, archaea, fungi, and protozoa


3.Helminths - answer Multicellular animals whose mature form is visible to the
naked eye.


4.Virus and prions - answer Acellular organisms that cause human disease


5.Bacterium/ archaeon and eukaryote - answer Cellular organisms


6.Eukaryotic cell - answer Contains a nucleus


7.Prokaryotic cell or akaryote - answer Lacks the nucleus


8.Three domains - answer Bacteria, Archaea, Eukarya


9.ubiquitous - answer found everywhere

,10.Theory of evolution - answer The accumulation of changes that occur in
organisms as they adapt to their environments.


11.Alexander fleming - answer Discovered penicillin. Also discovered that mold
generated a substance that kills bacteria


12.Biotechnology - answer The manipulation of living organisms or their
components to produce useful products.


13.Genetic engineering - answer Manipulating the genetics of microbes, plants
and animals to create new products. Called GMO's


14.Bioremediation - answer The use of organisms to detoxify and restore
polluted and degraded ecosystems.


15.Pathogens - answer disease causing agents


16.Infectious disease - answer disease caused by a microorganism


17.Which disease processes has microbial infection been implicated? - answer
Gastric ulcers, diabetes type 1, schizophrenia, and obesity.


18.Organelles - answer Small membrane-bound structures in the eukaryotic cell
that perform specific functions and include the nucleus, mitochondria, and
chloroplasts.

, 19.spontaneous generation - answer the belief that invisible vital forces present
in matter led to the creation of life


20.What three consumables are made up of bacteria and fungi - answer Wine
cheese and bread


21.Abiogenesis - answer A="without"; bio="life"; genesis="beginning"
Beginning in absence of life. Embraces the idea of spontaneous generation.


23.Biogenesis - answer Beginning with life
Embracing that living things arise only from others of their same kind.


24.Louis Pasteur - answer Discovered fermentation
Invented "swan neck" flask which DISPROVED spontaneous generation.
Developed first vaccine based on attenuated strains of microbes.


25.Robert Hooke - answer coined the term "cell"


26.Antonie Van Leeuwenhoek - answer Invented the microscope, establishing
that there is life smaller than the eye can see. Father of microbiology. Scraped
from his teeth to gather bacteria.


27.Which of the following is NOT a domain of the Woese-fox system of taxonomy?
- answer Protista

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