mcb4271 module 4 lectures 9-11 Exam Questions And Answers 100% Ve
fied.
Study online at https://quizlet.com/_h64n0a
1. protein synthesis 1. Aminoglycosides
inhibitors 2. Macrolides
3. Tetracyclines
4. Chloramphenicol
5. Oxazolidinones
6. Lincosamides
7. Streptogramins
8. Mupirocin
LINk SM COx
AT 30
MinUtes
2. prokaryotic vs Eukaryotes - 80S; 60S & 40S subunits
eukaryotic ribo-
somes Prokaryotes - protein synthesis is facilitated by *70S* ribosomes; 2 subunits - 50S
& 30S
3. bacterial protein 1. initiation: *30S and 50S* subunits need to come together along with an *mRNA
synthesis steps molecule, mRNA template, and the *first amino acid that is attached to tRNA*
2. tRNA molecule carrying the first amino acid, *formylmethionine,* methylene is
*paired with the start codon* on the mRNA at the *P site.* The AUG start codon on
the mRNA is read by the UAC triplet anticodon on the formyl methionyl tRNA.
3. *tRNA molecule carrying the 2nd amino acid approaches the A site* on the
ribosome and recognizes the second code on the mRNA molecule
4. 1st AA joins the 2nd by a *peptide bond*. *1st tRNA molecule is released from
the E site.*
5. In the *translocation step, the ribosome moves along the mRNA until the 2nd
, mcb4271 module 4 lectures 9-11 Exam Questions And Answers 100% Ve
fied.
Study online at https://quizlet.com/_h64n0a
tRNA is in the P site,* and the process continues.
6. *elongation:* ribosome continues to move along the mRNA molecule and the
new AAs are added to the growing polypeptide chain.
7. *termination: ribosome reaches stop codon; polypeptide is released* from the
ribosome
8. recycle: last tRNA is released and the ribosome comes apart.
4. formylmethion- first amino acid in protein synthesis in a bacteria
ine
- Fmethionine is removed post-translationally
- Its presence in humans indicates presence of foreign bodies and triggers the
immune response.
5. aminoglycosides - class of antibiotics that *target protein synthesis*
- structure has *amino sugars linked by glycositic bonds*
- aka *oligosaccharide antibiotics*
- *Streptomycin, neomycin, kanamycin, gentamicin*
- often used in *combination with beta-lactams* -> *synergistic effect* -> bacteri-
cidal
, mcb4271 module 4 lectures 9-11 Exam Questions And Answers 100% Ve
fied.
Study online at https://quizlet.com/_h64n0a
6. aminoglycoside aminoglycosides that were derived from *Streptomyces have mycin suffix*
nomenclature
aminoglycosides derived from *Micromonospora end with micin*
7. mechanism of ac- *amino groups are positively charged* at neutral pH, which *allows them to
tion of aminogly- interact with negatively charged phosphate backbone of target RNA*, and also in
cosides the *30S ribosomal subunit*
- *bind to the 30S ribosomal subunit leads to inhibition of translocation of peptidyl
tRNA* molecule from the A site to the P site and causes *misreading of mRNA,
leaving bacterium unable to synthesize essential proteins*
- *increase mistranslation frequency to 1 to 100 amino acids* -> compromise
proteins stability and lead to *misfolding, aggregation, and loss of function*. For
*essential proteins, that means death*
8. streptomycin Aminoglycoside
- Selman *Waksman* discovered streptomycin in *1944*
- *1st effective medicine for TB*
9. neomycin - discovered by *Waksman*
- one of the *most toxic of all aminoglycoside antibiotics* & *not readily absorbed
through the digestive system* -> restricted to *topical applications only*
fied.
Study online at https://quizlet.com/_h64n0a
1. protein synthesis 1. Aminoglycosides
inhibitors 2. Macrolides
3. Tetracyclines
4. Chloramphenicol
5. Oxazolidinones
6. Lincosamides
7. Streptogramins
8. Mupirocin
LINk SM COx
AT 30
MinUtes
2. prokaryotic vs Eukaryotes - 80S; 60S & 40S subunits
eukaryotic ribo-
somes Prokaryotes - protein synthesis is facilitated by *70S* ribosomes; 2 subunits - 50S
& 30S
3. bacterial protein 1. initiation: *30S and 50S* subunits need to come together along with an *mRNA
synthesis steps molecule, mRNA template, and the *first amino acid that is attached to tRNA*
2. tRNA molecule carrying the first amino acid, *formylmethionine,* methylene is
*paired with the start codon* on the mRNA at the *P site.* The AUG start codon on
the mRNA is read by the UAC triplet anticodon on the formyl methionyl tRNA.
3. *tRNA molecule carrying the 2nd amino acid approaches the A site* on the
ribosome and recognizes the second code on the mRNA molecule
4. 1st AA joins the 2nd by a *peptide bond*. *1st tRNA molecule is released from
the E site.*
5. In the *translocation step, the ribosome moves along the mRNA until the 2nd
, mcb4271 module 4 lectures 9-11 Exam Questions And Answers 100% Ve
fied.
Study online at https://quizlet.com/_h64n0a
tRNA is in the P site,* and the process continues.
6. *elongation:* ribosome continues to move along the mRNA molecule and the
new AAs are added to the growing polypeptide chain.
7. *termination: ribosome reaches stop codon; polypeptide is released* from the
ribosome
8. recycle: last tRNA is released and the ribosome comes apart.
4. formylmethion- first amino acid in protein synthesis in a bacteria
ine
- Fmethionine is removed post-translationally
- Its presence in humans indicates presence of foreign bodies and triggers the
immune response.
5. aminoglycosides - class of antibiotics that *target protein synthesis*
- structure has *amino sugars linked by glycositic bonds*
- aka *oligosaccharide antibiotics*
- *Streptomycin, neomycin, kanamycin, gentamicin*
- often used in *combination with beta-lactams* -> *synergistic effect* -> bacteri-
cidal
, mcb4271 module 4 lectures 9-11 Exam Questions And Answers 100% Ve
fied.
Study online at https://quizlet.com/_h64n0a
6. aminoglycoside aminoglycosides that were derived from *Streptomyces have mycin suffix*
nomenclature
aminoglycosides derived from *Micromonospora end with micin*
7. mechanism of ac- *amino groups are positively charged* at neutral pH, which *allows them to
tion of aminogly- interact with negatively charged phosphate backbone of target RNA*, and also in
cosides the *30S ribosomal subunit*
- *bind to the 30S ribosomal subunit leads to inhibition of translocation of peptidyl
tRNA* molecule from the A site to the P site and causes *misreading of mRNA,
leaving bacterium unable to synthesize essential proteins*
- *increase mistranslation frequency to 1 to 100 amino acids* -> compromise
proteins stability and lead to *misfolding, aggregation, and loss of function*. For
*essential proteins, that means death*
8. streptomycin Aminoglycoside
- Selman *Waksman* discovered streptomycin in *1944*
- *1st effective medicine for TB*
9. neomycin - discovered by *Waksman*
- one of the *most toxic of all aminoglycoside antibiotics* & *not readily absorbed
through the digestive system* -> restricted to *topical applications only*
