MB ASCP CONNECT EXAM WITH CORRECT
ACTUAL QUESTIONS AND CORRECTLY
WELL DEFINED ANSWERS LATEST 2025-
2026 ALREADY GRADED A+
What is the rate of mammalian DNA replication?
a. 500 nucleotides per second
b. 100 nucleotides per second
c. 50 nucleotides per second
d. 10 nucleotides per second - ANSWERS-50 nt/s
Blood drawn into what type of tube is compatible for Genetic testing?
a. K2EDTA tube
b. Sodium citrate tube
c. Sodium Heparin tube
,d. Boric acid, sodium formate tube - ANSWERS-K2EDTA tube
In what part of a qPCR Amplification Plot do you take your measurement?
a. Plateau phase
b. Linear phase
c. Exponential phase
d. Background phase - ANSWERS-Exponential phase
After a patient received a bone marrow transplant, what virus is the patient
more susceptible to?
a. HCV
b. CMV
c. HIV
d. HPV - ANSWERS-CMV
All of the following enzymes are part of the DNA replication machinery, EXCEPT:
a. Ligase
b. DNA polymerase
c. Luciferase
,d. Helicase - ANSWERS-Luciferase
Consider a hypothetical mutation involving gene X. Let's say you amplify a
specific exon, say exon 11, of that gene then you cut it with restriction enzyme
W. In a person without the mutation, cutting the gene with restriction enzyme
W generates two fragments of sizes, 100 bp and 250 bp. Suppose a C>T
mutation in gene X deletes a restriction site, yielding a fragment of 350 bp. You
would expect a heterozygous person for gene X to have these fragments on a
restriction gel:
a. +/+ = 350 bp; 250 bp; 100 bp;
b. m/+ = Only the 350 bp
c. m/+ = 350 bp; 250 bp; 100 bp
d. m/m = 350 bp; 250 bp - ANSWERS-m/+ = 350 bp; 250 bp; 100 bp
Which of the following statements are characteristics of the melt curve
analysis? (hint: more than one answer)
a. At the melting point, the probe separates from the target strand and
fluorescence rapidly decreases.
b. The melting temperature of double stranded DNA depends on its base
composition and length.
c. All PCR products for a specific primer pair should have the same melting
temperature.
, d. When hybridization probes are utilized, the temperature is incrementally
decreased while fluorescence is monitored. - ANSWERS-At the melting point,
the probe separates from the target strand and fluorescence rapidly decreases.
The melting temperature of double stranded DNA depends on its base
composition and length.
All PCR products for a specific primer pair should have the same melting
temperature.
In the field of molecular diagnostics, which one of the following genes is
responsible for the synthesis of DNA, promote cell division, and inhibit cell
death?
a. Proto-oncogenes
b. Tumor suppressor genes
c. Oncogenes
d. Mitochondrial genes - ANSWERS-Proto-oncogenes
Microsatellite instability is best described as:
a. The ability of a gene with repeating elements of 8-10 base pairs to randomly
mutate
ACTUAL QUESTIONS AND CORRECTLY
WELL DEFINED ANSWERS LATEST 2025-
2026 ALREADY GRADED A+
What is the rate of mammalian DNA replication?
a. 500 nucleotides per second
b. 100 nucleotides per second
c. 50 nucleotides per second
d. 10 nucleotides per second - ANSWERS-50 nt/s
Blood drawn into what type of tube is compatible for Genetic testing?
a. K2EDTA tube
b. Sodium citrate tube
c. Sodium Heparin tube
,d. Boric acid, sodium formate tube - ANSWERS-K2EDTA tube
In what part of a qPCR Amplification Plot do you take your measurement?
a. Plateau phase
b. Linear phase
c. Exponential phase
d. Background phase - ANSWERS-Exponential phase
After a patient received a bone marrow transplant, what virus is the patient
more susceptible to?
a. HCV
b. CMV
c. HIV
d. HPV - ANSWERS-CMV
All of the following enzymes are part of the DNA replication machinery, EXCEPT:
a. Ligase
b. DNA polymerase
c. Luciferase
,d. Helicase - ANSWERS-Luciferase
Consider a hypothetical mutation involving gene X. Let's say you amplify a
specific exon, say exon 11, of that gene then you cut it with restriction enzyme
W. In a person without the mutation, cutting the gene with restriction enzyme
W generates two fragments of sizes, 100 bp and 250 bp. Suppose a C>T
mutation in gene X deletes a restriction site, yielding a fragment of 350 bp. You
would expect a heterozygous person for gene X to have these fragments on a
restriction gel:
a. +/+ = 350 bp; 250 bp; 100 bp;
b. m/+ = Only the 350 bp
c. m/+ = 350 bp; 250 bp; 100 bp
d. m/m = 350 bp; 250 bp - ANSWERS-m/+ = 350 bp; 250 bp; 100 bp
Which of the following statements are characteristics of the melt curve
analysis? (hint: more than one answer)
a. At the melting point, the probe separates from the target strand and
fluorescence rapidly decreases.
b. The melting temperature of double stranded DNA depends on its base
composition and length.
c. All PCR products for a specific primer pair should have the same melting
temperature.
, d. When hybridization probes are utilized, the temperature is incrementally
decreased while fluorescence is monitored. - ANSWERS-At the melting point,
the probe separates from the target strand and fluorescence rapidly decreases.
The melting temperature of double stranded DNA depends on its base
composition and length.
All PCR products for a specific primer pair should have the same melting
temperature.
In the field of molecular diagnostics, which one of the following genes is
responsible for the synthesis of DNA, promote cell division, and inhibit cell
death?
a. Proto-oncogenes
b. Tumor suppressor genes
c. Oncogenes
d. Mitochondrial genes - ANSWERS-Proto-oncogenes
Microsatellite instability is best described as:
a. The ability of a gene with repeating elements of 8-10 base pairs to randomly
mutate
