BIOS256 OL, Week 2 Lab
Name:
OL Lab 2: Intestinal Glucose Transport: Study a mouse intestine model to diagnose an
infant
Learning Objectives:
• Understand the epithelial model for how glucose is transported across the
mammalian small intestine
• Perform a glucose assay method
• Use an animal model to study the transport of materials across the intestine
• Describe the effect of sodium-potassium ATPase blocker on glucose transport by
the small intestine
• Explain the effect of manipulations of mucosal concentrations of glucose and sodium
on glucose intestinal transport
• Interpret physiological data and apply to clinical cases
In this simulation, you will learn how glucose absorption occurs in the intestine.
You will help treat an infant who has trouble gaining weight. She is experiencing diarrhea, with
elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your
supervisor, suspects this could be related to the poor glucose absorption. You will use a piece of
mouse intestine as your model to study intestinal glucose transport and see how your findings
can be used to diagnose human diseases.
Study glucose transport by measuring its concentration:
, BIOS256 OL, Week 2 Lab
Name:
After clearing out the impurities from the everted intestinal piece, you will expose both the inner
(mucosal) and outer (serosal) sides to glucose solutions of different concentrations, a sodium-
potassium ATPase blocker, and a sodium-free saline solution.
After these treatments, you will measure the glucose levels on both sides, and analyze your data
to understand the active glucose transport through the intestinal epithelium. Also, a step-by-step
interactive summary diagram will help you visualize what happens to all the molecules and
transporters involved.
Will your findings be able to help make the infant well again?
Part 1: Complete Labster- Intestinal Glucose Transport: Study a mouse intestine model to
diagnose an infant
Part 2: Report and Reflection
Purpose: Describe in your own words and in complete sentences, the purpose of this
experiment. (1 point)
The purpose of this experiment is to observe how glucose is absorbed in the intestines.
Also, how it is transported through the small intestines. It can show the effect of how it
manipulates mucosal glucose and sodium concentrations on intestinal glucose transport.
Observations: List 2 observations you have made in this simulation. (2 points)
1: ORT therapy stimulates sodium, glucose, and water absorption, and ORT replaces
fluid lost through diarrhea.
Name:
OL Lab 2: Intestinal Glucose Transport: Study a mouse intestine model to diagnose an
infant
Learning Objectives:
• Understand the epithelial model for how glucose is transported across the
mammalian small intestine
• Perform a glucose assay method
• Use an animal model to study the transport of materials across the intestine
• Describe the effect of sodium-potassium ATPase blocker on glucose transport by
the small intestine
• Explain the effect of manipulations of mucosal concentrations of glucose and sodium
on glucose intestinal transport
• Interpret physiological data and apply to clinical cases
In this simulation, you will learn how glucose absorption occurs in the intestine.
You will help treat an infant who has trouble gaining weight. She is experiencing diarrhea, with
elevated blood sodium concentration and glucose in her feces and urine. Dr. Shaw, your
supervisor, suspects this could be related to the poor glucose absorption. You will use a piece of
mouse intestine as your model to study intestinal glucose transport and see how your findings
can be used to diagnose human diseases.
Study glucose transport by measuring its concentration:
, BIOS256 OL, Week 2 Lab
Name:
After clearing out the impurities from the everted intestinal piece, you will expose both the inner
(mucosal) and outer (serosal) sides to glucose solutions of different concentrations, a sodium-
potassium ATPase blocker, and a sodium-free saline solution.
After these treatments, you will measure the glucose levels on both sides, and analyze your data
to understand the active glucose transport through the intestinal epithelium. Also, a step-by-step
interactive summary diagram will help you visualize what happens to all the molecules and
transporters involved.
Will your findings be able to help make the infant well again?
Part 1: Complete Labster- Intestinal Glucose Transport: Study a mouse intestine model to
diagnose an infant
Part 2: Report and Reflection
Purpose: Describe in your own words and in complete sentences, the purpose of this
experiment. (1 point)
The purpose of this experiment is to observe how glucose is absorbed in the intestines.
Also, how it is transported through the small intestines. It can show the effect of how it
manipulates mucosal glucose and sodium concentrations on intestinal glucose transport.
Observations: List 2 observations you have made in this simulation. (2 points)
1: ORT therapy stimulates sodium, glucose, and water absorption, and ORT replaces
fluid lost through diarrhea.
