ACTUAL Exam Questions and CORRECT
Answers
Oxidative Phosphorylation - CORRECT ANSWER - The process by which ATP is formed
as a result of the transfer of electrons from NADH and FADH₂ to O₂ by a series of electron
carriers.
Proton-motive force - CORRECT ANSWER - transfer of electrons leads to the pumping of
protons. Takes place across the inner mitochondrial membrane that's used to synthesize ATP
VDAC - CORRECT ANSWER - Voltage-dependent anion channel
2 components of the proton motive force - CORRECT ANSWER - 1. membrane potential
2. proton gradient
Phosphoryl transfer potential - CORRECT ANSWER - ΔG°'
(Prime denotes at pH 7)
In Oxicative phosphorylation the electron transfer potential of NADH/FADH₂ is converted to
phosphoryl transfer potential of ATP.
Convention - CORRECT ANSWER - Oxidant + e⁻ -> reductant
Relationship b/n the standard free energy change & the change in reduction potential -
CORRECT ANSWER - ΔG°' = -nF ΔE'₀
where:
n = number of electrons
F = Faraday constant (23.06 kcal mol⁻¹ V⁻)
ΔE'₀ = change in reduction potential (volts)
, ΔG°' = standard free energy change (kcal mol⁻¹)
Pyruvate = oxidant
lactate = reductant - CORRECT ANSWER -
How much energy is released in the reduction of oxygen to water? - CORRECT
ANSWER -
Direction Electrons move - CORRECT ANSWER - •from -ve to +ve
•lower e⁻ affinity to compounds w/ higher e⁻ affinity
•High energy to low energy
The energy is used to pump protons across the inner mitochondrial membrane.
Electron transport chain is a series of energy transformations converting energy from electron
motive force to proton motive force - CORRECT ANSWER -
Electron carriers in the electron transport chain - CORRECT ANSWER - FADH2
NADH
FMN (flavin mononucleotide)
Ubiquinone (coenzyme Q)
Cytochrome c
FeS clusters
Hemes
Copper ions
Ubiquinone (coenzyme Q) - CORRECT ANSWER - •antioxidant used in anti-wrinkle
cream
•2e⁻ carrier
•ex: CoQ-10 Wrinkle cream....I want that.