CHEM 116 LAB STUDY GUIDE EXAM QUESTIONS WITH
ANSWERS 2025/2026
If you spill a chemical on the floor or the bench, you should: - Notify your TA and obtain
proper clean-up instructions
If you splash a chemical in your face, you should move to the eyewash station and rinse
your face for 5-10 minutes. - True
If you wear prescription glasses, you do not need to wear safety goggles in the lab. -
False
It is a good idea to wash your hands before leaving the laboratory for the day. - True
Only the TA needs to know the location of the fire extinguishers in the lab. - False
Which of the following may be disposed of in the regular trash: - Paper towels
Which pair of items are useful in extinguishing small fires? - fire extinguisher and
blanket
You should always inspect glassware carefully before using to make sure it does not
have any cracks or chips. - True
Carey is using a lead cylinder as a counter weight. He would like to know the mass of
the cylinder, but does not have a balance available. Knowing that his cylinder displaced
a volume of 9.91 mL and that the density of lead is known to be 11.34 g/mL, what is the
mass of his cylinder? - 111.98
Jack and Jill are lab partners. Jill delivered 6.75 mL of a 20.96 % sugar stock solution
using a graduated pipette into a 25-mL volumetric flask. Distilled water was added to the
graduation mark on the flask and mixed well. What was the final concentration of the
resulting solution? - 5.66
Report the volume of liquid contained in the graduated cylinder to the correct number of
decimal places. DO NOT include units in your answer. - 3.09
What are the most dominate intermolecular forces present in acetone? - dipole-dipole
forces
Ammonia (NH3 or NH4OH) molecules are capable of hydrogen bonding. Would this be
an example of a polar or a nonpolar solvent. - polar
Which of the following statements best describes the spot size you should aim for when
spotting your TLC plates with the dyes? - as small as possible
, Which of the following statements best describes the line you will create on the bottom
of your TLC plate? - It should be made in pencil about a centimeter from the bottom of
the plate.
Which of the following answers lists the components for the microcolumn in the order
you should add them. - glass wool, sand, silica gel, solvent and dye, sand
Which statement BEST describes the relationship between the spots that are developed
on the TLC plate and the spots that are separated by the microcolumn? - The spots will
appear in opposite order (ex: blue,pink and pink, blue) on the TLC plate and the
microcolumn because we add solvent from the top in one and the bottom in the other.
Which of the following statements best describes the proper disposal of the
microcolumn? - In the glass waste, without paper towels.
Which of the following statements regarding the use of the Buchner funnel is
INCORRECT? - You are limited to only the amounts of 'wash' solvents that are listed in
the manual.
What should you do if you measure 55.3 mL (59.4 g) of milk? - Record the mass and
press on because you care more about exact mass than exact volume here.
Which of the following statements regarding heating the acetone mixture in exercise 2 is
incorrect? - The beaker should be cool enough that you can remove it from the hot plate
with your bare hands.
What color should be observed when a small drop of basic solution is touched to litmus
paper - blue
Which of the following statements regarding the Benedict's Test for Reducing Sugars is
INCORRECT - This test will give a positive result for any sugar.
How many moles of phosphate were isolated from a sample of milk if 1.399-g of calcium
phosphate were isolated after the completion of exercise 3?
Report your answer to 4 decimal places and WITHOUT units. - .009
A denatured protein will be more soluble in water than a protein in it's native, folded
state. - false
A fat molecule will likely be more soluble in heptane (an organic solvent) then in
aqueous solution. - True
Excess of which of the following reagents should be placed in the designated chemical
waste containers? - Acetone
ANSWERS 2025/2026
If you spill a chemical on the floor or the bench, you should: - Notify your TA and obtain
proper clean-up instructions
If you splash a chemical in your face, you should move to the eyewash station and rinse
your face for 5-10 minutes. - True
If you wear prescription glasses, you do not need to wear safety goggles in the lab. -
False
It is a good idea to wash your hands before leaving the laboratory for the day. - True
Only the TA needs to know the location of the fire extinguishers in the lab. - False
Which of the following may be disposed of in the regular trash: - Paper towels
Which pair of items are useful in extinguishing small fires? - fire extinguisher and
blanket
You should always inspect glassware carefully before using to make sure it does not
have any cracks or chips. - True
Carey is using a lead cylinder as a counter weight. He would like to know the mass of
the cylinder, but does not have a balance available. Knowing that his cylinder displaced
a volume of 9.91 mL and that the density of lead is known to be 11.34 g/mL, what is the
mass of his cylinder? - 111.98
Jack and Jill are lab partners. Jill delivered 6.75 mL of a 20.96 % sugar stock solution
using a graduated pipette into a 25-mL volumetric flask. Distilled water was added to the
graduation mark on the flask and mixed well. What was the final concentration of the
resulting solution? - 5.66
Report the volume of liquid contained in the graduated cylinder to the correct number of
decimal places. DO NOT include units in your answer. - 3.09
What are the most dominate intermolecular forces present in acetone? - dipole-dipole
forces
Ammonia (NH3 or NH4OH) molecules are capable of hydrogen bonding. Would this be
an example of a polar or a nonpolar solvent. - polar
Which of the following statements best describes the spot size you should aim for when
spotting your TLC plates with the dyes? - as small as possible
, Which of the following statements best describes the line you will create on the bottom
of your TLC plate? - It should be made in pencil about a centimeter from the bottom of
the plate.
Which of the following answers lists the components for the microcolumn in the order
you should add them. - glass wool, sand, silica gel, solvent and dye, sand
Which statement BEST describes the relationship between the spots that are developed
on the TLC plate and the spots that are separated by the microcolumn? - The spots will
appear in opposite order (ex: blue,pink and pink, blue) on the TLC plate and the
microcolumn because we add solvent from the top in one and the bottom in the other.
Which of the following statements best describes the proper disposal of the
microcolumn? - In the glass waste, without paper towels.
Which of the following statements regarding the use of the Buchner funnel is
INCORRECT? - You are limited to only the amounts of 'wash' solvents that are listed in
the manual.
What should you do if you measure 55.3 mL (59.4 g) of milk? - Record the mass and
press on because you care more about exact mass than exact volume here.
Which of the following statements regarding heating the acetone mixture in exercise 2 is
incorrect? - The beaker should be cool enough that you can remove it from the hot plate
with your bare hands.
What color should be observed when a small drop of basic solution is touched to litmus
paper - blue
Which of the following statements regarding the Benedict's Test for Reducing Sugars is
INCORRECT - This test will give a positive result for any sugar.
How many moles of phosphate were isolated from a sample of milk if 1.399-g of calcium
phosphate were isolated after the completion of exercise 3?
Report your answer to 4 decimal places and WITHOUT units. - .009
A denatured protein will be more soluble in water than a protein in it's native, folded
state. - false
A fat molecule will likely be more soluble in heptane (an organic solvent) then in
aqueous solution. - True
Excess of which of the following reagents should be placed in the designated chemical
waste containers? - Acetone
