BLD 313 Actual Exam Questions With
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!
1. A cuvette of absorption spectrophotometry can be made of polystyrene
(plastic), glass, quartz, etc. How do we choose the type of Corvette? -
ANSWER It is based on the wavelength we need to detect.
2. Which of the following can be done to increase the specificity as well as the
sensitivity of spectrophotometric analysis? - ANSWER Narrow the band
pass
3. Which of the following represents a primary advantage of performing
fluorometric over absorption spectroscopic methods of analysis? - ANSWER
Increased specificity and increased sensitivity
4. Flurometers are designed so that the path of the exciting light is at a right
angle to the path of the emitted light. What is the purpose of this design? -
ANSWER Prevent excitation light from reaching the detector
5. Which of the following is measured in time-delayed fluorescence? -
ANSWER Phosphorescence
6. Which of the following statements is true about fluorescent polarization? -
ANSWER It can be used in competitive-binding assay.
, 7. Which of the following techniques detects unabsorbed, reflected light by a
photodetector? - ANSWER Reflectance photometry
8. Which of the following is used for urine dip stick reading? - ANSWER
Reflectance photometry
9. Which of the following is measured in turbidimetry? - ANSWER
Reduction of transmitted light due to scatter
10.Which of the following is measured in nephelometry? - ANSWER
Scattered light
11.Which of the following statement is true about nephelometry? - ANSWER
The concentration to be measured is directly proportional to scattered
light.
12.Which of the following is associated with chemiluminescence? - ANSWER
The chemical reaction produces an intermediate compound (excited
electron), which becomes stable final product (ground state electron). A
light is emitted when the excited electron goes back to the ground state.
13.Which of the following is associated with a bioluminescence? - ANSWER
The enzyme reaction produces an intermediate compound (excited
electron), which becomes stable final product (ground state electron). A
light is emitted when the excited electron goes back to the ground state.
Reviewed 100% Correct Detailed Answers
Guaranteed Pass!!
1. A cuvette of absorption spectrophotometry can be made of polystyrene
(plastic), glass, quartz, etc. How do we choose the type of Corvette? -
ANSWER It is based on the wavelength we need to detect.
2. Which of the following can be done to increase the specificity as well as the
sensitivity of spectrophotometric analysis? - ANSWER Narrow the band
pass
3. Which of the following represents a primary advantage of performing
fluorometric over absorption spectroscopic methods of analysis? - ANSWER
Increased specificity and increased sensitivity
4. Flurometers are designed so that the path of the exciting light is at a right
angle to the path of the emitted light. What is the purpose of this design? -
ANSWER Prevent excitation light from reaching the detector
5. Which of the following is measured in time-delayed fluorescence? -
ANSWER Phosphorescence
6. Which of the following statements is true about fluorescent polarization? -
ANSWER It can be used in competitive-binding assay.
, 7. Which of the following techniques detects unabsorbed, reflected light by a
photodetector? - ANSWER Reflectance photometry
8. Which of the following is used for urine dip stick reading? - ANSWER
Reflectance photometry
9. Which of the following is measured in turbidimetry? - ANSWER
Reduction of transmitted light due to scatter
10.Which of the following is measured in nephelometry? - ANSWER
Scattered light
11.Which of the following statement is true about nephelometry? - ANSWER
The concentration to be measured is directly proportional to scattered
light.
12.Which of the following is associated with chemiluminescence? - ANSWER
The chemical reaction produces an intermediate compound (excited
electron), which becomes stable final product (ground state electron). A
light is emitted when the excited electron goes back to the ground state.
13.Which of the following is associated with a bioluminescence? - ANSWER
The enzyme reaction produces an intermediate compound (excited
electron), which becomes stable final product (ground state electron). A
light is emitted when the excited electron goes back to the ground state.
