Next generation
sequencing test guide with
correct questions and
answers 2025-2026
There are many advantages of using the next generation sequencing and technique as
compared with the Sanger sequencing, for example? -correct answerWe can
sequence many DNA molecules in parallel
What are the possible applications of NGS? -correct answerAll of these options
What is the first step you need to do to prepare the bone sample for DNA sequencing? -
correct answerExtract the DNA
When sequencing ancient DNA, what is the next step in the sample preparation after we
have extracted the DNA from the bone? -correct answerPerform end-repair
Why do we skip the fragmentation part when we are sequencing ancient dinner like the
sample? -correct answerBecause the DNA is already partially degraded
What is the purpose of the end repair procedure? -correct answerTo create blunt
ended strands
What is the next step after and repair? -correct answerAdding adenine to the 3' end of
the Strand
Why do we need an adenine overhang? -correct answerTo ensure that the adaptors
bind specifically
sequencing test guide with
correct questions and
answers 2025-2026
There are many advantages of using the next generation sequencing and technique as
compared with the Sanger sequencing, for example? -correct answerWe can
sequence many DNA molecules in parallel
What are the possible applications of NGS? -correct answerAll of these options
What is the first step you need to do to prepare the bone sample for DNA sequencing? -
correct answerExtract the DNA
When sequencing ancient DNA, what is the next step in the sample preparation after we
have extracted the DNA from the bone? -correct answerPerform end-repair
Why do we skip the fragmentation part when we are sequencing ancient dinner like the
sample? -correct answerBecause the DNA is already partially degraded
What is the purpose of the end repair procedure? -correct answerTo create blunt
ended strands
What is the next step after and repair? -correct answerAdding adenine to the 3' end of
the Strand
Why do we need an adenine overhang? -correct answerTo ensure that the adaptors
bind specifically
