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ASU MIC 205 PENTON EXAM 1 NEWEST ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
ANSWERS (VERIFIED ANSWERS) |ALREADY
GRADED A+|| NEW VERSION!!!
What is the difference between a simple and differential
stain? - Answer-A simple stain uses only one dye to color
all cells in a sample, allowing observation of basic cell
morphology like shape and arrangement, while a
differential stain uses multiple dyes to differentiate
between different types of cells based on their structural
properties, enabling identification of specific bacterial
species or cell components
What is the difference between the kind of information a
simple stain provides and a differential stain provides? -
Answer-A simple stain provides basic information about
cell appearance, while a differential stain allows for more
detailed differentiation between cell types
What is an example of a differential stain? - Answer-A
gram stain
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What is an example of a simple stain? - Answer-Using
methylene blue to identify the morphology of fusiform and
spirochetes in oral infections
What is an important thing to note about simple stains? -
Answer-Simple stains make all of the organisms in a
sample appear to be the same color, even if the sample
contains more than one type of organism
What does a Gram stain detect? - Answer-Peptidoglycan
How does a Gram stain differentiate bacterial cells? -
Answer-It differentiates bacterial cells by their cell wall
composition (chemical and physical properties), either
gram + or gram -
What color is a gram + organism? - Answer-blue/purple
What color is a gram - organism? - Answer-red/pink
What are the principle differences between a gram - and
gram + bacterial cell? - Answer-Gram + = very thick outer
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peptidoglycan layer that makes up 90% of cell weight,
have techoic acids, some also have mycolic acids
Gram - = two membranes, thinner layer of peptidoglycan
that only makes up 10% of cell weight, outer layer of
membrane is made up of lipids
What are teichoic acids? - Answer-Major surface antigens
found on the cell walls of most Gram + bacteria, allowing
the immune system to identify and react against these
bacteria (antigenic determinants and species
identification)
Would a gram - or a gram + bacterial cell be better at
surviving hypertonic and hypotonic solution? - Answer-
Gram - due to its double membrane
What are endotoxins? - Answer-Toxins present inside a
bacterial cell that require the cell to die in order to be
released
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What are exotoxins? - Answer-Toxins that are produced
inside a bacterial cell and exported out, have a dose-
dependent mechanism and contain quorum sensors
What are the purpose of quorum sensors? - Answer-Helps
exotoxins wait to be released until they can cause the
most damage
What do penicillin antibiotics act on within the bacterial
cell? - Answer-Peptidoglycan layer/the ability to produce
peptidoglycan
Would a gram + or gram - cell be more sensitive to
exposure to an antibiotic that acts on the peptidoglycan
layer, such as penicillin? - Answer-Gram+ is more
susceptible since peptidoglycan is exposed to the outer
layer, compared to gram - which has peptidoglycan layer
sandwiched between its two membranes
Why don't penicillin antibiotics harm eukaryotes? -
Answer-Penicillin antibiotics target cell wall structure/ability
to build wall, which is a process that is not present in
eukaryotic cells (eukaryotic cells do not have cell walls
and do not contain peptidoglycan)
ASU MIC 205 PENTON EXAM 1 NEWEST ACTUAL
EXAM COMPLETE 200 QUESTIONS AND CORRECT
ANSWERS (VERIFIED ANSWERS) |ALREADY
GRADED A+|| NEW VERSION!!!
What is the difference between a simple and differential
stain? - Answer-A simple stain uses only one dye to color
all cells in a sample, allowing observation of basic cell
morphology like shape and arrangement, while a
differential stain uses multiple dyes to differentiate
between different types of cells based on their structural
properties, enabling identification of specific bacterial
species or cell components
What is the difference between the kind of information a
simple stain provides and a differential stain provides? -
Answer-A simple stain provides basic information about
cell appearance, while a differential stain allows for more
detailed differentiation between cell types
What is an example of a differential stain? - Answer-A
gram stain
,2|Page
What is an example of a simple stain? - Answer-Using
methylene blue to identify the morphology of fusiform and
spirochetes in oral infections
What is an important thing to note about simple stains? -
Answer-Simple stains make all of the organisms in a
sample appear to be the same color, even if the sample
contains more than one type of organism
What does a Gram stain detect? - Answer-Peptidoglycan
How does a Gram stain differentiate bacterial cells? -
Answer-It differentiates bacterial cells by their cell wall
composition (chemical and physical properties), either
gram + or gram -
What color is a gram + organism? - Answer-blue/purple
What color is a gram - organism? - Answer-red/pink
What are the principle differences between a gram - and
gram + bacterial cell? - Answer-Gram + = very thick outer
,3|Page
peptidoglycan layer that makes up 90% of cell weight,
have techoic acids, some also have mycolic acids
Gram - = two membranes, thinner layer of peptidoglycan
that only makes up 10% of cell weight, outer layer of
membrane is made up of lipids
What are teichoic acids? - Answer-Major surface antigens
found on the cell walls of most Gram + bacteria, allowing
the immune system to identify and react against these
bacteria (antigenic determinants and species
identification)
Would a gram - or a gram + bacterial cell be better at
surviving hypertonic and hypotonic solution? - Answer-
Gram - due to its double membrane
What are endotoxins? - Answer-Toxins present inside a
bacterial cell that require the cell to die in order to be
released
, 4|Page
What are exotoxins? - Answer-Toxins that are produced
inside a bacterial cell and exported out, have a dose-
dependent mechanism and contain quorum sensors
What are the purpose of quorum sensors? - Answer-Helps
exotoxins wait to be released until they can cause the
most damage
What do penicillin antibiotics act on within the bacterial
cell? - Answer-Peptidoglycan layer/the ability to produce
peptidoglycan
Would a gram + or gram - cell be more sensitive to
exposure to an antibiotic that acts on the peptidoglycan
layer, such as penicillin? - Answer-Gram+ is more
susceptible since peptidoglycan is exposed to the outer
layer, compared to gram - which has peptidoglycan layer
sandwiched between its two membranes
Why don't penicillin antibiotics harm eukaryotes? -
Answer-Penicillin antibiotics target cell wall structure/ability
to build wall, which is a process that is not present in
eukaryotic cells (eukaryotic cells do not have cell walls
and do not contain peptidoglycan)
