Chapter 3 Observing Microorganisms Through a Microscope
3.1 Multiple-Choice Questions
1) Which of the following is NOT equal to 1 mm? A) 0.001 m
B) 1,000,000 nm
C) 0.1 cm
D) 100 μm
E) 1,000.000.000 pm
Answer: D Section: 3.1
Bloom's Taxonomy: Remembering
Learning Outcome: 3.1
2) What structure does light pass through after leaving the condenser in a compound light
microscope?
A) ocular lens
B) objective lens
C) specimen D) illuminator Answer: C
Section: 3.2
Bloom's Taxonomy: Remembering
ASMcue Outcome: 2.1
Learning Outcome: 3.2
3) Which of the following pairs is mismatched? A) nigrosin - negative stain
B) methylene blue - simple stain
C) acidic dye - capsule stain D) basic dye - negative stain E) crystal violet - simple stain
Answer: D Section: 3.3
Bloom's Taxonomy: Analyzing
ASMcue Outcome: 8.1
Learning Outcome: 3.7
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4) Which of the following places the steps of the Gram stain in the correct order?
1- Alcohol-acetone
2- Crystal violet
3- Safranin
4- Iodine
A) 1-2-3-4
B) 2-1-4-3
C) 2-4-1-3
D) 4-3-2-1
E) 1-3-2-4
Answer: C Section: 3.3
Bloom's Taxonomy: Applying
ASMcue Outcome: 8.1
Learning Outcome: 3.9
5) Which of the following pairs is mismatched? A) alcohol-acetone - decolorizer
B) crystal violet – basic dye
C) safranin – acid dye
D) iodine – mordant
E) carbolfuchsin – basic dye
Answer: C Section: 3.3
Bloom's Taxonomy: Analyzing
ASMcue Outcome: 8.1
Learning Outcome: 3.7
6) Which of the following is NOT true regarding the acid-fast stain? A) It is used to identify
members of the genus Mycobacterium.
B) Acid-fast cells retain the primary dye after treatment with acid-alcohol. C) If cells are
acid-fast, they are gram-negative.
D) Acid-fast cells appear red in a completed acid-fast stain.
E) Non-acid-fast microbes appear blue in a completed acid-fast stain. Answer: C
Section: 3.3
Bloom's Taxonomy: Remembering
ASMcue Outcome: 8.1
Learning Outcome: 3.10
7) The purpose of a mordant in the Gram stain is to
A) remove the simple stain.
B) make the bacterial cells larger. C) make the flagella visible.
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3.1 Multiple-Choice Questions
1) Which of the following is NOT equal to 1 mm? A) 0.001 m
B) 1,000,000 nm
C) 0.1 cm
D) 100 μm
E) 1,000.000.000 pm
Answer: D Section: 3.1
Bloom's Taxonomy: Remembering
Learning Outcome: 3.1
2) What structure does light pass through after leaving the condenser in a compound light
microscope?
A) ocular lens
B) objective lens
C) specimen D) illuminator Answer: C
Section: 3.2
Bloom's Taxonomy: Remembering
ASMcue Outcome: 2.1
Learning Outcome: 3.2
3) Which of the following pairs is mismatched? A) nigrosin - negative stain
B) methylene blue - simple stain
C) acidic dye - capsule stain D) basic dye - negative stain E) crystal violet - simple stain
Answer: D Section: 3.3
Bloom's Taxonomy: Analyzing
ASMcue Outcome: 8.1
Learning Outcome: 3.7
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4) Which of the following places the steps of the Gram stain in the correct order?
1- Alcohol-acetone
2- Crystal violet
3- Safranin
4- Iodine
A) 1-2-3-4
B) 2-1-4-3
C) 2-4-1-3
D) 4-3-2-1
E) 1-3-2-4
Answer: C Section: 3.3
Bloom's Taxonomy: Applying
ASMcue Outcome: 8.1
Learning Outcome: 3.9
5) Which of the following pairs is mismatched? A) alcohol-acetone - decolorizer
B) crystal violet – basic dye
C) safranin – acid dye
D) iodine – mordant
E) carbolfuchsin – basic dye
Answer: C Section: 3.3
Bloom's Taxonomy: Analyzing
ASMcue Outcome: 8.1
Learning Outcome: 3.7
6) Which of the following is NOT true regarding the acid-fast stain? A) It is used to identify
members of the genus Mycobacterium.
B) Acid-fast cells retain the primary dye after treatment with acid-alcohol. C) If cells are
acid-fast, they are gram-negative.
D) Acid-fast cells appear red in a completed acid-fast stain.
E) Non-acid-fast microbes appear blue in a completed acid-fast stain. Answer: C
Section: 3.3
Bloom's Taxonomy: Remembering
ASMcue Outcome: 8.1
Learning Outcome: 3.10
7) The purpose of a mordant in the Gram stain is to
A) remove the simple stain.
B) make the bacterial cells larger. C) make the flagella visible.
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