Biotechnology Exam 3
verification of proteins uses what kind of blotting? - Answer -western blotting
whats does sds stand for in sds page - Answer -sodium dodecyl sulfate
sds is also known as a - Answer -detergent
true or false sulfate charges are evenly distributed along denatured protein - Answer -true
the sds protein sample is loaded onto? - Answer -PAGE
how is the protein visualized in sds? - Answer -with coomassie stain
how are proteins from sds page transferred to a nitrocellulose membrane - Answer -by using electrical
current
what does sds page do? - Answer -breaks hydrogen bonding and allows protein to take a linear
configuration
sds gel is how many times more fragile than argose gel - Answer -10x more fragile
why is the membrane blocked in sds page? - Answer -so antibody will not bind nonspecificaly
,what grocery store item is used to ensure that protein does not bind filter paper, but to protein of
interest? - Answer -whole milk
which antibody binds to your protein of interest? - Answer -primary antibody
if rabbit was used as the primary antibody what is a suuitable secondary protein - Answer -mouse
antirabbit or
goat anti rabbit
Why is the substrate added? - Answer -to help make reporter visible
what does the substrate reveal? - Answer -visible band where primary antibody is bound to the protein
what perecentage of of bacterial species have been identified, cultured, studied - Answer -less than 1
percent
what percent of earths biomass are bacteria resposible for? - Answer -50 percent
what are examples of microbes - Answer -bacteria
fungi
algae
protozoa
what is also known as the discovery and development of new products from biological sciences - Answer
-bioprospecting
what are examples of microbial enzymes - Answer -taq DNA
cellulase
substilisin
, where is subtilisin found? - Answer -laundry detergents
what is cellulase used for - Answer -stoned washed jeans
make animal food more easily digestible
what does yeast 2 hybrid system determine - Answer -if protein A and B interact with eachother
what is the first plasmid called? - Answer -bait plasmid
what does the bait plasmid consist of? - Answer -DNA binding domain of a transcription factor that binds
to the promoter
the second plasmid is also known as - Answer -fish protein
what does the fish protein consist of? - Answer -an activator domain
what happens if protein a and b interact - Answer -the DNA binding domain and the activator domain
will be brought together
there are how many types of fish plasmid - Answer -thousand
your protein of interest is the fish or bait protein? - Answer -the bait protein
DNA binding proteins binds to what? - Answer -promoter
the activator domain binds to what? - Answer -enhancer
what is the name of the gene that is important for the synthesis of the amino acid histone? - Answer -
HIS responder gene
verification of proteins uses what kind of blotting? - Answer -western blotting
whats does sds stand for in sds page - Answer -sodium dodecyl sulfate
sds is also known as a - Answer -detergent
true or false sulfate charges are evenly distributed along denatured protein - Answer -true
the sds protein sample is loaded onto? - Answer -PAGE
how is the protein visualized in sds? - Answer -with coomassie stain
how are proteins from sds page transferred to a nitrocellulose membrane - Answer -by using electrical
current
what does sds page do? - Answer -breaks hydrogen bonding and allows protein to take a linear
configuration
sds gel is how many times more fragile than argose gel - Answer -10x more fragile
why is the membrane blocked in sds page? - Answer -so antibody will not bind nonspecificaly
,what grocery store item is used to ensure that protein does not bind filter paper, but to protein of
interest? - Answer -whole milk
which antibody binds to your protein of interest? - Answer -primary antibody
if rabbit was used as the primary antibody what is a suuitable secondary protein - Answer -mouse
antirabbit or
goat anti rabbit
Why is the substrate added? - Answer -to help make reporter visible
what does the substrate reveal? - Answer -visible band where primary antibody is bound to the protein
what perecentage of of bacterial species have been identified, cultured, studied - Answer -less than 1
percent
what percent of earths biomass are bacteria resposible for? - Answer -50 percent
what are examples of microbes - Answer -bacteria
fungi
algae
protozoa
what is also known as the discovery and development of new products from biological sciences - Answer
-bioprospecting
what are examples of microbial enzymes - Answer -taq DNA
cellulase
substilisin
, where is subtilisin found? - Answer -laundry detergents
what is cellulase used for - Answer -stoned washed jeans
make animal food more easily digestible
what does yeast 2 hybrid system determine - Answer -if protein A and B interact with eachother
what is the first plasmid called? - Answer -bait plasmid
what does the bait plasmid consist of? - Answer -DNA binding domain of a transcription factor that binds
to the promoter
the second plasmid is also known as - Answer -fish protein
what does the fish protein consist of? - Answer -an activator domain
what happens if protein a and b interact - Answer -the DNA binding domain and the activator domain
will be brought together
there are how many types of fish plasmid - Answer -thousand
your protein of interest is the fish or bait protein? - Answer -the bait protein
DNA binding proteins binds to what? - Answer -promoter
the activator domain binds to what? - Answer -enhancer
what is the name of the gene that is important for the synthesis of the amino acid histone? - Answer -
HIS responder gene
