MCB 317 Exam 3 Questions with Correct Answers Already Passed

MCB 317 Exam 3 Questions with Correct Answers Already Passed Gene 1 is normally off but is turned on in the presence of progesterone - Answers The hybrid version of the gene containing the gene 1 coding region should be normally off but turned on in the presence of progesterone Gene 2 is constitutively expressed - Answers The hybrid version of the gene containing the gene 2 coding region is constitutively expressed The consensus elements required for transcription of tRNA and 5sRNA genes by RNAPIII: - Answers are found within the tRNA and 5sRNA coding regions What is the general name for the type of DNA elements that allow eukaryotic genes to be turned on under certain specific conditions, such as in the presence of glucocortoid hormones? - Answers Response element As discussed in lecture, virtually any enhancer can work in combination with virtually any promoter - Answers True Which eukaryotic control region typically mediates regulation of transcription? - Answers Enhancer As discussed in lecture, linker scanner analysis defines the borders of a control region - Answers False Transformation and transfection involve putting DNA molecules constructed in the lab into cells. What is the difference between transformation and transfection? - Answers In transformation the DNA is stably maintained and genes on transformed DNA can be expressed over long periods of time; whereas, in transfection the DNA is not stably maintained and genes on transfected DNA are only transiently expressed Based on our discussion in lecture, which DNA control elements are required for activation of transcription by eukaryotic RNAPII in vitro? - Answers Promoter Based on our discussion in lecture, which DNA control elements are required for activation of transcription by eukaryotic RNAPII in vivo? - Answers Promoter and enhancer The promoter region for genes transcribed by which RNAP are contained entirely within the transcribed region? - Answers RNAPIII As discussed in lecture, once heterochromatin is formed it is copied/duplicated without having to be assembled by spreading de novo each cell division - Answers True As discussed in lecture, position effect variegation is a epigenetic phenomenon - Answers True Linker scanner mutations are substitution mutations - Answers True In terms of genes transcribed by RNAPII, no consensus element is found at all genes - Answers True A given eukaryotic gene transcribed by RNAPII can have multiple enhancers - Answers True As discussed in lecture, TBP is required for transcription by all three eukaryotic RNAP - Answers True Transcription activator proteins usually fall into families of proteins. The characteristic of which domain of activators is used to group them into families? - Answers DNA binding domain TBP binds: - Answers TAF proteins, basal transcription factor proteins, TATA box TBP is part of a multisubunit complex called - Answers TFIID Based on the in vitro data discussed in lecture, what is the most likely order in which proteins assemble at the promoter of a gene transcribed by RNAPII? - Answers TFIID, TFIIB, RNAPII What is the goal of footprinting? - Answers To determine the specifi