BCH 401G Final Exam Questions And Answers

BCH 401G Final Exam Questions And
Answers
____rbindsrtortheroriCrandrrecruitsr____r(helicase)r-rCORRECTrrANSWER-DnaA;rDnaB

____risrarmorerpermanentrmodificationrofrDNArwhiler_____risrmorertemporaryr-
rCORRECTrrANSWER-CRISPR;rRNAir(RNArinterference)




____risrarstartrcodonrthatrencodesrMetr-rCORRECTrrANSWER-AUG

____rofrtherproteinsrinrE.rcolirDNArreplicationrarerDNArpolymerases,rwhichrcatalyzerthersynt
hesisrofrnewrDNAr-rCORRECTrrANSWER-five

_____rexonucleaseractivityrofrDNArpolymeraserisrnecessaryrforrproofreadingr-
rCORRECTrrANSWER-3'r->r5'




_____rgenesrcanrberexpressedrinrprokaryotesr-rCORRECTrrANSWER-eukaryotic

_____rpromotersrresultrinrmorerproductr-rCORRECTrrANSWER-strong

______rstrandsrofrDNArmovermorerquicklyronrgelrelectrophoresisr-rCORRECTrrANSWER-
shorter,rsmaller

3rsteprcyclerofrPCRr-rCORRECTrrANSWER-denaturation,rannealing,rextension

5'rnucleotiderofrRNArreactsrwithr____rtorformrthercapr-rCORRECTrrANSWER-GTP

5'rresiduesronr5'rcaprarer_______r-rCORRECTrrANSWER-methylated

ar_____rpercentagerofrcellsrtakeruprtherDNAr-rCORRECTrrANSWER-small

arbreakrinronerstrand;rhasrarfreer3'rhydroxylrandrarfreer5'rphosphorylr-
rCORRECTrrANSWER-nick




arcircularrpiecerofrDNArreplicatedrbyrbacteriar-rCORRECTrrANSWER-vector

arcomplexrofrRNArandrprotein;rthersiterwherergeneticrinformationr(inrtherformrofrmRNA)risrtra
nslatedrintorproteinr-rCORRECTrrANSWER-ribosome

,arcompoundrthatrbindsrtorarreceptorrbutrdoesrnotrtriggerrsignalingreffectsr-
rCORRECTrrANSWER-antagonist




arconservedrmoleculerwhoserlevelrgoesruprwhenrcellsrarerstarvedrofrglucoser(inrE.rcolirandri
nrsomersensesrinrhuman)r-rCORRECTrrANSWER-cyclicrAMPr(cAMP)

arDNArerrorrcorrectionrmechanismr-rCORRECTrrANSWER-
polymeraserexonucleaseractivity

arfamilyrofr~50rrelatedrproteins;rincludesrmajorrdrugrtargetsrlikerestrogenrreceptor,randroidrr
eceptor,rglucocorticoidrreceptorr-rCORRECTrrANSWER-nuclearrhormonerreceptors

argeneticrdisorderrcausedrbyrmutationsrinrnuclearrexcisionrrepairrsystemrinrhumansr-
rCORRECTrrANSWER-xerodermarpigmentosum




argrouprofrenzymesrthatrgenerateraminoacyl-tRNAsr-rCORRECTrrANSWER-
aminoacylrtRNArsynthase

argrouprofrRNAsrthatrcarriesraminoracidsr-rCORRECTrrANSWER-tRNA

arpairingrbetweenrtworbasesrinrRNArthatrdoesrnotrfollowrtherwatsonrcrickrbaserpairrrulesr-
rCORRECTrrANSWER-wobblerbaserpair




arprocessrofrmutatingronerorrmorerbasisrinrDNAr-rCORRECTrrANSWER-
siterdirectedrmutagenesis

arproteinrthatrputsrtherslidingrclamprontorDNAr-rCORRECTrrANSWER-clamprloader

arproteinrthatrsurroundsr(notrbind)rarDNArstrandrtorpromoterarcomplexraroundrtherDNAr-
rCORRECTrrANSWER-slidingrclamp




arsequencercutrbyrarrestrictionrendonucleasertorallowrsiterspecificrinsertionrofrforeignrDNAr-
rCORRECTrrANSWER-uniquercloningrsite




arseriesrofrproceduresrusedrtorrecombinerDNArsegmentsr-rCORRECTrrANSWER-
DNArrecombinantrtechnology

arsiterinrDNArwhererE.rcolirtranscriptionrregulatorrproteinsrbindr-rCORRECTrrANSWER-
operatorr(O1randrO2)

arspecializedrRNArpolymeraser-rCORRECTrrANSWER-primase

arstrongrpromoterrisr_____rconsensusr-rCORRECTrrANSWER-average

arsubrpartrofrthernucleus,rarsiterofrribosomerbiosynthesisr-rCORRECTrrANSWER-nucleolus

,arthreerbasersequencerthatrbindsrtorarcomplementaryrcodonrinrmRNAr-
rCORRECTrrANSWER-anticodonrarm




arweakrpromoterrisr_____rdivergentr-rCORRECTrrANSWER-more

AArbecomesrcovalentlyrattachedrtortRNAratrther3'rendrofrthisrstemr-rCORRECTrrANSWER-
acceptorrstem

addracetylrgroupsrtorhistonesrtorallowrthemrtorcomeroffr-rCORRECTrrANSWER-coactivators

addingracetylrgroupsrtorAAsronrhistonesrdoesrwhat?r-rCORRECTrrANSWER-
decreasesrthernetrpositivercharge.rweakeningrinteractionsrwithrtherDNArandrloosensrtherhig
herrorderrstructure

addingracetylrgroupsrtorhistonesrhaverthreerimportantrresults:r-rCORRECTrrANSWER-
1.rreducedraffinityrofrhistonesrforrDNA
2.rtranscriptionrfactorsrinteractrwithrhistoneracetyl-lysines
3.racetyl-lysinerresiduesralsorbindrandrrecruitrchromatinrremodelingrproteins
4.rmovesrchromatinrawayrfromrtranscriptionrsite

addingrmutagenrtorDNAr-rCORRECTrrANSWER-randomrmutagenesis

afterrokazakirfragmentrsynthesisrisrcomplete,rthererisrargaprbetweenrendrofr____randr____r-
rCORRECTrrANSWER-okazakirfragmentrandrRNArprimer




allrribosomesrcontainr_____rsubunitsrofrunequalrsizer-rCORRECTrrANSWER-two

allowrreplicationrofrtherendsrandrpreventrtherchromosomesrfromrbeingrshortenedrwithreachr
replicationrcycle;rallowsrwholerDNArstrandrtorbercopiedr-rCORRECTrrANSWER-telomeres

allowsrsomertRNArmoleculesrtorrecognizermorerthanronercodonr-rCORRECTrrANSWER-
wobblerbaserpair

amplifiesrdesiredrregionsrofrDNAr(betweenrtworprimers)r-rCORRECTrrANSWER-
polymeraserchainrreactionr(PCR)

anr_____rNTrwillrnotrberheldrasrtightlyrinrtherDNArpolymerase'sractiversiter-
rCORRECTrrANSWER-incorrect




anrantagonistrselectiverforrestrogenrreceptorr-rCORRECTrrANSWER-tamoxifen

anrenzymerthatrunwindsrtherdoublerhelixrbyrbreakingrtherhydrogenrbondsrbetweenrthercom
plementaryrbasesr-rCORRECTrrANSWER-DNArhelicase

, anrestrogenrreceptorrisrartyperofr________rreceptorr-rCORRECTrrANSWER-
nuclearrhormone

anrexamplerofrarsinglerstrandedrnucleotiderexcisionrrepairr-rCORRECTrrANSWER-
nucleotiderexcisionrrepairrofrphotodimerizedrpyrimidinerinrhumans

anrexamplerofrsinglerstrandedrdirectrrepairr-rCORRECTrrANSWER-
photochemicalrdimerizationrofrpyrimidinerdimer

anrexceptionrtorcentralrdogmarwhenrRNArvirusesrencoderanrenzymercalledr______r-
rCORRECTrrANSWER-RNArdependentrRNArpolymerase




anrexcisionrendonucleaser-rCORRECTrrANSWER-excinuclease

antibioticrthatrmimicsr3'rsiderofraa-
tRNA,rformsrcovalentrbondrtorthercarboxylicracidrendrofrpeptiderinrribosome,rreleasingrpre-
maturelyrterminatedrpeptide,rcellrwillrdier-rCORRECTrrANSWER-puromycin

approx.r_____rofrallrbacterialrproteinsrremainrformylatedr-rCORRECTrrANSWER-1/2

asrcellsrdivide,rthertelomeresr_____r-rCORRECTrrANSWER-shorten

assembledratrtherbeginningrofrthermRNArcodingrsequencer-rCORRECTrrANSWER-
translationrcomplex

bacterialrmRNArthatrcanrsenserenvironmentalrsignalsrasrwellrasrencoderproteinsrhaver____
__r-rCORRECTrrANSWER-riboswitches

beforercellsrdivide,rerrorsrneedrtorbercorrectrorr_____r-rCORRECTrrANSWER-
errorsrwillrberinherited

bindrandrkeeprtherDNArstrandsrunwoundrfromreachrotherrafterrtherhelicaserhasrseparatedrt
hemr-rCORRECTrrANSWER-singlerstrandrbindingrproteinr(SSB)

bindrsmallrmoleculesrthatrcauserarstructuralrchangerinrtherriboswitchrthatrterminatesrthersyn
thesisrofrthermRNAr-rCORRECTrrANSWER-riboswitches

bindrtorpreventrre-annealingrofrtherE.rcolirhelixr-rCORRECTrrANSWER-SSBP

bindsrtorsinglerstrandrregionsrtorpreventrthercomplementaryrstrandsrfromrreannealingr-
rCORRECTrrANSWER-singlerstrandrbindingrproteinr(SSB)




biologicalrinformationrflowsrfromrDNArtorRNArtorproteinr-rCORRECTrrANSWER-
centralrdogma