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Lab 8 – Western Blot and Protein Quantification

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This lab focused on detecting GST::CaMPARI protein expression using Western blotting, followed by quantification with ImageJ software. Students compared protein expression levels induced by different derepressor molecules (e.g., IPTG vs. lactose) and analyzed results across the class using bar graphs and fold change calculations.

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Institution
North Carolina State University
Course
BIT 410

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BIT 410/510 Lab Week 8

Western Blot and Protein Quantification

, Lab 8 Order of Operations
❖ Recap
❖ Western Blot
❖ Luminescence detection of peroxidase activity
❖ During western blot run:
❖ Stats Review
❖ begin ImageJ with stain-free blot
❖ Western Blot Quantification
❖ using ImageJ to determine which de-repressor molecule produced the most
GST::CaMPARI protein

, Lab 7 SDS-PAGE recap
Can we confirm GST::CaMPARI is present ONLY with SDS-
PAGE? No!
We see bands at ~78 kDa, so we can ~~GUESS~~ that
GST::CaMPARI could be there
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Institution
North Carolina State University
Course
BIT 410

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Uploaded on
March 30, 2025
Number of pages
22
Written in
2023/2024
Type
Class notes
Professor(s)
Bullock
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